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OfflineWgdiplasito
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Mycelium turned red on agar plates
    #27436682 - 08/20/21 11:35 PM (2 years, 8 months ago)

Hello all, this is my first post here as I am new to the site, and the mushroom… growing… life!  So I started my process about a month and a half ago we hen I decided to convert my extra storage room into a dedicated clean room. I raised the floor, insulated everything, sheetrocked, muddled, and tiled everything, and built in a working area and an indoor greenhouse. I also built in a HEPA filter hood, and wired in electrical. Sanitized everything with 70% iso, and let a sanitizer lamp running for a day. Moving it around to get all of the room for a few hours each. I did a grow of Jedi Mind Fucks and just started with 6 jars, and just fished dunking them and they’re on their second flush… (First grow a success) !

So I wanted to try agar, I’ve done a lot of research about all this from start to finish and all the little extras but know I just don’t know anything yet so experiments are my only teacher. But as with everything I decide to do in my life, I learn and end up doing well with it. So I tried cutting some mycelium off of a brick that I started in BBC a jar after first flush before first dunk. I got charcoal infused agar to help with the colorant variation and easier viewing. But now I’ve noticed my agar has turned red where the mycelium was place. I’m sure it’s contaminated but you tell me?! If d as I, can I use the outreaches to try another plate or are these a lost cause?







Any tips would be great! Here’s some pics of my op!…


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Offlinesimonjester44
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Registered: 03/22/13
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Re: Mycelium turned red on agar plates [Re: Wgdiplasito] * 1
    #27438331 - 08/22/21 10:26 AM (2 years, 8 months ago)

Grats on your success.


I would say that it is contam.  I have seen it rip the pigment out of food coloring based agar tho.

It also looks like you may have some pin mold, but the resolution isnt great.

If you want to keep trying, take a small transfer from the end of that rhizo stuff thats in the clear.  Personally, I would abort this.  It honestly looks contaminated with several things, and that rizo seems like its just trying to outrun the bad.  It could probably be cleand up.


Other things:

Clone from a fruit next time.

Those fans don't look like they are capable of doing enough to push that filter.

Is the drywall mud painted?

In my first grow I built a Rube Goldberg machine, and what I learned was that I was better off with a SGFC and a SAB.


--------------------
"Never underestimate the power of friction as a construction adhesive."

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InvisibleSmartattack
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Registered: 12/21/18
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Re: Mycelium turned red on agar plates [Re: simonjester44]
    #27440011 - 08/23/21 07:00 PM (2 years, 8 months ago)

That's the color black agar turns during myc growth. Seen it a 100 times on my own plates.

That said, the plates also don't look healthy lol.


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Edited by Smartattack (08/23/21 07:02 PM)

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