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Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 9 months, 3 days
Interesting study: ozone sufficiently pasteruizes sawdust substrate
    #27429790 - 08/16/21 01:32 AM (2 years, 8 months ago)

I know this is a few years old, but I couldn't find it on this site with a search. It was found that pumping in ozone at 150 ppm or greater (30 minutes) was strong enough to (at least) pasteurize sawdust+bran+gypsum substrate for oyster mushroom growth. 100 ppm and lower was not sufficient. The highest concentrations of ozone caused the substrates to pin fastest, <my opinions start here> which could mean lower levels left more bio-burden for the mushrooms to overcome, or it could mean the high levels of ozone broke down nutrients to be more bio-available to the mycelia. I lean toward the first explanation, because the photos seem to indicate that the lowest effective ozone treatments started more quickly despite pinning last. I think some contaminants remained and grew, more so in the low treatment groups, so the final colonization was slower in the low ozone groups. The early colonization may have been slowed by residual ozone in the high treatment groups. One explanation of the slowing of the lower treatments is that that vegetative growth was killed but endospores were not. Or some tiny percent of (all) microbes survived. Or some type of microbe survived. We don't know based on this work.

I find this somewhat encouraging because my contingency plan (if I ever get an infection that can't be shaken) is to blast the area with ozone for a few hours.

A  New  Method  to  Sterilise  Mushroom  Substrate  for  Oyster
Mushroom Cultivation
http://www.conscientiabeam.com/ebooks/7-4thICETSR-506-2015-(45-51).pdf

Edited by gone-pear-shaped (08/16/21 02:41 AM)

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OfflinePsilosopherr
A psilly goose
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Registered: 02/15/12
Posts: 12,280
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Re: Interesting study: ozone sufficiently pasteruizes sawdust substrate [Re: gone-pear-shaped]
    #27429801 - 08/16/21 01:54 AM (2 years, 8 months ago)

how interesting, thank you

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InvisibleFiatFirmamentum
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Registered: 04/15/19
Posts: 59
Loc: Central EU
Re: Interesting study: ozone sufficiently pasteruizes sawdust substrate [Re: Psilosopherr]
    #27431140 - 08/17/21 04:05 AM (2 years, 8 months ago)

That's really interesting. 150ppm is less than g/m3 of O3.

Seemingly, cheap ($50) ozone generator claim to produce 20 grams of O3 per hour. It's seems likely, because 1 KWh generates ~144g of ozone in industrial settings.

Unfortunately, ozone is really toxic (toxic like "permanent lung damage", not "vomiting") in these concentrations and I can't imagine safely using it in apartment - but I can imagine safely using it in utility room with windows - by remotely controlling ventilation and ozone generator.

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Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 9 months, 3 days
Re: Interesting study: ozone sufficiently pasteruizes sawdust substrate [Re: FiatFirmamentum]
    #27431151 - 08/17/21 04:30 AM (2 years, 8 months ago)

I have a cheap one that says it produces 20 g per hour. (I consider it as 2g-20g per hour since the output may depend on some characteristic of the air input.) It scares me so I never use it, but I used to put it in a closed room with a towel blocking the space under the floor. If I needed to go in, I wore goggles and held my breath. It worked to deodorize laundry, but so does a tiny USB powered unit that's much safer.

I just found a cheap mid-powered unit with a hose output for use in water, or in an enclosed space like a substrate bag. I will use it under my range hood, which is perfectly safe.

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InvisibleWiscoregon
Harmonic Surfer


Registered: 11/08/11
Posts: 108
Re: Interesting study: ozone sufficiently pasteruizes sawdust substrate [Re: gone-pear-shaped]
    #27451113 - 08/31/21 02:58 PM (2 years, 8 months ago)

Thanks for sharing this. I have given some thought to using O3 as a means of pasteurizing or sterilizing and I find it useful for doing maintenance scrubs of work areas now and again.

One thought that came to mind for me, was that some countries (France for example) uses O3 as a substitute for chlorine for cleaning large municipal water supplies.  Maybe a sub could be immersed in water then the water ozone treated and finally drained to the desired moisture content and could perhaps be more effective (maybe less?) than treating a substrate directly with gas. 

Cool share, thanks.

Edited by Wiscoregon (08/31/21 03:00 PM)

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OfflineOra
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Registered: 07/09/21
Posts: 734
Loc: in your heart
Last seen: 2 years, 5 months
Re: Interesting study: ozone sufficiently pasteruizes sawdust substrate [Re: Wiscoregon]
    #27451282 - 08/31/21 05:37 PM (2 years, 8 months ago)

you should never breath straight ozone, but its cool to ozone rooms in your house as long as you close the door. i ozone my closet before doing SAB work. give it 2 hours for the ozone to break back into oxygen, then go in.

i used to drink ozonated water until my machine broke. its really healthy. they do ozone IV's in functional medicine clinics and also prolozone therapy where they inject ozone gas into an area of the body where you have injury or pain and it speeds healing by an incredible amount.

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OfflineJessesun
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Registered: 09/21/21
Posts: 14
Loc: China Flag
Last seen: 7 months, 22 days
Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">有趣的研究:臭氧充分消毒锯末基质</font></font> [Re: gone-pear-shaped]
    #27478988 - 09/23/21 01:13 AM (2 years, 7 months ago)

Quote:

gone-pear-shaped said:
I know this is a few years old, but I couldn't find it on this site with a search. It was found that pumping in ozone at 150 ppm or greater (30 minutes) was strong enough to (at least) pasteurize sawdust+bran+gypsum substrate for oyster mushroom growth. 100 ppm and lower was not sufficient. The highest concentrations of ozone caused the substrates to pin fastest, <my opinions start here> which could mean lower levels left more bio-burden for the mushrooms to overcome, or it could mean the high levels of ozone broke down nutrients to be more bio-available to the mycelia. I lean toward the first explanation, because the photos seem to indicate that the lowest effective ozone treatments started more quickly despite pinning last. I think some contaminants remained and grew, more so in the low treatment groups, so the final colonization was slower in the low ozone groups. The early colonization may have been slowed by residual ozone in the high treatment groups. One explanation of the slowing of the lower treatments is that that vegetative growth was killed but endospores were not. Or some tiny percent of (all) microbes survived. Or some type of microbe survived. We don't know based on this work.

I find this somewhat encouraging because my contingency plan (if I ever get an infection that can't be shaken) is to blast the area with ozone for a few hours.

A  New  Method  to  Sterilise  Mushroom  Substrate  for  Oyster
Mushroom Cultivation
http://www.conscientiabeam.com/ebooks/7-4thICETSR-506-2015-(45-51).pdf




When you have ozone to disinfect, you need to what the CT value is?
C=ozone concentration
T=time

(in air, 1ppm=2.14mg/m3, in water, 1ppm=1.0g/m3)

and that it has big difference in ozone application in air and water. Like 150ppm ozone concentration in air for indoors cultivation of edible fungi, what ozone system do you need?

Ozone output per hour=CO3*Volume/(1-61%)x75%, not think of fresh air volume

CO3 is concentration of ozone
Volume is space
61% is ozone hour decay rate
75% is working efficiency of ozone generator

1,you also need to consider if you need to control CO3 by PLC automatically?
2,ozone tail gas need to be treated with (0.1ppm, 8hours is US ozone standard)


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