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OfflineUkdude
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Ride along contam-fuzz ontop of rhizo mycelium
    #27437060 - 08/21/21 09:12 AM (2 years, 8 months ago)

What’s going on with these plates? 11 days in and this fuzz on top of the rhizo growth,what is it and why are they not growing out uniform,I have 20 plates looking exactly like these,also the last plate looks uniform but not strong what’s up with that ?


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OfflineMyco-pathic
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Re: Ride along contam-fuzz ontop of rhizo mycelium [Re: Ukdude]
    #27437217 - 08/21/21 11:22 AM (2 years, 8 months ago)

Looks like you have a different colony filling in the gaps growing over the top.

I don’t think that’s contaminated.

I would consider transferring the rhizo edge to keep it separate.

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OfflineUkdude
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Re: Ride along contam-fuzz ontop of rhizo mycelium [Re: Myco-pathic]
    #27437226 - 08/21/21 11:27 AM (2 years, 8 months ago)

Quote:

Myco-pathic said:
Looks like you have a different colony filling in the gaps growing over the top.

I don’t think that’s contaminated.

I would consider transferring the rhizo edge to keep it separate.



I do and the next plates turn out the exact same

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OfflineMyco-pathic
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Re: Ride along contam-fuzz ontop of rhizo mycelium [Re: Ukdude]
    #27437232 - 08/21/21 11:33 AM (2 years, 8 months ago)

Try to take a smaller cut. I’m a little stumped why this would repeat indefinitely.

That’s the only thing I can think of If I were in your situation.
Smallest cut possible like a speck or even scrape off just a little knife tip of mycelium.

hopefully someone can help you here.

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OfflineMyco-pathic
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Re: Ride along contam-fuzz ontop of rhizo mycelium [Re: Myco-pathic]
    #27437247 - 08/21/21 11:48 AM (2 years, 8 months ago)

I noticed you labeled this t1. That’s only the first transfer. I don’t think it matters if you did it 20 times. That is not quite the same as 20 isolated transfers from one culture to the next.

MS>t1>t2>t3

I believe that’s when you will find the results you’re looking for. Each one gets you closer to A mono.

————————————————
+Added: OK I see your t2 sorry I’m at work trying to help.
I was under the impression that you transferred from one plate to 20 other plates and got 20 of the same results. That might not be the case..

I still think you just have multiple cultures growing on top of each other. Keep isolating if your sure it’s worth it or fruit it and choose a specimen to take a biopsy and isolate from there. Saves a ton of work and plates.

A biopsy alone will not ensure you’ve isolated exactly what you want but it will definitely narrow it down. You could definitely pull a few monos from that and fruit them all again and decided which one will be your keeper.

Edited by Myco-pathic (08/21/21 12:33 PM)

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InvisibleNimpo
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Re: Ride along contam-fuzz ontop of rhizo mycelium [Re: Myco-pathic]
    #27437296 - 08/21/21 12:45 PM (2 years, 8 months ago)

Post this in normal cultivation.

Ive seen the same behavior before and idk whats going on. Ill see nice rhizo and then a strange fluffyness come on over it.

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OfflineUkdude
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Re: Ride along contam-fuzz ontop of rhizo mycelium [Re: Nimpo]
    #27437519 - 08/21/21 04:32 PM (2 years, 8 months ago)

Quote:

Nimpo said:
Post this in normal cultivation.

Ive seen the same behavior before and idk whats going on. Ill see nice rhizo and then a strange fluffyness come on over it.



I posted there,you ever figure it out or just ditch it all and start again

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OfflineMyco-pathic
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Re: Ride along contam-fuzz ontop of rhizo mycelium [Re: Ukdude]
    #27437561 - 08/21/21 05:08 PM (2 years, 8 months ago)

Both of us basically said its normal.

Why start over?

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OfflineUkdude
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Re: Ride along contam-fuzz ontop of rhizo mycelium [Re: Myco-pathic]
    #27437563 - 08/21/21 05:10 PM (2 years, 8 months ago)

Quote:

Myco-pathic said:
Both of us basically said its normal.

Why start over?




Coz I’ve read it’s a contam somewhere,it’s not cube myc,it’s something riding along,I’m just trying to get other peoples experience and knowledge.

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OfflineEclipse3130
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Re: Ride along contam-fuzz ontop of rhizo mycelium [Re: Ukdude]
    #27437806 - 08/21/21 09:25 PM (2 years, 8 months ago)

It's normal when your culture isn't fully isolated down. I would take some more transfers until it looks symmetrical.

Once it is, it's a lot easier to read if it's contaminated or not, here's an example of piggyback mold on an isolated culture, using the same agar mix. Second picture is a transfer or 2 later, selecting the clean sections.





--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"

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OfflineUkdude
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Re: Ride along contam-fuzz ontop of rhizo mycelium [Re: Eclipse3130]
    #27446109 - 08/28/21 09:31 AM (2 years, 8 months ago)

How sure are you that’s was mold,did you ever put any of those plates to grain ?

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OfflineEclipse3130
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Re: Ride along contam-fuzz ontop of rhizo mycelium [Re: Ukdude]
    #27452663 - 09/01/21 06:55 PM (2 years, 8 months ago)

Yes they produced trich, you can tell its mold from the plates alone, how I was able to transfer away from it in a few transfers. The inconsistency of the first plate especially with vertical fluffy growth covering the flatter rhizo growth. At least with the 2nd picture you can tell there's nothing but mycelium and no question of contam


--------------------
"In The Material World One seeks retirement and grows Old
In The Magical World One seeks Enlightenment and grows Wiser
In The Miraculous World One seeks nothing and grows Lighter
As we all tread the Homeward Path we will explore many Realms
And one day... we will all Realize that all experiences are Simply
Different ways in which The
All-That Is
Perceives Itself"

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Offlinemendo707homegrown
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Re: Ride along contam-fuzz ontop of rhizo mycelium [Re: Eclipse3130]
    #27655861 - 02/11/22 11:51 PM (2 years, 3 months ago)

Did you ever figure out what kind of contam is present in your first picture exactly? I know you guys are referencing it as piggy back mold but do you know the actual name of it? do you think it could have been a type of cobweb?

I recently lost hundreds of culture cups from not seeing the layer of white fuzz over top the myc when looking from above. Basically had made back up plates of every clean master culture i had one day and must of picked up the contam then. (narrowed it down to the building i had moved my lab to) then 2 days later before the contam was visually present i expanded out 100+ transfers plus LC and grain jars, and got completely destroyed a few days later when it finally grew in enough to see. Ive just wondered what the hell it was and ive been looking all over for an exact name but the closest thing i can find is cob web. strangely enough what ever contam it is never sporulated witch could have been an identifier. I kept a ton of the cultures to see what appears but still weeks later they just keep colonizing with fluff but no spores or color changes.

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