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Offlinetututotutut
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Re: Recognizing and dealing with contamination [Re: milkboy]
    #27380225 - 07/09/21 10:34 AM (4 months, 17 days ago)

I can't even remember how I did mine. Probably torch and (wrong type of) knife 🤣


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Offlinemonkey_accident
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Re: Recognizing and dealing with contamination [Re: tututotutut]
    #27380638 - 07/09/21 04:14 PM (4 months, 17 days ago)


here's some bacterial wet grain spawn jars I have
i assume the grains were too wet when I loaded them


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OfflineB1rdMan
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Re: Recognizing and dealing with contamination [Re: monkey_accident]
    #27380650 - 07/09/21 04:19 PM (4 months, 17 days ago)

Im still learning but seeing this i would have thought it was still good. Could you point out where you see the bacteria growth? Is it the flecks of black in there?


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OfflineBrownBear
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Re: Recognizing and dealing with contamination [Re: B1rdMan] * 1
    #27380660 - 07/09/21 04:28 PM (4 months, 17 days ago)

The tell tale signs are there are uncolonized grain. Around the perimeter of the uncolonized grain the mycelium is growing thicker but won't colonize the grain.


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Offlinemonkey_accident
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Re: Recognizing and dealing with contamination [Re: BrownBear] * 1
    #27380690 - 07/09/21 04:48 PM (4 months, 17 days ago)

the flecks of black are just pieces of grain/other grain fragments unfavorably pressing on the glass i believe
and as bb said, the signs here are the uncolonized grain and the changes in the growth of the mycelium

you can see where it got to that blue line and started changing, it got thick and brighter white
and kind of "stitched" together in the middle area where it is fighting the bacteria


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OfflineB1rdMan
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Re: Recognizing and dealing with contamination [Re: monkey_accident]
    #27381128 - 07/09/21 10:49 PM (4 months, 17 days ago)

Wow really interesting stuff. Thanks for the breakdown to both of you.


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OfflineSpaceBabyS
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Re: Recognizing and dealing with contamination [Re: monkey_accident] * 1
    #27381197 - 07/10/21 12:05 AM (4 months, 17 days ago)

That could go to quarantined shoeboxes, though, couldn't it? I know a bunch of my jars were bacterial. The concensus was they weren't viable: Since then every box has fruited and I have nearly 14-lbs wet harvested so far, and the fruits keep coming. :shrug: I just have not done more than a 2-quart spawn mini-tub but hey, it's working out. Doing the same with 'susspicious Huatla' and that is colonizing the fastest so far. Mostly cause I dialed in FC better recently.


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Re: Recognizing and dealing with contamination [Re: SpaceBaby] * 1
    #27381210 - 07/10/21 12:20 AM (4 months, 17 days ago)

Yes, that is totally shoebox-able. But for good practice it's good that we just speak of it as garbage as it's of no use otherwise without risk. Don't monotub it, and definitely don't G2G it.


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OfflineSpaceBabyS
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Re: Recognizing and dealing with contamination [Re: Smartattack]
    #27381218 - 07/10/21 12:24 AM (4 months, 17 days ago)

Quote:

Smartattack said:
Yes, that is totally shoebox-able. But for good practice it's good that we just speak of it as garbage as it's of no use otherwise without risk. Don't monotub it, and definitely don't G2G it.




Ah...fruit of the poison tree...:mushroom2:

I'm getting better. The goal is 54-quart monotubs; I'm sure I'll get there.:grin:


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Edited by SpaceBaby (07/10/21 12:25 AM)


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Offlinetututotutut
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Re: Recognizing and dealing with contamination [Re: SpaceBaby]
    #27381279 - 07/10/21 01:30 AM (4 months, 17 days ago)

Bacterial shoeboxes are the source of the majority of my shroom stash right now and im talking it was enough for me to not worry about any xfer/agar/grain/any sab work for weeks lol


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Offlinegone-pear-shaped
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Re: Recognizing and dealing with contamination [Re: B1rdMan]
    #27381334 - 07/10/21 02:32 AM (4 months, 17 days ago)

Quote:

B1rdMan said:
Im still learning but seeing this i would have thought it was still good. Could you point out where you see the bacteria growth? Is it the flecks of black in there?



Have a look here to see a lot of picture examples of good and bad spawn:
https://www.shroomery.org/forums/showflat.php/Number/27187543


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OfflineLittleBoard
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Re: Recognizing and dealing with contamination [Re: gone-pear-shaped]
    #27381410 - 07/10/21 05:47 AM (4 months, 17 days ago)

I would have used that jar without blinking an eye because I would have assumed the mycelium "won" over the bactaeria in the end, but I only do shoeboxes so far.
Really slow jars I would even give another month before throwing them away when they are not fully colonized.
The slow, full colonizers I would spawn separately into ~2 liter (2 quart)ice cream tubs with 50% coir 50% spawn. Almost always with great success.
These "bacterial" tubs often show no contam at all or can do the regular 3 flushes before they contam.

I have another question: I recently had moldy spawn but one shoebox did well and flushed normally. So I cloned from one shroom in there.
Is there mold inside the mushroom, or will the mycelium fight this and not let anything it inside the fruit?
The idea was that I could throw away all the agar plates that lead to the moldy spawn but that I could save the genetic by cloning the survivors.
The plate that I made from them shows good, fast rizomorphic growth as far as I can tell.


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: LittleBoard]
    #27381464 - 07/10/21 08:26 AM (4 months, 17 days ago)

Quote:

LittleBoard said:

I have another question: I recently had moldy spawn but one shoebox did well and flushed normally. So I cloned from one shroom in there.
Is there mold inside the mushroom, or will the mycelium fight this and not let anything it inside the fruit?
The idea was that I could throw away all the agar plates that lead to the moldy spawn but that I could save the genetic by cloning the survivors.
The plate that I made from them shows good, fast rizomorphic growth as far as I can tell.




Since the mold came from the spawn, it's likely enmeshed. Got plate pics?


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Offlinetututotutut
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Re: Recognizing and dealing with contamination [Re: LadysKnight]
    #27381604 - 07/10/21 10:54 AM (4 months, 16 days ago)

Can bacterial agar plates be spawned to coir in a small container or something?


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Offlinegone-pear-shaped
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Re: Recognizing and dealing with contamination [Re: tututotutut]
    #27381606 - 07/10/21 10:55 AM (4 months, 16 days ago)

Yes, it's usually called a shoebox.


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Offlinemonkey_accident
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Re: Recognizing and dealing with contamination [Re: SpaceBaby]
    #27381624 - 07/10/21 11:06 AM (4 months, 16 days ago)

Quote:

SpaceBaby said:
That could go to quarantined shoeboxes, though, couldn't it? I know a bunch of my jars were bacterial. The concensus was they weren't viable: Since then every box has fruited and I have nearly 14-lbs wet harvested so far, and the fruits keep coming. :shrug: I just have not done more than a 2-quart spawn mini-tub but hey, it's working out. Doing the same with 'susspicious Huatla' and that is colonizing the fastest so far. Mostly cause I dialed in FC better recently.




I'm top fruiting that jar, but like smart said its just a waste of time/resources to put more effort into a shitty jar of spawn


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Offlinetututotutut
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Re: Recognizing and dealing with contamination [Re: gone-pear-shaped]
    #27381682 - 07/10/21 11:50 AM (4 months, 16 days ago)

Quote:

gone-pear-shaped said:
Yes, it's usually called a shoebox.



Lol no way really? It'll friggin work?
Ahhhh I've trashed hundreds of potential spawn dishes


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Edited by tututotutut (07/10/21 11:51 AM)


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Offlinegone-pear-shaped
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Re: Recognizing and dealing with contamination [Re: tututotutut]
    #27381716 - 07/10/21 12:32 PM (4 months, 16 days ago)

Quote:

tututotutut said:
Quote:

gone-pear-shaped said:
Yes, it's usually called a shoebox.



Lol no way really? It'll friggin work?
Ahhhh I've trashed hundreds of potential spawn dishes



Oh, sorry! I misread you. I thought you were gonna spawn bacterial grain to coir. That's what shoeboxes (small tubs) are used for.

There is a thing you can do with bacterial plates--maybe more trouble than it's worth unless you need to save that plate. You can set up a 3D environment that mycelia likes but bacteria will have a harder time with, like a test tube full of stuff that cube mycelia like--or a BRF/verm puck, which is more common--then harvest the cube mycelia from the other side.

I think spawning to a jar of BRF/verm like PF Tek would work. But it's different since with PF Tek you inoculate underneath a dry verm layer, but you're gonna inoculate on the surface. I think it will work if you use a jar like a grain jar--sterile with an air filter. And you don't necessarily need to let it grow all the way. You can use the BRF/verm the same way as agar: grow it some, then take some clean growth from the edge. Josex describes it here:
https://www.shroomery.org/forums/showflat.php/Number/24806569/fpart/1/vc/1/nt/8


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OfflineGotfrey
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Re: Recognizing and dealing with contamination [Re: gone-pear-shaped]
    #27381905 - 07/10/21 04:00 PM (4 months, 16 days ago)

Hey yall,

I'm just starting my cultivation journey, and I got some grain spawn that needs assessment by some experienced eyes.

First thing I started was agar. But streaking 20 plates left me with quiet a bit in my syringe. So I knocked up some grain jars.

Shook them at about 15-20%. One of the jars revealed a bunch of black contam after shaking. Set it aside and let it do its thing so I could watch.

The others looked fine, but I think they may be growing some black specks. I guess I'll find out if they do the same thing as the first one when I break them up to spawn. But I'm curious if yall got any insights.

The community here has done a good job at managing my expectations for mss to grain spawn. But I'd like to do a few shoe boxes if I can get away with it.

These were all knocked up June 29th (13 days)

Here's the obviously contaminated one, the contamination has grown a lot since I first noticed it after the initial shake.



Here's the ones that look decent, but that i suspect of being contaminated








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Offlinetututotutut
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Re: Recognizing and dealing with contamination [Re: Gotfrey]
    #27381926 - 07/10/21 04:25 PM (4 months, 16 days ago)

Gotfrey thats definitely bacterial. Try again!


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