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Offlinemushes
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Registered: 02/24/20
Posts: 23
Last seen: 2 years, 8 months
entomopathogenic fungus
    #27446698 - 08/28/21 05:45 PM (2 years, 8 months ago)

Here is an unpdate on the insect I found, colonised with an entomopathogenic fungus:

I isolated the fungus, which appears to be Beauveria / Cordyceps sp. on a variety of media (SDA, MEA, MEPA). What look like Cordyceps stromata grew within two weeks on SDA:


I'm pretty surprised, I wasn't aware that Beauveria sp. will form teleomorphs under these conditions. Does anyone know if this is unusual?


Next step is to check it out under the microscope.

Edited by mushes (09/10/21 08:20 AM)

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Invisiblepablokabute
Hari ng Amag
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Registered: 11/22/11
Posts: 5,187
Loc: rural ghetto
Re: entomopathogenic fungus [Re: mushes]
    #27446850 - 08/28/21 07:49 PM (2 years, 8 months ago)

Im veryyy fascinated with cordyceps spp. so thanks for sharing.

FASCINATING.


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InvisibleBaba Yaga
Psychedelic Minion

Registered: 09/13/20
Posts: 4,241
Loc: On Break Vacation
Trusted Cultivator
Re: entomopathogenic fungus [Re: pablokabute]
    #27446894 - 08/28/21 08:17 PM (2 years, 8 months ago)

That's awesome!

what's sda? sucrose dextrose agar?

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Offlinemushes
Stranger
Registered: 02/24/20
Posts: 23
Last seen: 2 years, 8 months
Re: entomopathogenic fungus [Re: Baba Yaga]
    #27447026 - 08/28/21 09:16 PM (2 years, 8 months ago)

hey Baba Yaga,

SDA is Sabouraud's dextrose agar (peptone - 10g/L, dextrose - 40g/L, agar - 15g/L). some recipes use 20g/L agar powder. It's mainly used for growing dermatophytes, but I have seen it used for Beauveria in the literature so I tried it out.

For MEA (malt extract agar) I used 30g/L malt extract and 15 g/L agar. MEPA was MEA + 5 g/L peptone.

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InvisibleBaba Yaga
Psychedelic Minion

Registered: 09/13/20
Posts: 4,241
Loc: On Break Vacation
Trusted Cultivator
Re: entomopathogenic fungus [Re: mushes]
    #27447033 - 08/28/21 09:20 PM (2 years, 8 months ago)

Thanks, appreciate the info!

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