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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: PrimalSoup]
#27411373 - 08/01/21 11:19 PM (2 years, 6 months ago) |
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Try running out of water while steam pasteurizing a turkey bag full of shredded fire cured tobacco. That'll make you smell like fried butthole for a week.
Well pouring lc turned out to be a fairly clean procedure. Two of mine lc-pour jars caught the creeping crud but everyone else is still banging ass. Melmak lcs colonizing nicely. Going into town 2mrrw for 10bux worth of that sweet wheat goodness. Shud have the entirety of my jars at some form of colonization in a few days. Will be nice to spawn some clean shit again. Lotta Ape too
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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 6 months, 18 days
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Re: Cultivation General Discussion [Re: Feasoghorm]
#27411383 - 08/01/21 11:42 PM (2 years, 6 months ago) |
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I've also been thinking LC is well suited to PE due to possible slower colonization. Anybody know if PE LC can be refrigerated? I'm paranoid about opening LC, so I was thinking of cooling it down, then testing it on agar, and then using it all if the test was satisfactory. So if it contams when opened, the contam load will still be small when it's used a few days later. (Jar is filtered, also mostly full, so temp changes sucking air in and out won't be a huge problem.) This is what I do with food sometimes: don't open it until it's cold, so there are zero minutes of time at room temperature after opening.
I know it's a bit paranoid, but will refrigeration do more harm than good?
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Salvitica
Stranger


Registered: 04/27/21
Posts: 45
Last seen: 2 years, 5 months
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I have exploded another bag today. Yay me! 
One day, they wont explode. I am tired of cleaning it up/
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 9 months, 7 days
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Re: Cultivation General Discussion [Re: Salvitica]
#27411424 - 08/02/21 01:01 AM (2 years, 6 months ago) |
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wahhwahh
stupid idiot


Registered: 07/19/21
Posts: 296
Last seen: 1 year, 10 months
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Re: Cultivation General Discussion [Re: BrownBear]
#27411436 - 08/02/21 01:17 AM (2 years, 6 months ago) |
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Aight so I order spores 5 months ago before I knew wtf I was doing. I've grow Z and PE#6 so far. After a MEAGER second flush they're both farting out. One with trich it seems, other just growing small shrooms randomly.
Neither grew very big fruit, but the vendor seems clean.
What should I do for next two? I have AA+ and "Amazonian" that seem intriguing. Others are "blue meanies" (ended up cube variety bleh) and "jedi mind fuck" which just seems to be a generic cube.
I have 24 petri PDA plates. I also have a fuckton of grain. Would it be quicker to just MSS to grain, or do agar from MSS? I plan to b2s the first two quarts that fully colonize either way.
I got the agar to clone from my first batch but all the fruit was meh-size so didn't bother.
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 6 days, 11 hours
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Re: Cultivation General Discussion [Re: wahhwahh]
#27411451 - 08/02/21 01:40 AM (2 years, 6 months ago) |
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Clean up a culture before putting it on grain.
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: Failboat]
#27411508 - 08/02/21 04:14 AM (2 years, 6 months ago) |
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I havent found refrigeration of lc to harm it, but your process seems unnecessary.
An lc is opened twice in its life: When it's inoculated, and when you pour it into many jars of succulent winter white. Anything else is unnecessary and is an opportunity for contamination to enter the clandestine god jizz that is lc. Trust in your sterile procedure.
I cant wait to try with a little agar in it. Mycelium sludge. Less liquid in the grain.
Brownbear! I was having withdrawals god damnit where were ya?. Dnt ever leave me again
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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 6 months, 18 days
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Re: Cultivation General Discussion [Re: Feasoghorm]
#27411518 - 08/02/21 05:09 AM (2 years, 6 months ago) |
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Quote:
Feasoghorm said: I cant wait to try with a little agar in it. Mycelium sludge. Less liquid in the grain.
Wow, we really have been thinking alike. My current attempt is semi-solid LC, with 0.2% each of agar and LME. I think more agar would be okay. The tek author didn't observe the jar being too wet as a result of a large LC inoculation.
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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 9,654
Loc: South Africa
Last seen: 24 seconds
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Stick to 0,3 - 0,5g agar, I added dextrose and lme at a very low ratio as well, mycelium just grows so well this way due to being suspended.
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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 6 months, 18 days
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Re: Cultivation General Discussion [Re: DERRAYLD]
#27411557 - 08/02/21 06:37 AM (2 years, 6 months ago) |
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Quote:
DERRAYLD said: Stick to 0,3 - 0,5g agar, I added dextrose and lme at a very low ratio as well, mycelium just grows so well this way due to being suspended.
Per 200 mL water, right?
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,825
Loc: Gaming the system
Last seen: 4 hours, 50 minutes
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Syringe to grain would be quicker if it works, sometimes I’ll put spores to grain and plates at the same time. That way you might pull off a decent tub or at least enough to get a quick clone.
--------------------
LAGM2020     
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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 9,654
Loc: South Africa
Last seen: 24 seconds
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Quote:
gone-pear-shaped said:
Quote:
DERRAYLD said: Stick to 0,3 - 0,5g agar, I added dextrose and lme at a very low ratio as well, mycelium just grows so well this way due to being suspended.
Per 200 mL water, right?
Yeh that's it, I started with 0.5g agar, 1g lme and 1g dextrose but it was a bit firm for me after cooling so I diluted it further to around 0.3g agar for that liquid gel consistency.
Mycelium grows everywhere though so be ready.

You can see the mycelium growing on the jar walls.
Edited by DERRAYLD (08/02/21 06:59 AM)
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,244
Last seen: 6 minutes, 13 seconds
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Re: Cultivation General Discussion [Re: DERRAYLD]
#27411577 - 08/02/21 07:10 AM (2 years, 6 months ago) |
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Awesome stuff I just made up 5 broths last night 3 are 250ml/.5LME/(2 got .5 agar) And 2 smaller 150ml. 1 with agar and 1 w/o
Made sure to sterilize a 10ml syringe with them. Getting my poke on tonight
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,819
Loc: Canada
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Re: Cultivation General Discussion [Re: DERRAYLD]
#27411582 - 08/02/21 07:20 AM (2 years, 6 months ago) |
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Quote:
DERRAYLD said:
Quote:
gone-pear-shaped said:
Quote:
DERRAYLD said: Stick to 0,3 - 0,5g agar, I added dextrose and lme at a very low ratio as well, mycelium just grows so well this way due to being suspended.
Per 200 mL water, right?
Yeh that's it, I started with 0.5g agar, 1g lme and 1g dextrose but it was a bit firm for me after cooling so I diluted it further to around 0.3g agar for that liquid gel consistency.
Mycelium grows everywhere though so be ready.

You can see the mycelium growing on the jar walls.
Looks turbid. Mycelium growing on the walls kind of adds to that. I would test those before using.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,940
Loc: ation
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Re: Cultivation General Discussion [Re: Pastywhyte] 1
#27411604 - 08/02/21 07:54 AM (2 years, 6 months ago) |
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Sslc always looks a bit turbid cause the added agar adds a bit of opaqueness.
The tek calls for .2% agar. Any more and you get solidification. In the tek I made 250ml bottles so .2% is half a gram of agar.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,819
Loc: Canada
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Re: Cultivation General Discussion [Re: natedawgnow]
#27411617 - 08/02/21 08:05 AM (2 years, 6 months ago) |
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Hmm that’s interesting to know. Personally I would have taken if for granted as bacteria with that much opacity. I may need to try some SSLC next time just for the experience.
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E_Dubbya


Registered: 05/14/21
Posts: 609
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Re: Cultivation General Discussion [Re: Pastywhyte] 1
#27411632 - 08/02/21 08:23 AM (2 years, 6 months ago) |
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I made some the other day and did a whoopsie with too much agar. Not fully solid so I figured why not give em a go still. Looks like the mycelium likes it so far. It's about the consistency of hair gel.
-------------------- Trade List
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monkey_accident
fail now learn later


Registered: 05/10/19
Posts: 678
Loc: im here, bro
Last seen: 5 months, 24 days
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Re: Cultivation General Discussion [Re: E_Dubbya]
#27411688 - 08/02/21 09:33 AM (2 years, 6 months ago) |
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might just go ahead and order new gaskets and guages for my prestos before something goes wrong and I waste materials been using them for awhile with no repair other than some lubing so why not give them some tlc
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wahhwahh
stupid idiot


Registered: 07/19/21
Posts: 296
Last seen: 1 year, 10 months
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Re: Cultivation General Discussion [Re: A.k.a]
#27411922 - 08/02/21 01:43 PM (2 years, 6 months ago) |
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Quote:
A.k.a said: Syringe to grain would be quicker if it works, sometimes I’ll put spores to grain and plates at the same time. That way you might pull off a decent tub or at least enough to get a quick clone.
This is what I was assuming. I'm going to do both today. Like I said, I have a TON of grain, I'm not worried about some of them not working out, I'll live. I just want another tub going ASAP.
Edited by wahhwahh (08/02/21 01:44 PM)
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0ptiquest
Stranger


Registered: 07/05/20
Posts: 509
Last seen: 2 days, 21 hours
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Re: Cultivation General Discussion [Re: BrownBear]
#27411932 - 08/02/21 01:58 PM (2 years, 6 months ago) |
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Quote:
Iambrownbear said: Try my tek https://www.shroomery.org/forums/showflat.php/Number/27338273/page/2 if you haven't already.
Have been using this tek for bags, so simple and works great for grain and substrate.
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