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OfflineJustdoingmything
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Registered: 09/08/21
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Contamination advice
    #27460905 - 09/08/21 07:25 PM (3 years, 4 months ago)

Background: 2 weeks of inoculation into 90 second rice.
10 days of colonization.

Here are the pics:

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I am pretty sure that it's mold. It seems like it's pretty contained to that one area. I think the rest is colonized appropriately. I carefully removed that one area of mold from the container.

I'd love to hear recommendations. Thank you!

Edited by Justdoingmything (09/08/21 07:27 PM)

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Offlinekanemush
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Re: Contamination advice [Re: Justdoingmything]
    #27460915 - 09/08/21 07:36 PM (3 years, 4 months ago)

whats your sterile process from grain to spawn? sub looks really dry so not a moisture issue most likely.

Edited by kanemush (09/08/21 07:37 PM)

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OfflineJustdoingmything
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Re: Contamination advice [Re: kanemush]
    #27460918 - 09/08/21 07:38 PM (3 years, 4 months ago)

I used sterile process but it was weak at best. Also 1/5 of my rice bags grew intense mold.

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OfflineAspiringmycologist
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Re: Contamination advice [Re: Justdoingmything]
    #27460924 - 09/08/21 07:46 PM (3 years, 4 months ago)

There’s most likely something going on with your grains that you’re missing. Check out the pf tek it’s a better option to start from if you’ve got spores

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OfflineCocaineBuffet
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Re: Contamination advice [Re: Aspiringmycologist]
    #27460926 - 09/08/21 07:51 PM (3 years, 4 months ago)

OP said they used microwave rice. Your grains are dirty and you have trich eating your sub. Given the circumstances your tub doesn't look awful but I don't think you will get any fruits before the sub molds over.

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OfflineJustdoingmything
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Re: Contamination advice [Re: CocaineBuffet]
    #27460933 - 09/08/21 07:57 PM (3 years, 4 months ago)

You think it's worth it to introduce fruiting conditions and see how far I get?

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Offlinekanemush
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Re: Contamination advice [Re: Justdoingmything]
    #27460935 - 09/08/21 07:58 PM (3 years, 4 months ago)

if you have the time to experiment yes go ahead and fruit it. I would say bad rice spawn sterile tek is the cause probably nothing else.

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OfflineJustdoingmything
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Re: Contamination advice [Re: kanemush]
    #27460938 - 09/08/21 08:00 PM (3 years, 4 months ago)

Yea, if there's anything that I went wrong on it was the inoculation phase. (2/5 bags grew, 2/5 didn't grow, and 1/5 grew thick mold). This is my first try at this so I was expecting a fair margin of error.

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OfflineCocaineBuffet
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Re: Contamination advice [Re: Justdoingmything]
    #27460941 - 09/08/21 08:06 PM (3 years, 4 months ago)

Go ahead with the experiment but trich is almost always the final nail in the coffin. You got this far might as well see what you can learn from what you got on hand because I assume this is as far as along as your projects are at the current time.

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Offlinekanemush
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Re: Contamination advice [Re: Justdoingmything]
    #27460943 - 09/08/21 08:07 PM (3 years, 4 months ago)

it happens keep trying and follow tek to the tee you will get it figured out just takes time we all fail.

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