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InvisibleCogito Ergo Sum
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Brown rice and rye spawn jars
    #27428922 - 08/15/21 09:00 AM (3 years, 5 months ago)

I'm currently in the middle of trying to make a couple of spawn jars with rye. I had an issue with brown rice and decided to try a different medium to help me with getting a spawn going.

Basically, I followed the broke boi tek on youtube to a t (shown here:
and after three months of waiting the only thing that showed up in my jars was just a tiny speck of mold. I used a super charged spore syringe and made sure my work area was as clean as possible. Out of the four jars, only one showed mold (at least I got the sterilization down I guess).

Anyways, I wanted to ask if anyone has had success with brown rice and if so what did you follow exactly? Since I had no success with the previous method I'm currently trying the soak and PC method with rye grains as explained in "The Psilocybin Mushroom Bible". I wanted to give it another shot with brown rice since I'm already in the process of (attempting to) spawn again but I've seen so many ancient threads with mixed info here on the subject I figured I'd start another.

I only have 10ml of the solution left. It's hard for me to get ahold of due to reasons and I'm really hoping to get something going one way or another. I'm open to any tips or suggestions. Thanks in advance, guys.

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InvisibleStipe-n CapMDiscord
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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27428930 - 08/15/21 09:10 AM (3 years, 5 months ago)

If you're going to insist upon using septic starting materials like spore solution I'd recommend sticking to BRF...agar is obviously the way to go but if you really like spore syringes for some reason use brf.

Injecting spores onto any grains regardless of prep method you're going to run into problems. You could be working at the CDC in a secure lab and still contaminate your jars because the solution itself carries contaminants, your environment is irrelevant at this point.

Also, stop watching that shit, those people are fucking retarded.

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InvisibleCogito Ergo Sum
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Re: Brown rice and rye spawn jars [Re: Stipe-n Cap]
    #27428934 - 08/15/21 09:16 AM (3 years, 5 months ago)

I'm just trying to get started, tbh. Once I have a few yields I plan on experimenting with different techniques. Agar will be next but it's hard for me to come by the materials I need.
Basically the problem I had was that nothing grew at all. I'm not sure if the grains were too dry from sterilization or what, but like I said the only thing that grew was the tiniest bit of mold in ONE jar out of four...

I'm really hoping to get at least one yield so I can grab a few spore prints and save them for future methods. Like I said, I'm just trying to start but so far I haven't had any luck.
Not gonna give up though.

Edit: Also, I know they're retarded but I'm not trying to set up a full blown lab yet. I'm just working with limited materials and just trying to get a good flush or two.

Edited by Cogito Ergo Sum (08/15/21 09:19 AM)

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Invisiblecoversall
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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum] * 1
    #27428944 - 08/15/21 09:23 AM (3 years, 5 months ago)

Quote:

Cogito Ergo Sum said:
I'm just trying to get started, tbh. Once I have a few yields I plan on experimenting with different techniques. Agar will be next but it's hard for me to come by the materials I need.
Basically the problem I had was that nothing grew at all. I'm not sure if the grains were too dry from sterilization or what, but like I said the only thing that grew was the tiniest bit of mold in ONE jar out of four...

I'm really hoping to get at least one yield so I can grab a few spore prints and save them for future methods. Like I said, I'm just trying to start but so far I haven't had any luck.
Not gonna give up though.

Edit: Also, I know they're retarded but I'm not trying to set up a full blown lab yet. I'm just working with limited materials and just trying to get a good flush or two.




If you want to get some mush, and some prints, without a lab or whatever, just run the PF tek. It's basically fool proof and can yield amazing results.


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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InvisibleStipe-n CapMDiscord
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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum] * 1
    #27428958 - 08/15/21 09:34 AM (3 years, 5 months ago)

:whathesaid:

That's why I suggested brf.

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Invisiblecoversall
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Re: Brown rice and rye spawn jars [Re: Stipe-n Cap]
    #27428971 - 08/15/21 09:41 AM (3 years, 5 months ago)

Quote:

p9hu7 said:
:whathesaid:

That's why I suggested brf.




I feel like the urge to wave the BRF cake flag so hard. It is a great tek but so many people seem to want to shun it! ;_;


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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InvisibleStipe-n CapMDiscord
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Re: Brown rice and rye spawn jars [Re: coversall]
    #27428972 - 08/15/21 09:43 AM (3 years, 5 months ago)

It's definitely way better than these new reddit and YouTube deals. This is the tried and true beginners method.

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InvisibleCogito Ergo Sum
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Re: Brown rice and rye spawn jars [Re: coversall]
    #27428977 - 08/15/21 09:45 AM (3 years, 5 months ago)

Thanks guys, I'll give that a try. I wanted to spawn from a grain then transfer to a bulk growth medium but I don't mind trying the pf tek just to get started. Even if it only yielded a few I'd be happy at this point just to get a print to go further.

After reading about how "foolproof" growing is from so many people here I kinda got discouraged to be honest. I'm determined to get it right though. Thanks for the recommendations. Heading to the store to get the materials for that now...

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Invisiblecoversall
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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27428981 - 08/15/21 09:50 AM (3 years, 5 months ago)

Quote:

Cogito Ergo Sum said:
Thanks guys, I'll give that a try. I wanted to spawn from a grain then transfer to a bulk growth medium but I don't mind trying the pf tek just to get started. Even if it only yielded a few I'd be happy at this point just to get a print to go further.

After reading about how "foolproof" growing is from so many people here I kinda got discouraged to be honest. I'm determined to get it right though. Thanks for the recommendations. Heading to the store to get the materials for that now...




Honestly to make things fool proof, pick a respected tek thread, switch off your brain (take this with a pinch of salt), and just do what the thread tells you to do.

Make a promise to youtself that you will follow one tek for each grow. The urge to tinker when you are starting out will complicate things and muddy the water.

Read this, do what it says, grow mushrooms!


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

Edited by coversall (08/15/21 09:51 AM)

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InvisibleCogito Ergo Sum
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Re: Brown rice and rye spawn jars [Re: coversall]
    #27428990 - 08/15/21 09:59 AM (3 years, 5 months ago)

Quote:

Honestly to make things fool proof, pick a respected tek thread, switch off your brain (take this with a pinch of salt), and just do what the thread tells you to do.

Make a promise to youtself that you will follow one tek for each grow. The urge to tinker when you are starting out will complicate things and muddy the water.

Read this, do what it says, grow mushrooms!




Thanks, bro. I pretty much have everything I need except the perlite and I'm on my way to grab that at the moment. I'll start a thread chronicling the progress as it goes. I really appreciate you guys knowledge. I'm a total noob to this...

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Invisiblecoversall
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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27429001 - 08/15/21 10:10 AM (3 years, 5 months ago)

Here I am talking about keeping things simple, and I'm going to throw you a curve ball.

I would hold off on buying stuff for fruiting for now.

Make the cakes, inoculate them, and get them growing.

Then maybe read and ask about the various ways of fruiting BRF cakes! imo there are better options than the shotgun fruiting chamber.


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27429014 - 08/15/21 10:20 AM (3 years, 5 months ago)

Quote:

Cogito Ergo Sum said:
Thanks guys, I'll give that a try. I wanted to spawn from a grain then transfer to a bulk growth medium but I don't mind trying the pf tek just to get started. Even if it only yielded a few I'd be happy at this point just to get a print to go further.

After reading about how "foolproof" growing is from so many people here I kinda got discouraged to be honest. I'm determined to get it right though. Thanks for the recommendations. Heading to the store to get the materials for that now...




Hey Cognito Ergo, do not give up on multi-spore syringes as a way to get genetics started.  Yes you can squirt it on Rice or grain directly.  Yes you "Might" see contamination.  With Jars that colonize without contam', spawn them.  You "Might" still see contamination later. 
Your goal is to harvest the healthiest most vigorous mushrum and clone it with a needle and sugar water, or LME water 4%.  Tear it in half to sample only sterile tissue.

You can also squirt Multi-spore syringes directly to Sugar Water, and yes, you "Might" see contamination later that hides inside.  You must test it on Agar!


If you have no Still Air Box or HEPA Filtered Air, use the 250F Oven Method with a Wire Rack over hanging the cracked open door.  I pour and inoculate Agar this way and have only had one contaminated dish over 30 dishes poured and inoculated this way.

Edited by MushBrain1 (08/15/21 10:25 AM)

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Invisiblecoversall
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Re: Brown rice and rye spawn jars [Re: MushBrain1]
    #27429022 - 08/15/21 10:27 AM (3 years, 5 months ago)

Quote:

MushBrain1 said:
Quote:

Cogito Ergo Sum said:
Thanks guys, I'll give that a try. I wanted to spawn from a grain then transfer to a bulk growth medium but I don't mind trying the pf tek just to get started. Even if it only yielded a few I'd be happy at this point just to get a print to go further.

After reading about how "foolproof" growing is from so many people here I kinda got discouraged to be honest. I'm determined to get it right though. Thanks for the recommendations. Heading to the store to get the materials for that now...




Hey Cognito Ergo, do not give up on multi-spore syringes as a way to get genetics started.  Yes you can squirt it on Rice or grain directly.  Yes you "Might" see contamination.  With Jars that colonize without contam', spawn them.  You "Might" still see contamination later. 
Your goal is to harvest the healthiest most vigorous mushrum and clone it with a needle and sugar water, or LME water 4%.  Tear it in half to sample only sterile tissue.

If you have no Still Air Box or HEPA Filtered Air, use the 250F Oven Method with a Wire Rack over hanging the cracked open door.  I pour and inoculate Agar this way and have only had one contaminated dish over 30 dishes poured and inoculated this way.




Hey Mushbrain! Forgive me, but spore to grain, and working in the oven isn't great advice. An SAB is very easy to make and will be a much better place to work than the oven.


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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Re: Brown rice and rye spawn jars [Re: coversall] * 1
    #27429124 - 08/15/21 12:11 PM (3 years, 5 months ago)

Kneeling in front of a hot af oven to try and do sterile work sounds so uncomfortable :lol:  the only thing I’d even consider possibly suggesting using an oven for is dehydrating mush and even that gives me apprehension.

Reminds me of that pic of that dude in a tyvek suit inoculating pf jars in front of his oven

Faht

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InvisibleCogito Ergo Sum
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Re: Brown rice and rye spawn jars [Re: MushBrain1]
    #27429461 - 08/15/21 05:40 PM (3 years, 5 months ago)

I have a SAB that I work with, I think that's why I had limited contamination with my last batch. The problem is, I don't have a legit pressure cooker. I only have an instant pot and I've never used it to sterilize. I've just cooked them on the stove for about 3 hours with a tight lid on low heat. Initially I thought that was my problem. Now I'm concerned that I overcooked the grains and they dried out too much...

In any case, I'm going with the PF tek next. I now have everything I need to go through with it, so fingers crossed, I'll have some luck.

Thank you all for the recommendations. I'm taking them to heart and will attempt to use them to get this started!

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InvisibleCogito Ergo Sum
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Re: Brown rice and rye spawn jars [Re: coversall]
    #27429464 - 08/15/21 05:43 PM (3 years, 5 months ago)

Quote:

imo there are better options than the shotgun fruiting chamber.




What would you recommend checking out? I've only seen the shotgun fruiting chamber and what phillygoldenteacher recommended in the above youtube video. Basically a shoebox tote with vermiculite and gypsum on a plastic bag with plenty of moisture and heat.

I'm open to suggestions and I hope to reach the point where I need to ask this question. Fingers crossed I'll see some pins soon...

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Invisiblecoversall
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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27429870 - 08/16/21 04:40 AM (3 years, 4 months ago)

Quote:

Cogito Ergo Sum said:
Quote:

imo there are better options than the shotgun fruiting chamber.




What would you recommend checking out? I've only seen the shotgun fruiting chamber and what phillygoldenteacher recommended in the above youtube video. Basically a shoebox tote with vermiculite and gypsum on a plastic bag with plenty of moisture and heat.

I'm open to suggestions and I hope to reach the point where I need to ask this question. Fingers crossed I'll see some pins soon...




No idea who phillygoldenteacher is.

Check out some of the simple shoebox threads on this forum for the best adivce
Some good ones off the top of my head:
Munch's thread
Shaper's thread
The BRF as spawn thread
Otherwise theres the humidty chamber FC


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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InvisibleCogito Ergo Sum
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Re: Brown rice and rye spawn jars [Re: MushBrain1]
    #27431996 - 08/17/21 05:26 PM (3 years, 4 months ago)

Well, here I am two days later and fingers crossed, I now have five half pint pf tek jars that I inoculated with a spore syringe. I hope it takes this time because I used up the last of what I had.
Thanks again for all the advice. Hopefully I'll be posting some spawned jars here in a few weeks or so!

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Invisiblecoversall
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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27432630 - 08/18/21 02:15 AM (3 years, 4 months ago)

Quote:

Cogito Ergo Sum said:
Well, here I am two days later and fingers crossed, I now have five half pint pf tek jars that I inoculated with a spore syringe. I hope it takes this time because I used up the last of what I had.
Thanks again for all the advice. Hopefully I'll be posting some spawned jars here in a few weeks or so!




Keep in mind that one of the bad things about spores to BRF cakes is that it can be a slow process. My first run of spores to PF tek took around 28 days to fully colonise.


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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InvisibleCogito Ergo Sum
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Re: Brown rice and rye spawn jars [Re: coversall]
    #27433310 - 08/18/21 04:00 PM (3 years, 4 months ago)

Damn, didn't realize it could take that long...
I was a bit concerned about the moisture content, seemed a bit dry. Although I followed the steps exactly as said, so I hope all's well.
I'll keep an update in about a month or so whenever they start to colonize. Hopefully I'll at least see some mycelial growth before then to indicate they're good. My last attempt was a total failure.

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InvisibleCogito Ergo Sum
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Re: Brown rice and rye spawn jars [Re: coversall]
    #27452613 - 09/01/21 05:55 PM (3 years, 4 months ago)

Just wanted to thank you all again and also update in case you guys were interested. The PF cakes you guys suggested are coming up swimmingly and I couldn't be happier! I inoculated two grain jars just as an experiment to see if I would get any results this time (using rye berries) and unfortunately, no such luck.
However, all five of the BRF and vermiculite jars are showing healthy amounts of rhizomorphic growth!
Thank you guys so much for the recommendations and I'll continue posting my results as they come to me.
You all rock.

Also, please forgive me if I break any forum rules. I think I read them over pretty well but I'm still pretty new to these forums. Hopefully you guys can see the images below ok...






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InvisibleCogito Ergo Sum
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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27452650 - 09/01/21 06:38 PM (3 years, 4 months ago)







Reposting after hosting on this site. Just trying to figure out how to post pics on this site. Apologies...

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OfflineMushBrain1
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Re: Brown rice and rye spawn jars [Re: fahtster]
    #27457425 - 09/05/21 05:06 PM (3 years, 4 months ago)

Quote:

fahtster said:
Kneeling in front of a hot af oven to try and do sterile work sounds so uncomfortable :lol:  the only thing I’d even consider possibly suggesting using an oven for is dehydrating mush and even that gives me apprehension.

Reminds me of that pic of that dude in a tyvek suit inoculating pf jars in front of his oven

Faht




Well the proof the Cracked Oven Method rocks is in only 1 bad Agar Dish poured above 250F cracked Oven Door on over-hanging wire Rack.  It defies logic.  Should be shit loads of contam's, yet there are very few!

I have a Still Air Box and use it also.  I wait until my Fridge shuts off for real Still Air, and Wear an N95 Mask.  I do not bother with Cracked Oven Method.  It rocks!  Yes I want a HEPA Flow Box and have zero space for that.

Yes I admit spore syringe to grain or liquid cuture is a poor practice, but I did not know that and succeeded out of ignorance!

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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27457530 - 09/05/21 06:40 PM (3 years, 4 months ago)

Quote:

Cogito Ergo Sum said: I only have an instant pot and I've never used it to sterilize. I've just cooked them on the stove for about 3 hours with a tight lid on low heat. Initially I thought that was my problem. Now I'm concerned that I overcooked the grains and they dried out too much...




Instantpot is a far better option than steaming in a pot. Most models do get to 15psi intially and if you cancel the cook and restart they should get to 15+psi again and then back off down again. I do not bother doing restarts most times, just set to the max time. There is no babysitting them so it makes no sense for you to use the stove -unless your jars do not fit of course. Some newer IPs do go to higher pressure for the full cook time.

If you insist on using MS syringes direct then use very little. I get 25 drops per ml, so 250 in a 10ml syringe. If there are 10 contams in your syringe and you inject 1cc then there is a good chance one got in, and many contams will outrun the cube myc. If you inject a single drop it typically takes a lot longer to intially grow, but this does not matter too much if you injected no contams with it.

You an weigh your jars and hydrated grains to monitor any moisture loss.

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Re: Brown rice and rye spawn jars [Re: blackout] * 1
    #27457727 - 09/05/21 10:21 PM (3 years, 4 months ago)



The first picture is 4 14 gm mason jars. The 2nd is more than that. All from BRF, an instapot and homemade spore syringes.
Listen to what the guys who are making sense say and ignore the others. Everyone has an opinion and that doesn’t help sometimes. The amount of time you’ve spent experimenting has cost you actually producing something.
BRF is by its nature inefficient but it’s also easy. That’s why it’s been around since the 1990’s.
And that’s  why people who just want to have some mushrooms still use it. It works well, not to peak efficiency.
You’ll get some mushrooms, you’ll get contamination, you may get no growth in some jars at all.
Every grow will get better. Then move on if you want to. But you’ll have mushrooms and you’ll have a better idea of what someone is trying to tell you. Basically build a better bullshit meter.
I’ve had to slow down. Started 7 jars every 2 weeks, then moved to every 4 weeks.  I like watching the grows but I have more than enough.
Spore syringes are a complete crap shoot but that doesn’t matter to me. I have more than I need for my purposes and I can grow more, albeit inefficiently, any time I want.

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Re: Brown rice and rye spawn jars [Re: The Tao]
    #27460692 - 09/08/21 04:41 PM (3 years, 4 months ago)

Quote:

The Tao said:

Listen to what the guys who are making sense say and ignore the others. Everyone has an opinion and that doesn’t help sometimes. The amount of time you’ve spent experimenting has cost you actually producing something.




That's why I'm picking everyone's brains here. The cakes are coming along nicely as of today. No sign of contamn and they're about 60% colonized at this point (I screwed up and ground up the rice too fine and it's super compacted within the jars, but still colonizing well).
I think my next step is going to be a BRF to bulk monotub by shredding the cake with a cheese grater into a coir substrate. Which brings me to my next question.
Has anyone messed around with a coir vs CVG substrate and had any noticeably different results? I was thinking of going with just coir, but after reading a few teks it seems it may be beneficial to mix a bit of gypsum and vermiculite into it. I was wondering if you guys had any comments to add to that decision as far as ratios go since I'm a first time grower in an area with very high humidity.
In any case, thank you guys for all the input, I am weighing every option I have as I go.
My next step is to take a few good spore prints to mess around with agar next.
Fingers crossed!

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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27460699 - 09/08/21 04:46 PM (3 years, 4 months ago)

I'll probably try that next. I first used quart sized jars that wouldn't fit into the instant pot, but since going to BRF cakes in pint sized jars, they'll definitely fit into the pressure cooker. In any case, I haven't had any luck with syringe to grain inoculation. This is the third time I've tried, modifying the preparation methods, and still no sign of growth in those containers. However, I'm extremely happy that my BRF cakes are showing healthy growth. I have a bit of anxiety as far as fruiting them goes, I'm planning on working with a shoebox monotub as of now.
As long as I can harvest a few healthy caps for spores to continue work I'll be happy for the moment...

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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27460706 - 09/08/21 04:53 PM (3 years, 4 months ago)

lol anyones pasta consumption increased after they started mush cult?

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Re: Brown rice and rye spawn jars [Re: Cogito Ergo Sum]
    #27460794 - 09/08/21 06:02 PM (3 years, 4 months ago)

Quote:

Cogito Ergo Sum said:
Quote:

The Tao said:

Listen to what the guys who are making sense say and ignore the others. Everyone has an opinion and that doesn’t help sometimes. The amount of time you’ve spent experimenting has cost you actually producing something.




That's why I'm picking everyone's brains here. The cakes are coming along nicely as of today. No sign of contamn and they're about 60% colonized at this point (I screwed up and ground up the rice too fine and it's super compacted within the jars, but still colonizing well).
I think my next step is going to be a BRF to bulk monotub by shredding the cake with a cheese grater into a coir substrate. Which brings me to my next question.
Has anyone messed around with a coir vs CVG substrate and had any noticeably different results? I was thinking of going with just coir, but after reading a few teks it seems it may be beneficial to mix a bit of gypsum and vermiculite into it. I was wondering if you guys had any comments to add to that decision as far as ratios go since I'm a first time grower in an area with very high humidity.
In any case, thank you guys for all the input, I am weighing every option I have as I go.
My next step is to take a few good spore prints to mess around with agar next.
Fingers crossed!



https://www.shroomery.org/forums/showflat.php/Number/24461957
Proper spore printing. Good read. Answers all the questions you didn’t know you had.

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