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Invisibleralf
It's all in the mind


Registered: 01/24/13
Posts: 179
Agar with broth from straw instead of potato?
    #27358921 - 06/22/21 02:16 PM (2 years, 10 months ago)

Hello, i'm new to agar.
Looking at potato dextrose agar right now.
Growing oysters and winecaps on fairly old straw currently.
If i let the straw soak for two days the soaking water becomes very yellow.
Looking at potato agar i figured the potato broth serves as nurtient.
I was wondering if i could substitute the potato broth with the yellow soaking water from the straw?
Why wouldn't this work? What could be issues with it? What am i missing?

Glad to hear some opinions. Sorry if this has been asked before. I couldn't find anything with the search.

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InvisibleBaba Yaga
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Registered: 09/13/20
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Re: Agar with broth from straw instead of potato? [Re: ralf]
    #27359381 - 06/22/21 09:02 PM (2 years, 10 months ago)

So you going to use that for oysers / wine caps? Or are you switching to actives for that?
Probably not enough nutrients but hey why don't you just give it a go and run a few plates.

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Invisibleralf
It's all in the mind


Registered: 01/24/13
Posts: 179
Re: Agar with broth from straw instead of potato? [Re: Baba Yaga]
    #27359879 - 06/23/21 09:53 AM (2 years, 10 months ago)

Quote:

Baba Yaga said:
So you going to use that for oysers / wine caps? Or are you switching to actives for that?
Probably not enough nutrients but hey why don't you just give it a go and run a few plates.




I thought about moving to medicinals like lionsmane, chaga, turkeytail and reishi later.

Yeah, i figured i should maybe just give it a go. I'll keep you posted how it went. Then again if it was working well why wouldn't people talk more about it? I guess i'll have to do it for science.

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InvisibleBaba Yaga
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Re: Agar with broth from straw instead of potato? [Re: ralf]
    #27360224 - 06/23/21 02:26 PM (2 years, 10 months ago)

Yes why not, it's easy enough to check it out and have some fun with it and now that I'm thinking about it again, tea agar works on it's own so
maybe......maybe not......maybe not well enough?

I indeed would appreciate an update on this :camping:

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Invisibleralf
It's all in the mind


Registered: 01/24/13
Posts: 179
Re: Agar with broth from straw instead of potato? [Re: Baba Yaga]
    #27360661 - 06/23/21 08:17 PM (2 years, 10 months ago)

tea agar? Now that gets me interested. Off on a search ...

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InvisibleBaba Yaga
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Re: Agar with broth from straw instead of potato? [Re: ralf]
    #27360673 - 06/23/21 08:25 PM (2 years, 10 months ago)

Oh yes it's used to suppress bacterial growth and the mycelium utilizes the nutrients provided by the tea.
I had cubensis and pan mycelium growing on tea agar without adding other nutrients, it's not winning
a prize in terms of speed but it helps giving the myc an advantage over bacteria.

Search tea agar or t-gel, main poster here is Ferather, he did a lot of work with this.

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Invisibleralf
It's all in the mind


Registered: 01/24/13
Posts: 179
Re: Agar with broth from straw instead of potato? [Re: Baba Yaga]
    #27361085 - 06/24/21 04:49 AM (2 years, 10 months ago)

Already figured as much. It's good to know in case you have a really important genetic you need to save.

What's maybe actually more useful for day to day use is the thing i found about "woodchip tea". Sounds genius on paper.

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InvisibleBaba Yaga
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Re: Agar with broth from straw instead of potato? [Re: ralf]
    #27361098 - 06/24/21 05:17 AM (2 years, 10 months ago)

Got a link?

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Invisibleralf
It's all in the mind


Registered: 01/24/13
Posts: 179
Re: Agar with broth from straw instead of potato? [Re: Baba Yaga]
    #27361287 - 06/24/21 09:42 AM (2 years, 10 months ago)

sure. I thought this was interesting and gave me lots of ideas:

https://www.shroomery.org/forums/showflat.php/Number/20486505#20486505

So today i cooked out some sawdust, added a little bit of black tea to the pot, strained it, added maybe half a gram of honey and the agar. Cook, sterilize, pour.
I'll put chaga, king oysters and some regular oysters on it. See how it goes.

I'll do the straw-juice tomorrow.

Edited by ralf (06/24/21 10:01 AM)

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Invisibleralf
It's all in the mind


Registered: 01/24/13
Posts: 179
Re: Agar with broth from straw instead of potato? [Re: ralf]
    #27382364 - 07/10/21 07:56 PM (2 years, 10 months ago)

To come back to this.

The straw juice is kinda meh. The soup from soaking the woodchips though was well liked by the woodlovers. No contam issues on either.
Me thinks the juice from the wood-soak has better nutrition value than the straw. Still have to do comparison to malt extract agar but so far i'd say: the sauce from the straw-soak is inferior to the sauce from the wood-soak. The wood-juice was colonized rather quickly by the oysters. 

Honestly i don't see how contams could be an issue on sterilized wood-juice. If malt-agar isn't much better i could see myself run the wood-water a lot.

Sadly i don't have a cam to show pictures.

Bottom line for me: wood-tea works well and it actually is what you would grow the shroom on later anyways so it will have an easy time adapting from the petri to the actual substrate. Saves some money too since the wood-juice from soaking the wood is a product you have anyways and you usually end up throwing it out.

All that applies to oysters (probably other woodlovers too). Chaga grows on it too but i don't have enough experience with chaga to make a qualified statement about the health of the mycelium of that.

Anyways, have fun experimenting. Give it a shot if you do woodlovers.

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InvisiblebodhisattaMDiscordReddit
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Registered: 04/30/13
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Re: Agar with broth from straw instead of potato? [Re: ralf]
    #27382777 - 07/11/21 05:48 AM (2 years, 10 months ago)

The idea mycelium has to adapt to a substrate is some goofy Paul stamets theory from decades ago. Mycelium "eats" using enzymes. It's capable of producing enzymes encoded by it's DNA and that's it. There's no adaptation to new substrate. It either can eat or cannot. Cellular up-regulation of certain enzymes happens incredibly rapidly when mycelium is exposed to something it needs to break down and can break down.


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