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OfflineLimitline
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Registered: 07/01/21
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Last seen: 2 years, 10 months
New to Agar, numbers of transfer?
    #27370399 - 07/01/21 12:24 PM (2 years, 10 months ago)

Hello shroomery,

I recently came back to this hobby after trying it out already some years ago with PF cakes and a few shoe boxes.

Now finally came around to get started with agar since I had old, half dried up multispore syringes and some prints to start up with.

I made up my own MSS from two different prints and inoculated some agar. Unfortunately I didn't get many plates that actually showed any growth. At least my sterile procedure seems to be alright.
But I got one that looked promising and actually grew over the whole plate and even showed some pins on the sides.
Now I made some transfers to new plates and I'm wondering if this growth is normal and what would be my best next steps.

If there should be more transfers or if I should try to inoculate some grain/90-second rice bags to fruit them.

Here are some pictures:

Original plate I made transfers from:






Edit:
strain is B+ supposedly
sorry for bad pictures my phone was mostly focusing on the container

Edited by Limitline (07/01/21 12:29 PM)

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OnlineBig_Dub
I'm just some guy
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Registered: 01/12/11
Posts: 2,952
Loc: Los Angeles Flag
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Re: New to Agar, numbers of transfer? [Re: Limitline]
    #27371645 - 07/02/21 12:32 PM (2 years, 10 months ago)

Hard to tell with those photos. But I would make another xfer


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Invisiblefungusul
Fungus Kingdom


Registered: 07/16/20
Posts: 1,065
Re: New to Agar, numbers of transfer? [Re: Big_Dub]
    #27375377 - 07/05/21 05:26 PM (2 years, 10 months ago)

:whathesaid:

Pictures are not very good so it's hard to tell. In my opinion, it's good to have at least 4-5 transfers especially if you are a beginner. Once you gain more experience with the identification of contaminants on agar you can go with lower number of transfers.

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