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OfflineRoyale

Registered: 03/24/04
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WBS Contam?? (PICS)
    #2738413 - 05/27/04 09:40 PM (12 years, 6 months ago)

O.k. I am sorry if this has been covered somewhere and I was unable to find it. But I will have lost 6 quart jars to this and it is starting to bother me. I am using Pennington's WBS from Wal-Mart. I soak for 24hours, then simmer for 30min. Then strain and rinse. Then load into quart jars, then cover lid with coffee filter and place in PC. Jars are PC'ed for 1hour to 1hour 15min. Let cool, and knock up. 4 of the 6 jars lost were a BRF to WBS transfer. But these last 2 jars were from a syringe I made from a print I made. So, I am thinking it has something to do with the sterilization of my WBS. After a few days in the incubator I notice yellowish/brownish/glodish dust in certain areas of my jars. I spreads fairly rapid, but not really really fast. I had thought that my jars were drying out. In an attempt to salvage the first 4 jars I lost I removed contents into a casing tray and sprayed with water/h2o2 solution. I noticed a sweet smell comming from the WBS. I ended up tossing this out because after 3 or 4 days it still looked dryed out. Now it's back and I am at a loss.
Any ideas on what this is? Thanks you!!!



--------------------
"Burnin', I feel a burnin' in my stomach
I wanna' know if I'm a gonna make it
If I don't just spread my ashes
If I do just spread your mind"
~~~~~~PRIMUS~~~~~~


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OfflineSuntzu
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Re: WBS Contam?? (PICS) [Re: Royale]
    #2738610 - 05/27/04 10:25 PM (12 years, 6 months ago)

It sounds like bacteria and mold. Some recommendations:
Try a different brand of WBS. Go as millet-heavy as possible.
Alter the grain prep; Different people can get different results from the same recipe. Regardless of the tek, I think it's a good idea to thoroughly rinse birdseed beforehand.
Cut the PC time down to an hour; IME grains burst more beyond that, freeing up starches that bacteria love. An hour should be fine.
Cool the PC in a clean area, covering vent with soaked rag or such.
Think about double-coffee filter, if not completely overhauling the filter system. The combination of a good filtering system and 'clean cooling' will do a LOT to cut down on contaminants.
Double check your inoculating system. Is the air clean during inoc's?
Good luck, WBS is awesome once you get it down.


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OfflineRoyale

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Re: WBS Contam?? (PICS) [Re: Suntzu]
    #2740556 - 05/28/04 08:15 AM (12 years, 6 months ago)

Thank you for the info. I'm gonna move back down to 1/2 pint jars and try again. I was really looking forward to getting my WBS casing going, but guess I gotta iron out some problems first. I did not rinse as well as I probably should have, I underestimated the importance of this due to the fact that I would be PCing the mix later. Well, wish me luck and thanks again!


--------------------
"Burnin', I feel a burnin' in my stomach
I wanna' know if I'm a gonna make it
If I don't just spread my ashes
If I do just spread your mind"
~~~~~~PRIMUS~~~~~~


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OfflineSuntzu
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Re: WBS Contam?? (PICS) [Re: Royale]
    #2740887 - 05/28/04 11:01 AM (12 years, 6 months ago)

No problem, but I forgot a very important one:
When experimenting or ironing out problems, it's a good idea to keep back a negative control or two: jar(s) that have not been inoculated but are shaken and incubated with the others. One jar could be an unopened negative control, one could be a 'fake-inoculated' negative control. Both may not be necessary, but keeping one negative is a very good idea. If it does not go bad it can be inoculated later. These things are never perfect. If you're only losing 4/6 jars, your negative control may be fine even though jar prep is to blame. But if an uninoculated, unopened jar DOES go bad, that can really tell you something.


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OfflineRoyale

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Re: WBS Contam?? (PICS) [Re: Suntzu]
    #2741525 - 05/28/04 02:49 PM (12 years, 6 months ago)

Very good idea. I will keep that in mind. I honestly have no idea at what stage I am picking up or not getting rid of the contam. I need to invest in some tyvek, and rinse better. Hopefully this will help. Thanks again.


--------------------
"Burnin', I feel a burnin' in my stomach
I wanna' know if I'm a gonna make it
If I don't just spread my ashes
If I do just spread your mind"
~~~~~~PRIMUS~~~~~~


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Wild Bird Seed

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