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InvisibleCogito Ergo Sum
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Registered: 08/14/21
Posts: 23
Loc: Appalachia
Re: Brown rice and rye spawn jars [Re: coversall]
    #27452613 - 09/01/21 05:55 PM (3 years, 4 months ago)

Just wanted to thank you all again and also update in case you guys were interested. The PF cakes you guys suggested are coming up swimmingly and I couldn't be happier! I inoculated two grain jars just as an experiment to see if I would get any results this time (using rye berries) and unfortunately, no such luck.
However, all five of the BRF and vermiculite jars are showing healthy amounts of rhizomorphic growth!
Thank you guys so much for the recommendations and I'll continue posting my results as they come to me.
You all rock.

Also, please forgive me if I break any forum rules. I think I read them over pretty well but I'm still pretty new to these forums. Hopefully you guys can see the images below ok...






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InvisibleCogito Ergo Sum
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Registered: 08/14/21
Posts: 23
Loc: Appalachia
Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27452650 - 09/01/21 06:38 PM (3 years, 4 months ago)







Reposting after hosting on this site. Just trying to figure out how to post pics on this site. Apologies...

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OfflineMushBrain1
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Registered: 07/05/21
Posts: 132
Last seen: 3 years, 2 months
Re: Brown rice and rye spawn jars [Re: fahtster]
    #27457425 - 09/05/21 05:06 PM (3 years, 4 months ago)

Quote:

fahtster said:
Kneeling in front of a hot af oven to try and do sterile work sounds so uncomfortable :lol:  the only thing I’d even consider possibly suggesting using an oven for is dehydrating mush and even that gives me apprehension.

Reminds me of that pic of that dude in a tyvek suit inoculating pf jars in front of his oven

Faht




Well the proof the Cracked Oven Method rocks is in only 1 bad Agar Dish poured above 250F cracked Oven Door on over-hanging wire Rack.  It defies logic.  Should be shit loads of contam's, yet there are very few!

I have a Still Air Box and use it also.  I wait until my Fridge shuts off for real Still Air, and Wear an N95 Mask.  I do not bother with Cracked Oven Method.  It rocks!  Yes I want a HEPA Flow Box and have zero space for that.

Yes I admit spore syringe to grain or liquid cuture is a poor practice, but I did not know that and succeeded out of ignorance!

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Offlineblackout
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Registered: 07/16/00
Posts: 5,266
Last seen: 1 year, 2 months
Trusted Cultivator
Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27457530 - 09/05/21 06:40 PM (3 years, 4 months ago)

Quote:

Cogito Ergo Sum said: I only have an instant pot and I've never used it to sterilize. I've just cooked them on the stove for about 3 hours with a tight lid on low heat. Initially I thought that was my problem. Now I'm concerned that I overcooked the grains and they dried out too much...




Instantpot is a far better option than steaming in a pot. Most models do get to 15psi intially and if you cancel the cook and restart they should get to 15+psi again and then back off down again. I do not bother doing restarts most times, just set to the max time. There is no babysitting them so it makes no sense for you to use the stove -unless your jars do not fit of course. Some newer IPs do go to higher pressure for the full cook time.

If you insist on using MS syringes direct then use very little. I get 25 drops per ml, so 250 in a 10ml syringe. If there are 10 contams in your syringe and you inject 1cc then there is a good chance one got in, and many contams will outrun the cube myc. If you inject a single drop it typically takes a lot longer to intially grow, but this does not matter too much if you injected no contams with it.

You an weigh your jars and hydrated grains to monitor any moisture loss.

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OnlineThe Tao
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Registered: 09/12/19
Posts: 2,386
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Re: Brown rice and rye spawn jars [Re: blackout] * 1
    #27457727 - 09/05/21 10:21 PM (3 years, 4 months ago)



The first picture is 4 14 gm mason jars. The 2nd is more than that. All from BRF, an instapot and homemade spore syringes.
Listen to what the guys who are making sense say and ignore the others. Everyone has an opinion and that doesn’t help sometimes. The amount of time you’ve spent experimenting has cost you actually producing something.
BRF is by its nature inefficient but it’s also easy. That’s why it’s been around since the 1990’s.
And that’s  why people who just want to have some mushrooms still use it. It works well, not to peak efficiency.
You’ll get some mushrooms, you’ll get contamination, you may get no growth in some jars at all.
Every grow will get better. Then move on if you want to. But you’ll have mushrooms and you’ll have a better idea of what someone is trying to tell you. Basically build a better bullshit meter.
I’ve had to slow down. Started 7 jars every 2 weeks, then moved to every 4 weeks.  I like watching the grows but I have more than enough.
Spore syringes are a complete crap shoot but that doesn’t matter to me. I have more than I need for my purposes and I can grow more, albeit inefficiently, any time I want.

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InvisibleCogito Ergo Sum
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Registered: 08/14/21
Posts: 23
Loc: Appalachia
Re: Brown rice and rye spawn jars [Re: The Tao]
    #27460692 - 09/08/21 04:41 PM (3 years, 4 months ago)

Quote:

The Tao said:

Listen to what the guys who are making sense say and ignore the others. Everyone has an opinion and that doesn’t help sometimes. The amount of time you’ve spent experimenting has cost you actually producing something.




That's why I'm picking everyone's brains here. The cakes are coming along nicely as of today. No sign of contamn and they're about 60% colonized at this point (I screwed up and ground up the rice too fine and it's super compacted within the jars, but still colonizing well).
I think my next step is going to be a BRF to bulk monotub by shredding the cake with a cheese grater into a coir substrate. Which brings me to my next question.
Has anyone messed around with a coir vs CVG substrate and had any noticeably different results? I was thinking of going with just coir, but after reading a few teks it seems it may be beneficial to mix a bit of gypsum and vermiculite into it. I was wondering if you guys had any comments to add to that decision as far as ratios go since I'm a first time grower in an area with very high humidity.
In any case, thank you guys for all the input, I am weighing every option I have as I go.
My next step is to take a few good spore prints to mess around with agar next.
Fingers crossed!

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InvisibleCogito Ergo Sum
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Registered: 08/14/21
Posts: 23
Loc: Appalachia
Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27460699 - 09/08/21 04:46 PM (3 years, 4 months ago)

I'll probably try that next. I first used quart sized jars that wouldn't fit into the instant pot, but since going to BRF cakes in pint sized jars, they'll definitely fit into the pressure cooker. In any case, I haven't had any luck with syringe to grain inoculation. This is the third time I've tried, modifying the preparation methods, and still no sign of growth in those containers. However, I'm extremely happy that my BRF cakes are showing healthy growth. I have a bit of anxiety as far as fruiting them goes, I'm planning on working with a shoebox monotub as of now.
As long as I can harvest a few healthy caps for spores to continue work I'll be happy for the moment...

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OfflineOra
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Registered: 07/09/21
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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27460706 - 09/08/21 04:53 PM (3 years, 4 months ago)

lol anyones pasta consumption increased after they started mush cult?

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OnlineThe Tao
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Registered: 09/12/19
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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27460794 - 09/08/21 06:02 PM (3 years, 4 months ago)

Quote:

Cogito Ergo Sum said:
Quote:

The Tao said:

Listen to what the guys who are making sense say and ignore the others. Everyone has an opinion and that doesn’t help sometimes. The amount of time you’ve spent experimenting has cost you actually producing something.




That's why I'm picking everyone's brains here. The cakes are coming along nicely as of today. No sign of contamn and they're about 60% colonized at this point (I screwed up and ground up the rice too fine and it's super compacted within the jars, but still colonizing well).
I think my next step is going to be a BRF to bulk monotub by shredding the cake with a cheese grater into a coir substrate. Which brings me to my next question.
Has anyone messed around with a coir vs CVG substrate and had any noticeably different results? I was thinking of going with just coir, but after reading a few teks it seems it may be beneficial to mix a bit of gypsum and vermiculite into it. I was wondering if you guys had any comments to add to that decision as far as ratios go since I'm a first time grower in an area with very high humidity.
In any case, thank you guys for all the input, I am weighing every option I have as I go.
My next step is to take a few good spore prints to mess around with agar next.
Fingers crossed!



https://www.shroomery.org/forums/showflat.php/Number/24461957
Proper spore printing. Good read. Answers all the questions you didn’t know you had.

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