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Offlinekarri0n
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Registered: 08/29/20
Posts: 698
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Re: Ms agar question [Re: shthd]
    #27443667 - 08/26/21 10:37 AM (2 years, 8 months ago)

That does sound like it could be explaining how they pour plates now that you mention it.

They mentioned that they used the print to make a mss syringe too though. And the germination is across the whole plate, so that really could be what's happening.

In any case, you want to put as little genetic material on the plate as possible to have the least chance of something that isn't the target organism to get in there.

If using a syringe, stretching one drop to several plates by streaking with a loop or sterile swab is a good way.

If using a print, lightly dabbing the print on one very small spot then streaking several plates is a good way.

Remember that any visible spores are actually hundreds to thousands, and that there are millions suspended in the water that you cannot see. You only need two.


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Invisiblec10h12n2o
serial dilutor
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Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: Ms agar question [Re: karri0n]
    #27444064 - 08/26/21 04:04 PM (2 years, 8 months ago)

Check out the "working with spores" section of my guide

https://www.shroomery.org/forums/showflat.php/Number/24330957/fpart/all/vc/1


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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OfflineTheBoJim
Strangest
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Registered: 07/23/20
Posts: 449
Last seen: 1 year, 2 months
Re: Ms agar question [Re: c10h12n2o]
    #27468664 - 09/14/21 08:56 PM (2 years, 7 months ago)

Quote:

c10h12n2o said:
Are you boiling your agar at all? You should really check out my agar guide in my sig

Like they said the agar isnt really the issue here. Thats more of a cosmetic thing.

Check this thread as well, it shows some examples of hidden contams

https://www.shroomery.org/forums/showflat.php/Number/22020260

Also, i just started a new project i will post in a few weeks (this thread and a few others gave me the idea). Basically i combined spores and clean myc with various contams to show what cultures look like when they are intermingled with various contams, should be pretty helpful for people.

Contams look different intermingled w cube myc vs on their own





Great thread. I found his last sentence very fitting to op.


Quote:

Mad Season said:


This right here is why I never say that I don't need to improve my techniques, or that I'm 100% clean. I'm not, and I'm constantly working on my sterile procedures.




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