|
The Fresh Prints
Smell ya later



Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
|
Re: Psilocybe natalensis [Re: mycosavant] 1
#27415874 - 08/05/21 06:49 PM (2 years, 6 months ago) |
|
|
Quote:
mycosavant said:
Very nice! I definitely concur with A.k.a. on that being a solid culture. What's it look like on agar?

This is the culture I used. T1 from Crack's print
--------------------

|
Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,401
Loc: Over by your Mama's house
Last seen: 1 day, 2 hours
|
|
|
Lostkeys
Fractal Thought Pattern



Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 months, 23 days
|
|
Quote:
The Fresh Prints said:
Quote:
mycosavant said:
Very nice! I definitely concur with A.k.a. on that being a solid culture. What's it look like on agar?

This is the culture I used. T1 from Crack's print
I have a few plates that look like that. Got the spores from a guy on reddit.
|
gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 6 months, 4 days
|
Re: Psilocybe natalensis [Re: Lostkeys]
#27416236 - 08/06/21 12:34 AM (2 years, 6 months ago) |
|
|
I've been reading some of Violet's teks recently, and perhaps her strategies would be useful for this species, since its phenotypes are so variable. I only grew out two strains, but one was is twice as fast as the other. That's not to mention the issues of overlay or variable fruit size.
Violet uses petri dishes very briefly, only to get clean seeming growth*, then grows on grass seed (or brown rice) to rank strains based on colonization speed, pinning characteristics, and other traits with a faster turnaround than with normal methods. I've always wanted to grow ten strains of X** and compare them, but I can't manage that many jars and tubs. I end up with overgrown neglected petri dishes. https://www.shroomery.org/forums/showflat.php/Number/24856009
* Violet doesn't mention jar inoculation with hole punch, but I think that would be ideal so they all have uniform inoculum. **X is anything with difficult phenotypes: natalensis or penis envy.
|
Lostkeys
Fractal Thought Pattern



Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 months, 23 days
|
|
Quote:
gone-pear-shaped said: I've been reading some of Violet's teks recently, and perhaps her strategies would be useful for this species, since its phenotypes are so variable. I only grew out two strains, but one was is twice as fast as the other. That's not to mention the issues of overlay or variable fruit size.
Violet uses petri dishes very briefly, only to get clean seeming growth*, then grows on grass seed (or brown rice) to rank strains based on colonization speed, pinning characteristics, and other traits with a faster turnaround than with normal methods. I've always wanted to grow ten strains of X** and compare them, but I can't manage that many jars and tubs. I end up with overgrown neglected petri dishes. https://www.shroomery.org/forums/showflat.php/Number/24856009
* Violet doesn't mention jar inoculation with hole punch, but I think that would be ideal so they all have uniform inoculum. **X is anything with difficult phenotypes: natalensis or penis envy.
I sort of used a variation of her in vitro method, except using brf mix per bodhisatta. It isn't colonizing very fast though.
-------------------- So heavy I fell through the earth...

|
nosf3r4tu


Registered: 03/26/19
Posts: 775
|
|
Quote:
gone-pear-shaped said: perhaps her strategies would be useful for this species, since its phenotypes are so variable. I only grew out two strains, but one was is twice as fast as the other. That's not to mention the issues of overlay or variable fruit size.
* Violet doesn't mention jar inoculation with hole punch, but I think that would be ideal so they all have uniform inoculum. **X is anything with difficult phenotypes: natalensis or penis envy.
What you see are not phenotypes. It's environmental. Just different fruiting conditions. (ie : different spawn rate/nutrients available, humidity, temperature, FAE,etc...) the exact same, culture will behave differently under different circumstance.
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 2 hours, 4 minutes
|
Re: Psilocybe natalensis [Re: nosf3r4tu]
#27416623 - 08/06/21 09:30 AM (2 years, 6 months ago) |
|
|
I think this is the best, most normal natal tub I’ve ever had 
--------------------
LAGM2020     
|
gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 6 months, 4 days
|
Re: Psilocybe natalensis [Re: nosf3r4tu]
#27416631 - 08/06/21 09:42 AM (2 years, 6 months ago) |
|
|
Quote:
nosf3r4tu said:
Quote:
gone-pear-shaped said: perhaps her strategies would be useful for this species, since its phenotypes are so variable. I only grew out two strains, but one was is twice as fast as the other. That's not to mention the issues of overlay or variable fruit size.
* Violet doesn't mention jar inoculation with hole punch, but I think that would be ideal so they all have uniform inoculum. **X is anything with difficult phenotypes: natalensis or penis envy.
What you see are not phenotypes. It's environmental. Just different fruiting conditions. (ie : different spawn rate/nutrients available, humidity, temperature, FAE,etc...) the exact same, culture will behave differently under different circumstance.
As I wrote above, I did a comparison, I have seen the results. Same conditions, vastly different times to pin. Two tubs of each strain. If you're insisting my conditions were the cause, I'd prefer you not rebut my data with mere beliefs.
|
Lostkeys
Fractal Thought Pattern



Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 months, 23 days
|
|
Here's a very healthy looking culture from an invitro plate pin.
|
gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 6 months, 4 days
|
Re: Psilocybe natalensis [Re: Lostkeys]
#27417669 - 08/07/21 12:11 AM (2 years, 6 months ago) |
|
|
nosf3r4tu, I owe you an apology for my superior/indignant tone above. I checked the tubs and the fast pinning strain was spawned with 1:2 coir, and the slow pinning strain was 1:3 coir. The conditions were indeed different, so we were arguing belief vs belief; I did not have strong data to back my claim. However I still think there's a phenotype difference in speed, since the fast strain also colonized jars faster (identical conditions for sure). The slow strain took its sweet time to pin even after full colonization, while the fast strain vigorously pinned 11 days after spawning, which I consider unusually fast. (Although I don't know all that much about Natalensis, I don't recall people in this thread saying it's a fast pinner--rather the opposite, people have sometimes have trouble getting it to pin.)
|
nosf3r4tu


Registered: 03/26/19
Posts: 775
|
|
Quote:
gone-pear-shaped said:
Quote:
nosf3r4tu said:
Quote:
gone-pear-shaped said: perhaps her strategies would be useful for this species, since its phenotypes are so variable. I only grew out two strains, but one was is twice as fast as the other. That's not to mention the issues of overlay or variable fruit size.
* Violet doesn't mention jar inoculation with hole punch, but I think that would be ideal so they all have uniform inoculum. **X is anything with difficult phenotypes: natalensis or penis envy.
What you see are not phenotypes. It's environmental. Just different fruiting conditions. (ie : different spawn rate/nutrients available, humidity, temperature, FAE,etc...) the exact same, culture will behave differently under different circumstance.
As I wrote above, I did a comparison, I have seen the results. Same conditions, vastly different times to pin. Two tubs of each strain. If you're insisting my conditions were the cause, I'd prefer you not rebut my data with mere beliefs.
In this case it's, strain related(genetics) . But this goes for all species. It's not something that only happens with Natalensis. I was talking about the size and shape of the fruits.
|
gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 6 months, 4 days
|
Re: Psilocybe natalensis [Re: nosf3r4tu]
#27417731 - 08/07/21 02:25 AM (2 years, 6 months ago) |
|
|
I get what you're saying now, thanks. The same strain can be big or small, thin or fat, depending on conditions. Strictly speaking it shouldn't be called a phenotype. But propensity to be a different shape is a phenotype, and in the context I was originally describing (comparing strains in similar grow conditions), I think differing traits should be considered phenotypes, though the wording is a little sloppy.
|
nosf3r4tu


Registered: 03/26/19
Posts: 775
|
|
First flush with a full canopy
 Second flush with 80% less fruits.
|
Eugene Gesuale
Jar-Sniffer



Registered: 04/12/20
Posts: 1,920
Loc: The Basement
Last seen: 10 months, 1 day
|
|
Quote:
alaskappalachian said:
 First 56qt of Adrenognome's potential nat x playa cross starting to pin finally. The tub in his sig from which these genetics originated (you da man btw AG) was sparse and uber snakey, so no shocker. I think this generation will be very similar. Forgot about it and choked it a bit but I want snakes anyway. 
Lookin good Alaska! Any updates? They took me about a week to finish fruiting. The nice thing about snakes is light sporeload, and less waste. They pulled real easy from the sub and dry in 5 hours. A canopy of these would be incredible I notice you also have the tub dialed in pretty good, mine had a lot of rhizomorphic filaments climbing the walls
-------------------- Everything in life is a trade-off. All posts made by this account are purely satirical in nature.
Edited by Eugene Gesuale (08/07/21 10:17 AM)
|
pesa



Registered: 06/13/20
Posts: 604
Last seen: 2 hours, 14 minutes
|
|
plot thickens with Psilocybe natalensis
these shrooms have anti inflammatory, anti oxidants, anti HIV, anti malaria, anti bacterial, anti microbial and possibility of revealing secrets of universe and potential to cure chronic inflammation and are only magic mushrooms tested and proven to contain all these properties.


and these labs are so sterile mf just skip grain prep and went to substrate, i shit you not "they put spore to sterile substrate"

and according to this report nss and Psilocybe natalensis are same mushroom

they made three kind of extract cold, hot(mushy tea) and ethanol to show results
for full lab results check out the pdf https://www.researchgate.net/figure/Compounds-identified-in-the-cold-water-hot-water-and-70-ethanol-extracts-of-P_tbl1_343999450
pic tax hunting for clone, waiting for ice cream to do its thing on cow manure/verm 50/50
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 2 hours, 4 minutes
|
Re: Psilocybe natalensis [Re: pesa] 1
#27418704 - 08/07/21 06:21 PM (2 years, 6 months ago) |
|
|
Spore to substrate could just mean pf cakes.
--------------------
LAGM2020     
|
pesa



Registered: 06/13/20
Posts: 604
Last seen: 2 hours, 14 minutes
|
Re: Psilocybe natalensis [Re: A.k.a]
#27418734 - 08/07/21 06:58 PM (2 years, 6 months ago) |
|
|
Quote:
A.k.a said: Spore to substrate could just mean pf cakes.
it could be, i am contacting lab to get more info on their grow method, have no idea why they miss out on agar spore directly to pf cakes is always a disaster recipe or they are getting super clean spore some how?
Edited by pesa (08/07/21 07:01 PM)
|
Eugene Gesuale
Jar-Sniffer



Registered: 04/12/20
Posts: 1,920
Loc: The Basement
Last seen: 10 months, 1 day
|
Re: Psilocybe natalensis [Re: pesa]
#27418736 - 08/07/21 07:04 PM (2 years, 6 months ago) |
|
|
Dude that is spectacular data! Thank you
-------------------- Everything in life is a trade-off. All posts made by this account are purely satirical in nature.
|
pesa



Registered: 06/13/20
Posts: 604
Last seen: 2 hours, 14 minutes
|
Re: Psilocybe natalensis [Re: pesa]
#27418742 - 08/07/21 07:12 PM (2 years, 6 months ago) |
|
|
thanx and shout out to Crackatoa giveaway  and introducing me to ice cream mycelium
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 2 hours, 4 minutes
|
Re: Psilocybe natalensis [Re: pesa] 1
#27418766 - 08/07/21 07:29 PM (2 years, 6 months ago) |
|
|
It’s possible to use a centrifuge to get clean spores, but also not totally necessary.
--------------------
LAGM2020     
|
|