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OfflineSnarfBarf
Stranger Danger
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Registered: 11/03/17
Posts: 29
Loc: Washington, USA
Last seen: 3 months, 23 days
Re: Syringe to Agar [Re: sandman420] * 1
    #27307613 - 05/13/21 11:07 PM (2 years, 8 months ago)

Quote:

JoBloShow said:
I now have a better method than what I stated above to germinate dirty syringes.
I just make small BRF cakes using my agar dishes and shoot a drop of spore solution on them. BRF seems to be far more resilient to bacteria than agar or grain.  I've never had a BRF dish like this not germinate, no matter how bacterial the syringe.
Once you see clean growth, you can transfer a piece to regular agar and continue cleaning like that, or you can even let the plate cake fully colonize and fruit it, or just pluck a pin and clone that.

You're essentially just doing the PF tek (only on a smaller scale) and cloning or transferring myc from there.




Awesome! Have to give that a try. I just noticed my 3rd syringe to agar batch molding up on day 3. My incubator is set to 80, dishes 'are wrapped and upside down', I did everything in a laminar setup. I guess brf cakes will be my 4th batch lmao.

edit in ' ' marks


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Snarf


Edited by SnarfBarf (05/14/21 11:21 AM)


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InvisibleMilk-away
'Mmkay fortyseven
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Registered: 02/04/21
Posts: 249
Re: Syringe to Agar [Re: SnarfBarf]
    #27307682 - 05/14/21 12:41 AM (2 years, 8 months ago)

Yes! This technique worked very well for me with a bacterial MSS.
Just make sure to open the jar and use sterile tools to extract the healthy mycelium out unto some agar as soon as you see healthy growth.


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OfflineSnarfBarf
Stranger Danger
I'm a teapot User Gallery


Registered: 11/03/17
Posts: 29
Loc: Washington, USA
Last seen: 3 months, 23 days
Re: Syringe to Agar [Re: Milk-away]
    #27364121 - 06/26/21 12:44 PM (2 years, 7 months ago)

Great success. While there was major contamination in the jars - maybe they weren't PC'd long enough - healthy grow was able to be carefully removed and transferred to agar. Once the petris were halfway colonozied, healthy rhizo growth was transferred yet again to a new petri, no contams. Further isolation of strains began a few days ago and th latest petris have shown no contams thus far.

Advice on starting from syringe was greatly appreciated.


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Snarf


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