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InvisibleCilla_Sihbin
Alpha Frau
Female


Registered: 07/05/21
Posts: 49
Loc: both sides of the Atlanti...
Where to buy Gentamicin ?
    #27419849 - 08/08/21 02:38 PM (2 years, 9 months ago)

Hi there,

Liberty Cap season will soon be with us.
I've talked my mum into letting me grow mushrooms
in the front garden.  (I think she's imagining
button mushrooms on the lawn)

So the plan is to collect lots of liberty caps,
create spore prints, make agar plates with gentamicin.
And probably make an awful mess of everything and
get it wrong, but practice makes perfect, or in my case,
practice makes less awful than the time before.

Where can I purchase gentamicin, small amount,
0.25g or 0.5g or 1.0g inexpensively, in the UK or Europe
.... or planet Earth generally.

Thanks to anyone/everyone that offers hints/tips/solutions.

Love and Kisses

Cilla :-) x x x


--------------------
I believe in freedom of speech.  Real freedom of expression.
Send me an encrypted message, on absolutely any subject you like, whether politically correct or politically incorrect. Whether it's about you, a politician, the world or space aliens. Provided it's encrypted, I promise that I will never-ever share the contents of your message with anyone else.  So, send me an encrypted message, why don't'cha? :-) x x x

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Invisibleseldom seen
April Fool
Male User Gallery


Registered: 11/03/07
Posts: 1,039
Re: Where to buy Gentamicin ? [Re: Cilla_Sihbin]
    #27420747 - 08/09/21 09:36 AM (2 years, 9 months ago)

Idk about the antibiotics but check out what Baba Yaga did w libs outdoors...

https://www.shroomery.org/forums/showflat.php?Cat=&Board=30&Number=26974755&PHPSESSID=&fpart=1#26974755


--------------------

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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Where to buy Gentamicin ? [Re: seldom seen]
    #27420949 - 08/09/21 12:26 PM (2 years, 9 months ago)

You don't need antibiotics to clone or grow from wild mushrooms. Just technique


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InvisibleCilla_Sihbin
Alpha Frau
Female


Registered: 07/05/21
Posts: 49
Loc: both sides of the Atlanti...
Re: Where to buy Gentamicin ? [Re: seldom seen] * 1
    #27421543 - 08/09/21 06:28 PM (2 years, 9 months ago)

Quote:

seldom seen said:
Idk about the antibiotics but check out what Baba Yaga did w libs outdoors...

https://www.shroomery.org/forums/showflat.php?Cat=&Board=30&Number=26974755&PHPSESSID=&fpart=1#26974755




Hi Seldom Seen,
Thank you for posting that link.
Baba Yaga is my inspiration (for this libs project) 
He's my reason for thinking it might be possible.
Other people say that trying to cultivate libs is a waste of time.

I messaged him recently. 
He swears that his rainbow isn't urine (in his profile pic).
He says it's a real rainbow.
When i asked: How did you get it in there ?
He didn't answer.  He's clearly keeping secrets from us ;-)

Are you also considering a 'libs project' Seldom Seen ?
If so, will you be doing a journal about it ?
If you do, I would like to tune into your journal. 
(Maybe i should keep a journal too)

Love and kisses

Cilla :-)  x x x


--------------------
I believe in freedom of speech.  Real freedom of expression.
Send me an encrypted message, on absolutely any subject you like, whether politically correct or politically incorrect. Whether it's about you, a politician, the world or space aliens. Provided it's encrypted, I promise that I will never-ever share the contents of your message with anyone else.  So, send me an encrypted message, why don't'cha? :-) x x x

Extras: Filter Print Post Top
InvisibleCilla_Sihbin
Alpha Frau
Female


Registered: 07/05/21
Posts: 49
Loc: both sides of the Atlanti...
Re: Where to buy Gentamicin ? [Re: bodhisatta]
    #27421560 - 08/09/21 06:39 PM (2 years, 9 months ago)

Quote:

bodhisatta said:
You don't need antibiotics to clone or grow from wild mushrooms. Just technique




hi Bodhisatta,

I'm a newbie, I am destined to get it wrong and have failures.
(Because we learn by making mistakes, that is, if the mistakes
don't discourage us to the point of giving up)
Technique probably is the key detail, but I am not there yet.

So I see myself as cheating, a little bit, by killing off the
bacteria with gentamicin.  I'm just following the cultivation
advice for wild strains.  I'm quite looking forward to playing
with agar dishes and agar media and making spore prints.
Hey maybe I should practice with supermarket mushrooms first.

Is a Bodhisatta the same as a Bodhisattva ?
And are you a Buddhist ?

Love and kisses

Cilla :-) x x x


--------------------
I believe in freedom of speech.  Real freedom of expression.
Send me an encrypted message, on absolutely any subject you like, whether politically correct or politically incorrect. Whether it's about you, a politician, the world or space aliens. Provided it's encrypted, I promise that I will never-ever share the contents of your message with anyone else.  So, send me an encrypted message, why don't'cha? :-) x x x

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Invisiblec10h12n2o
serial dilutor
 User Gallery


Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: Where to buy Gentamicin ? [Re: bodhisatta]
    #27421863 - 08/09/21 10:13 PM (2 years, 9 months ago)

Quote:

Cilla_Sihbin said:
Hi there,

Liberty Cap season will soon be with us.
I've talked my mum into letting me grow mushrooms
in the front garden.  (I think she's imagining
button mushrooms on the lawn)

So the plan is to collect lots of liberty caps,
create spore prints, make agar plates with gentamicin.
And probably make an awful mess of everything and
get it wrong, but practice makes perfect, or in my case,
practice makes less awful than the time before.

Where can I purchase gentamicin, small amount,
0.25g or 0.5g or 1.0g inexpensively, in the UK or Europe
.... or planet Earth generally.

Thanks to anyone/everyone that offers hints/tips/solutions.

Love and Kisses

Cilla :-) x x x




pretty much any lab supply store will either have it in stock or can order it for you. i cant recommend highly enough finding a local lab supply store and making friends with the staff :wink:

BUT


Quote:

bodhisatta said:
You don't need antibiotics to clone or grow from wild mushrooms. Just technique




this

there really isnt much advantage to using antibiotics for something like this. i havent used them for many years

for an outdoor grow, bacteria shouldnt be much of an issue anyway, fresh outdoor air is magic. and antibiotics arent going to help you with the stuff that would be a big issue before you spawn (stuff that actually parasitizes the myc, like mold)

if you want to use spores, just do serial streaks like in my agar guide and choose colonies from the later zones, they should have very few colonies of myc and be less intermingled with contams

for cloning, just use good technique and be sure to tear (not cut) the stipe and take tissue samples from inside the stipe

Baba's grow was so sick!!! id love to play with these sometime


--------------------

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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Invisibleseldom seen
April Fool
Male User Gallery


Registered: 11/03/07
Posts: 1,039
Re: Where to buy Gentamicin ? [Re: Cilla_Sihbin]
    #27422285 - 08/10/21 08:49 AM (2 years, 9 months ago)

Quote:

Cilla_Sihbin said:
Quote:

seldom seen said:
Idk about the antibiotics but check out what Baba Yaga did w libs outdoors...

https://www.shroomery.org/forums/showflat.php?Cat=&Board=30&Number=26974755&PHPSESSID=&fpart=1#26974755




Hi Seldom Seen,
Thank you for posting that link.
Baba Yaga is my inspiration (for this libs project) 
He's my reason for thinking it might be possible.
Other people say that trying to cultivate libs is a waste of time.

I messaged him recently. 
He swears that his rainbow isn't urine (in his profile pic).
He says it's a real rainbow.
When i asked: How did you get it in there ?
He didn't answer.  He's clearly keeping secrets from us ;-)

Are you also considering a 'libs project' Seldom Seen ?
If so, will you be doing a journal about it ?
If you do, I would like to tune into your journal. 
(Maybe i should keep a journal too)

Love and kisses

Cilla :-)  x x x





Hi Cilla, you're welcome!  I couldn't believe that grow when I first read through the thread, pretty fkn spectacular...I would follow that to the T and wish you the best of luck with it.  It's something I'd like to do eventually, but I have a couple other projects I want to get working on first.  I'd love to watch your project unfold if you keep a journal, keep us posted!  And I plan on keeping one for my fall projects and would love any feedback when the time comes. 

And listen to these guys about the agar approach, there's much you can do before you have to resort to antibiotics.  Maybe that's what Baba's rainbow is about lol.  Best of luck to you with this:mushroom2:


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OfflineSerpinfold
Stranger
Registered: 08/09/21
Posts: 23
Last seen: 1 year, 2 months
Re: Where to buy Gentamicin ? [Re: seldom seen]
    #27422354 - 08/10/21 09:56 AM (2 years, 9 months ago)

Honestly OP potato dextrose agar is fairly selective for fungi. You're unlikely to get a bacterial contamination on PDA. I've gotten a cloud of bloody moulds, al the colours of the rainbow, but never a bacterial colony. As long as you practive good sterile technique, you really don't need that gentamycin.

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