Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals Bridgetown Botanicals   Kraken Kratom Kratom Capsules for Sale   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Substrate Bags   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Autoflowering Cannabis Seeds   OlympusMyco.com Olympus Myco Bulk Substrate   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5  [ show all ]
OfflineTheMagicConch
MC
Male User Gallery


Registered: 09/11/20
Posts: 841
Loc: Over here
Last seen: 6 months, 7 days
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: multifractal]
    #27430813 - 08/16/21 07:54 PM (2 years, 8 months ago)

My apologies of this doesn't technically have to do with the life cycle, but I thought this may be relevant since this is something we see all the time in cultivation.

I just read in "The Growing Fungus":
"In higher fungi, the spread of damage from one compartment to others can be prevented by the prompt blocking of septal pores. This can occur either through the occlusion of pores by protoplasmic inclusions such as crystals or by the rapid synthesis of pore plugs."

Do any of you think this is why blueing occurs on active species? If I'm understanding this right, the actives are using the psilocin crystal as a protoplasmic inclusion as a response to damage (touching the mushroom or mycelium with your fingers etc) and since that's being exposed to oxygen (being on the outer surface) it oxidizes and turns blue?

Not sure if that's accurate, but thought it was intriguing

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,843
Loc: Canada Flag
Trusted Cultivator
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: TheMagicConch]
    #27430827 - 08/16/21 08:00 PM (2 years, 8 months ago)

This question is definitely on point in this thread, I'll wait for others to answer this however. I don't know enough to respond further.

Extras: Filter Print Post Top
OfflineTheMagicConch
MC
Male User Gallery


Registered: 09/11/20
Posts: 841
Loc: Over here
Last seen: 6 months, 7 days
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: Stipe-n Cap] * 1
    #27430835 - 08/16/21 08:04 PM (2 years, 8 months ago)

Good to hear. Didn't want to take this in a different direction than you intended:thumbup:

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,843
Loc: Canada Flag
Trusted Cultivator
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: TheMagicConch]
    #27430839 - 08/16/21 08:07 PM (2 years, 8 months ago)

That is a great book, definitely worth reading. Bod definitely hooked us up with that link, it's quite expensive to purchase.

Extras: Filter Print Post Top
OfflineTheMagicConch
MC
Male User Gallery


Registered: 09/11/20
Posts: 841
Loc: Over here
Last seen: 6 months, 7 days
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: Stipe-n Cap]
    #27430879 - 08/16/21 08:51 PM (2 years, 8 months ago)

Yeah for sure. I saw him mention it and actually found it on another website I use for books, in case anyone reading this thread is interested here's a link to it https://1lib.us/

They have other great mycology titles on that website as well. The link from bod looks like a very similar website so it'd probably have the same titles anyways, but just in case.

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,843
Loc: Canada Flag
Trusted Cultivator
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: TheMagicConch]
    #27529224 - 11/03/21 07:17 PM (2 years, 6 months ago)

Post here for life cycle debate, let's keep the potd thread clear, I recommen reading from the beginning and understanding the principles and terminology before posting.

Extras: Filter Print Post Top
Invisiblemultifractal
Student
 User Gallery

Registered: 05/08/15
Posts: 1,019
Loc: Cumtown
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: TheMagicConch]
    #27530719 - 11/04/21 11:59 PM (2 years, 6 months ago)

Quote:

TheMagicConch said:
My apologies of this doesn't technically have to do with the life cycle, but I thought this may be relevant since this is something we see all the time in cultivation.

I just read in "The Growing Fungus":
"In higher fungi, the spread of damage from one compartment to others can be prevented by the prompt blocking of septal pores. This can occur either through the occlusion of pores by protoplasmic inclusions such as crystals or by the rapid synthesis of pore plugs."

Do any of you think this is why blueing occurs on active species? If I'm understanding this right, the actives are using the psilocin crystal as a protoplasmic inclusion as a response to damage (touching the mushroom or mycelium with your fingers etc) and since that's being exposed to oxygen (being on the outer surface) it oxidizes and turns blue?

Not sure if that's accurate, but thought it was intriguing



Psilocin is in solution in the intracellular fluid and not found in significant  quantities as a crystal in living tissue. Bruising occurs as cells rupture exposing psilocin to oxygen causing it to oxidize to a blue/green pigment. Its hypothesized that psilocin production might have evolved as a mechanism to store house nitrogen/other building blocks and might also have proved useful as a pest deterrent of sorts but I've never read that its breakdown specifically serves any purpose ie cell damage reduction or something.

Edited by multifractal (11/05/21 12:28 AM)

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,843
Loc: Canada
Trusted Cultivator
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: multifractal]
    #27531090 - 11/05/21 09:20 AM (2 years, 6 months ago)

I don't think that this mentioned bruising but very interesting nonetheless:

Horizontal gene cluster transfer increased hallucinogenic mushroom diversity

https://onlinelibrary.wiley.com/doi/10.1002/evl3.42

Extras: Filter Print Post Top
OfflineBrock one
Stranger
Male User Gallery

Registered: 11/24/21
Posts: 9
Loc: Uk Flag
Last seen: 6 months, 11 days
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: Stipe-n Cap]
    #27557023 - 11/25/21 01:21 AM (2 years, 5 months ago)

So breeding mushrooms isn't to dislike breeding plants? I don't get how breeding a pheno work's in mushroom breeding. I kinda get it but dont understand spores versus pollen just yet. Pheno is just a single individual set of genes from a genome in plants. But thats why I am here to learn these things so I better get some reading and researching done I guess  :takingnotes:  Nice thread by the way Full Retarded. I have only breed plants in the past. Tissue culture ect. Got a bit of a reputation for breeding cannabis on online forums at a point. I Come here to learn more about growing shrooms to treat my mental health but its a interesting thing this mushroom growing. :thumbup:

Extras: Filter Print Post Top
InvisibleJustAPerson
Stranger
 User Gallery

Registered: 01/23/21
Posts: 22
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: multifractal]
    #27557322 - 11/25/21 08:35 AM (2 years, 5 months ago)

Quote:

multifractal said:
Quote:

TheMagicConch said:
My apologies of this doesn't technically have to do with the life cycle, but I thought this may be relevant since this is something we see all the time in cultivation.

I just read in "The Growing Fungus":
"In higher fungi, the spread of damage from one compartment to others can be prevented by the prompt blocking of septal pores. This can occur either through the occlusion of pores by protoplasmic inclusions such as crystals or by the rapid synthesis of pore plugs."

Do any of you think this is why blueing occurs on active species? If I'm understanding this right, the actives are using the psilocin crystal as a protoplasmic inclusion as a response to damage (touching the mushroom or mycelium with your fingers etc) and since that's being exposed to oxygen (being on the outer surface) it oxidizes and turns blue?

Not sure if that's accurate, but thought it was intriguing



Psilocin is in solution in the intracellular fluid and not found in significant  quantities as a crystal in living tissue. Bruising occurs as cells rupture exposing psilocin to oxygen causing it to oxidize to a blue/green pigment. Its hypothesized that psilocin production might have evolved as a mechanism to store house nitrogen/other building blocks and might also have proved useful as a pest deterrent of sorts but I've never read that its breakdown specifically serves any purpose ie cell damage reduction or something.




Yup. Here is the chemistry of the blueing.
Science

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,843
Loc: Canada
Trusted Cultivator
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: Brock one]
    #27557459 - 11/25/21 10:18 AM (2 years, 5 months ago)

Quote:

Brock one said:
So breeding mushrooms isn't to dislike breeding plants?



Breeding/stabilization is somewhat simple:

Select for desired trait(s)(Clone)/grow out clone, collect spores from that grow then grow out next generation; clone again then grow out, collect spores and so on until you have what youre looking for.

That's the basic idea.

Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,843
Loc: Canada
Trusted Cultivator
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: Stipe-n Cap]
    #28125398 - 01/05/23 07:54 AM (1 year, 4 months ago)

Quote:

p9hu7 said:
I've actually just sent of an email to one of the PhD mycology professors there, hopefully I'll receive a reply.

My message reads:

My question(s) to you, if you're willing to answer, are:

Do  Basidiamycota form hyphal knots from rhizomorphs symphogenously, or are they meristogenous.




I never did receive a response from that mycologist, but an AI chat bot had no issues answering this question.

There were a few more sent to that mycologist which I wall ask the chat bot after morning coffee.


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,843
Loc: Canada
Trusted Cultivator
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: Stipe-n Cap]
    #28125467 - 01/05/23 09:01 AM (1 year, 4 months ago)

Quote:

p9hu7 said:
2 haploid basidiospore germinate to form monokaryotic hyphae, if compatable they fuse by anastomosis,  plasmogamy fuses protoplasts and become binucleate/dikaryotic, dikaryotic hypha fuse via clamp connection to other monokaryotic hypha forming rhizomorphs, rhizomorphs produce basidiocarp containing many individual dikaryotic strains.

Is this correct?

We will see what he has to say on the matter, if anything.






Great answers. AI is such a great resource  for Q&A.

Jesus, this thing is awesome:

Quote:

p9hu7 said:
Just had a thought:

Basidaia are formed at the tip of dikaryotic hyphae, each basidia forms 4 spores. If you could get a decent estimate of how many spores were contained in a print you could divide that number by four and you'd have a rough estimate of how many dikaryotic hyphae made up that fruit.

I would suspect that other features of the  basidiocarp that are also made from compact mycelium, like the gills, must be mycelium that either hasn't successfully mated (successfully mating is required before hyphae can fruit), or do not carry the genes to form basidia, but perhaps the hyphae form all of these features with basidia simply manifesting at the tip.

Either way it's about as close to an estimate as you're likely to achieve, with that number being the minimum presence of dikaryotic hyphae.

Does anyone see any issues with this reasoning?






Edited by Stipe-n Cap (01/05/23 09:25 AM)

Extras: Filter Print Post Top
Invisiblefungusul
Fungus Kingdom


Registered: 07/16/20
Posts: 1,065
Re: Basidiomycetes life cycle discussions: karyogamy, meiosis, anastomosis, etc [Re: Stipe-n Cap]
    #28126346 - 01/05/23 06:31 PM (1 year, 4 months ago)

:takingnotes:

Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5  [ show all ]

Shop: Bridgetown Botanicals Bridgetown Botanicals   Kraken Kratom Kratom Capsules for Sale   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Substrate Bags   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Autoflowering Cannabis Seeds   OlympusMyco.com Olympus Myco Bulk Substrate   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder


Similar ThreadsPosterViewsRepliesLast post
* Required dark cycle
( 1 2 all )
spliffmasta 3,727 28 12/14/04 04:42 PM
by EonTan
* G2G transfers...an endless cycle? ADoobie 7,304 18 11/12/20 07:10 PM
by FredsGotSlacks
* Contest!!! Be an Agar Star
( 1 2 3 4 all )
egghead 8,552 68 05/27/01 03:33 PM
by m0ck5
* Agar Contest Winners egghead 1,823 13 05/29/01 06:55 AM
by Datrutankdogg69
* test tubes agar fungophiliac 3,193 8 06/19/20 09:41 PM
by gorilliam
* Tacky Daddies 3 Layer Merv Flow Hood. Clean Agars. BiggDaddyCane 470 18 01/01/23 11:28 AM
by SwabMarley
* BIO?: Mitosis/Meiosis when does one end and the other begin in fungi? aliesterlavey 657 12 01/13/10 09:28 PM
by ScavengerType
* The official Psilocybe natalensis thread
( 1 2 3 4 ... 549 550 )
Hillbillyjohn 359,229 10,985 05/17/24 09:43 PM
by fahtster

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,463 topic views. 12 members, 80 guests and 47 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.005 seconds on 12 queries.