Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Mono Tub Substrate   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Topicals

Jump to first unread post Pages: < First | < Back | 129 | 130 | 131 | 132 | 133 | 134 | 135 | 136 | 137 | 138 | 139 | 140 | 141 | 142 | 143 | 144 | 145 | 146 | 147 | 148 | 149 | Next > | Last >
InvisibleThe Fresh PrintsS
Smell ya later
Male User Gallery


Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air Flag
Trusted Cultivator
Re: Psilocybe natalensis [Re: mycosavant] * 1
    #27415874 - 08/05/21 06:49 PM (2 years, 6 months ago)

Quote:

mycosavant said:

Very nice! I definitely concur with A.k.a. on that being a solid culture. What's it look like on agar?







This is the culture I used. T1 from Crack's print


--------------------


Extras: Filter Print Post Top
OfflineCrackatoa
Stranger in a strange land
I'm a teapot User Gallery


Registered: 03/31/19
Posts: 5,401
Loc: Over by your Mama's house Flag
Last seen: 1 day, 2 hours
Trusted Cultivator
Re: Psilocybe natalensis [Re: The Fresh Prints]
    #27415956 - 08/05/21 07:51 PM (2 years, 6 months ago)

:hotdamn:


--------------------

Crack's Tek's and Shit



Extras: Filter Print Post Top
OfflineLostkeys
Fractal Thought Pattern
Male User Gallery


Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 months, 23 days
Re: Psilocybe natalensis [Re: The Fresh Prints]
    #27416102 - 08/05/21 09:20 PM (2 years, 6 months ago)

Quote:

The Fresh Prints said:
Quote:

mycosavant said:

Very nice! I definitely concur with A.k.a. on that being a solid culture. What's it look like on agar?







This is the culture I used. T1 from Crack's print




I have a few plates that look like that. Got the spores from a guy on reddit.


Extras: Filter Print Post Top
Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 6 months, 4 days
Re: Psilocybe natalensis [Re: Lostkeys]
    #27416236 - 08/06/21 12:34 AM (2 years, 6 months ago)

I've been reading some of Violet's teks recently, and perhaps her strategies would be useful for this species, since its phenotypes are so variable. I only grew out two strains, but one was is twice as fast as the other. That's not to mention the issues of overlay or variable fruit size.

Violet uses petri dishes very briefly, only to get clean seeming growth*, then grows on grass seed (or brown rice) to rank strains based on colonization speed, pinning characteristics, and other traits with a faster turnaround than with normal methods. I've always wanted to grow ten strains of X** and compare them, but I can't manage that many jars and tubs. I end up with overgrown neglected petri dishes.
https://www.shroomery.org/forums/showflat.php/Number/24856009

* Violet doesn't mention jar inoculation with hole punch, but I think that would be ideal so they all have uniform inoculum.
**X is anything with difficult phenotypes: natalensis or penis envy.


Extras: Filter Print Post Top
OfflineLostkeys
Fractal Thought Pattern
Male User Gallery


Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 months, 23 days
Re: Psilocybe natalensis [Re: gone-pear-shaped]
    #27416472 - 08/06/21 07:54 AM (2 years, 6 months ago)

Quote:

gone-pear-shaped said:
I've been reading some of Violet's teks recently, and perhaps her strategies would be useful for this species, since its phenotypes are so variable. I only grew out two strains, but one was is twice as fast as the other. That's not to mention the issues of overlay or variable fruit size.

Violet uses petri dishes very briefly, only to get clean seeming growth*, then grows on grass seed (or brown rice) to rank strains based on colonization speed, pinning characteristics, and other traits with a faster turnaround than with normal methods. I've always wanted to grow ten strains of X** and compare them, but I can't manage that many jars and tubs. I end up with overgrown neglected petri dishes.
https://www.shroomery.org/forums/showflat.php/Number/24856009

* Violet doesn't mention jar inoculation with hole punch, but I think that would be ideal so they all have uniform inoculum.
**X is anything with difficult phenotypes: natalensis or penis envy.




I sort of used a variation of her in vitro method, except using brf mix per bodhisatta. It isn't colonizing very fast though.


--------------------
So heavy I fell through the earth...



Extras: Filter Print Post Top
Invisiblenosf3r4tu


Registered: 03/26/19
Posts: 775
Re: Psilocybe natalensis [Re: gone-pear-shaped]
    #27416611 - 08/06/21 09:22 AM (2 years, 6 months ago)

Quote:

gone-pear-shaped said:
perhaps her strategies would be useful for this species, since its phenotypes are so variable. I only grew out two strains, but one was is twice as fast as the other. That's not to mention the issues of overlay or variable fruit size.

* Violet doesn't mention jar inoculation with hole punch, but I think that would be ideal so they all have uniform inoculum.
**X is anything with difficult phenotypes: natalensis or penis envy.



What you see are not phenotypes. It's environmental. Just different fruiting conditions. (ie : different spawn rate/nutrients available, humidity, temperature, FAE,etc...) the exact same, culture will behave differently under different circumstance.


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 2 hours, 4 minutes
Trusted Cultivator
Re: Psilocybe natalensis [Re: nosf3r4tu]
    #27416623 - 08/06/21 09:30 AM (2 years, 6 months ago)

I think this is the best, most normal natal tub I’ve ever had :lol:




--------------------
LAGM2020


Extras: Filter Print Post Top
Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 6 months, 4 days
Re: Psilocybe natalensis [Re: nosf3r4tu]
    #27416631 - 08/06/21 09:42 AM (2 years, 6 months ago)

Quote:

nosf3r4tu said:
Quote:

gone-pear-shaped said:
perhaps her strategies would be useful for this species, since its phenotypes are so variable. I only grew out two strains, but one was is twice as fast as the other. That's not to mention the issues of overlay or variable fruit size.

* Violet doesn't mention jar inoculation with hole punch, but I think that would be ideal so they all have uniform inoculum.
**X is anything with difficult phenotypes: natalensis or penis envy.



What you see are not phenotypes. It's environmental. Just different fruiting conditions. (ie : different spawn rate/nutrients available, humidity, temperature, FAE,etc...) the exact same, culture will behave differently under different circumstance.



As I wrote above, I did a comparison, I have seen the results. Same conditions, vastly different times to pin. Two tubs of each strain. If you're insisting my conditions were the cause, I'd prefer you not rebut my data with mere beliefs.


Extras: Filter Print Post Top
OfflineLostkeys
Fractal Thought Pattern
Male User Gallery


Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 months, 23 days
Re: Psilocybe natalensis [Re: gone-pear-shaped] * 1
    #27417007 - 08/06/21 02:51 PM (2 years, 6 months ago)

Here's a very healthy looking culture from an invitro plate pin.


Extras: Filter Print Post Top
Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 6 months, 4 days
Re: Psilocybe natalensis [Re: Lostkeys]
    #27417669 - 08/07/21 12:11 AM (2 years, 6 months ago)

nosf3r4tu, I owe you an apology for my superior/indignant tone above. I checked the tubs and the fast pinning strain was spawned with 1:2 coir, and the slow pinning strain was 1:3 coir. The conditions were indeed different, so we were arguing belief vs belief; I did not have strong data to back my claim. However I still think there's a phenotype difference in speed, since the fast strain also colonized jars faster (identical conditions for sure). The slow strain took its sweet time to pin even after full colonization, while the fast strain vigorously pinned 11 days after spawning, which I consider unusually fast. (Although I don't know all that much about Natalensis, I don't recall people in this thread saying it's a fast pinner--rather the opposite, people have sometimes have trouble getting it to pin.)


Extras: Filter Print Post Top
Invisiblenosf3r4tu


Registered: 03/26/19
Posts: 775
Re: Psilocybe natalensis [Re: gone-pear-shaped]
    #27417712 - 08/07/21 01:34 AM (2 years, 6 months ago)

Quote:

gone-pear-shaped said:
Quote:

nosf3r4tu said:
Quote:

gone-pear-shaped said:
perhaps her strategies would be useful for this species, since its phenotypes are so variable. I only grew out two strains, but one was is twice as fast as the other. That's not to mention the issues of overlay or variable fruit size.

* Violet doesn't mention jar inoculation with hole punch, but I think that would be ideal so they all have uniform inoculum.
**X is anything with difficult phenotypes: natalensis or penis envy.



What you see are not phenotypes. It's environmental. Just different fruiting conditions. (ie : different spawn rate/nutrients available, humidity, temperature, FAE,etc...) the exact same, culture will behave differently under different circumstance.



As I wrote above, I did a comparison, I have seen the results. Same conditions, vastly different times to pin. Two tubs of each strain. If you're insisting my conditions were the cause, I'd prefer you not rebut my data with mere beliefs.



In this case it's, strain related(genetics) . But this goes for all species. It's not something that only happens with Natalensis.
I was talking about the size and shape of the fruits.


Extras: Filter Print Post Top
Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 6 months, 4 days
Re: Psilocybe natalensis [Re: nosf3r4tu]
    #27417731 - 08/07/21 02:25 AM (2 years, 6 months ago)

I get what you're saying now, thanks. The same strain can be big or small, thin or fat, depending on conditions. Strictly speaking it shouldn't be called a phenotype. But propensity to be a different shape is a phenotype, and in the context I was originally describing (comparing strains in similar grow conditions), I think differing traits should be considered phenotypes, though the wording is a little sloppy.


Extras: Filter Print Post Top
Invisiblenosf3r4tu


Registered: 03/26/19
Posts: 775
Re: Psilocybe natalensis [Re: gone-pear-shaped]
    #27417787 - 08/07/21 05:06 AM (2 years, 6 months ago)

First flush with a full canopy

Second flush with 80% less fruits.


Extras: Filter Print Post Top
OfflineEugene Gesuale
Jar-Sniffer
Other


Registered: 04/12/20
Posts: 1,920
Loc: The Basement Flag
Last seen: 10 months, 1 day
Re: Psilocybe natalensis [Re: alaskappalachian]
    #27418032 - 08/07/21 10:10 AM (2 years, 6 months ago)

Quote:

alaskappalachian said:

First 56qt of Adrenognome's potential nat x playa cross starting to pin finally.  The tub in his sig from which these genetics originated (you da man btw AG) was sparse and uber snakey, so no shocker.  I think this generation will be very similar.  Forgot about it and choked it a bit but I want snakes anyway.  :devil:



Lookin good Alaska!
Any updates? They took me about a week to finish fruiting.
The nice thing about snakes is light sporeload, and less waste. They pulled real easy from the sub and dry in 5 hours. A canopy of these would be incredible
I notice you also have the tub dialed in pretty good, mine had a lot of rhizomorphic filaments climbing the walls


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.


Edited by Eugene Gesuale (08/07/21 10:17 AM)


Extras: Filter Print Post Top
Offlinepesa
Male User Gallery


Registered: 06/13/20
Posts: 604
Last seen: 2 hours, 14 minutes
Re: Psilocybe natalensis [Re: Eugene Gesuale] * 1
    #27418674 - 08/07/21 05:54 PM (2 years, 6 months ago)

plot thickens with Psilocybe natalensis

these shrooms have anti inflammatory, anti oxidants, anti HIV, anti malaria, anti bacterial, anti microbial  and possibility of revealing secrets of universe and potential to cure chronic inflammation and are only magic mushrooms tested and proven to contain all these properties.





and these labs are so sterile mf just skip grain prep and went to substrate, i shit you not "they put spore to sterile substrate"




and according to this report nss and Psilocybe natalensis are same mushroom



they made three kind of extract cold, hot(mushy tea) and ethanol to show results

for full lab results check out the pdf
https://www.researchgate.net/figure/Compounds-identified-in-the-cold-water-hot-water-and-70-ethanol-extracts-of-P_tbl1_343999450

pic tax hunting for clone, waiting for ice cream to do its thing on cow manure/verm 50/50



Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 2 hours, 4 minutes
Trusted Cultivator
Re: Psilocybe natalensis [Re: pesa] * 1
    #27418704 - 08/07/21 06:21 PM (2 years, 6 months ago)

Spore to substrate could just mean pf cakes.


--------------------
LAGM2020


Extras: Filter Print Post Top
Offlinepesa
Male User Gallery


Registered: 06/13/20
Posts: 604
Last seen: 2 hours, 14 minutes
Re: Psilocybe natalensis [Re: A.k.a]
    #27418734 - 08/07/21 06:58 PM (2 years, 6 months ago)

Quote:

A.k.a said:
Spore to substrate could just mean pf cakes.




it could be, i am contacting lab to get more info on their grow method, have no idea why they miss out on agar spore directly to pf cakes is always a disaster recipe or they are getting super clean spore some how?


Edited by pesa (08/07/21 07:01 PM)


Extras: Filter Print Post Top
OfflineEugene Gesuale
Jar-Sniffer
Other


Registered: 04/12/20
Posts: 1,920
Loc: The Basement Flag
Last seen: 10 months, 1 day
Re: Psilocybe natalensis [Re: pesa]
    #27418736 - 08/07/21 07:04 PM (2 years, 6 months ago)

Dude that is spectacular data! Thank you


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.


Extras: Filter Print Post Top
Offlinepesa
Male User Gallery


Registered: 06/13/20
Posts: 604
Last seen: 2 hours, 14 minutes
Re: Psilocybe natalensis [Re: pesa]
    #27418742 - 08/07/21 07:12 PM (2 years, 6 months ago)

thanx and shout out to Crackatoa giveaway :snoopyes:
and introducing me to ice cream mycelium:laugh2:


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 2 hours, 4 minutes
Trusted Cultivator
Re: Psilocybe natalensis [Re: pesa] * 1
    #27418766 - 08/07/21 07:29 PM (2 years, 6 months ago)

It’s possible to use a centrifuge to get clean spores, but also not totally necessary.


--------------------
LAGM2020


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 129 | 130 | 131 | 132 | 133 | 134 | 135 | 136 | 137 | 138 | 139 | 140 | 141 | 142 | 143 | 144 | 145 | 146 | 147 | 148 | 149 | Next > | Last >

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Mono Tub Substrate   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Topicals


Similar ThreadsPosterViewsRepliesLast post
* Help a girl out with making grain spawn for the first time - P. natalensis slow growth and light blue color on 1 jar (contam?) psilocybe-maria 254 9 02/15/24 04:06 PM
by psilocybe-maria
* Official Psilocybe zapotecorum thread
( 1 2 3 4 ... 25 26 )
Hindsight 17,912 513 02/16/24 05:17 PM
by YoshiTrainer
* Psilocybe Menace!
( 1 2 3 4 5 all )
P.Menace 9,899 88 06/07/08 06:01 AM
by wisp
* Strange bulbous mushroom alongside Psilocybe cubensis Shroomdee 218 11 02/12/24 07:22 AM
by HazMatt
* Psilocybe Mexicana Strain-A - Sclerotia ???
( 1 2 all )
76degrees 13,596 26 06/19/06 11:27 AM
by coda
* psilocybe cyanescens
( 1 2 all )
mikeownow 5,609 29 12/03/16 04:08 PM
by amidogen
* Psilocybe weilii indoors Chief_ 3,869 7 01/14/12 12:09 AM
by dtowntoker

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
333,237 topic views. 41 members, 154 guests and 61 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.178 seconds spending 0.115 seconds on 26 queries and 0.033 seconds on Sphinx.