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OfflineTwirl
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Mycelium vs Bacteria (need help)
    #27350972 - 06/16/21 05:45 PM (4 months, 2 days ago)

Hello guys, I'm new to this world of shroom cultivation. Almost two weeks ago I tried to clone some Agaricus bisporus in Agar. A few days ago I noticed some white things that seems to be some bacteria but it's growth stopped, meanwhile Mycelium started to spread from the piece of mushroom. Is this dangerous for the mycelium growing in my agar? Should I grab whatever piece of Mycelium i can and transfer to a new agar plate or Mycelium will eventually colonize and "kill" those white globs of bacterial formation? Any help is appreciated, thanks a lot.



Edited by Twirl (06/16/21 05:49 PM)


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OfflineMycoctopuss
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Re: Mycelium vs Bacteria [Re: Twirl]
    #27350985 - 06/16/21 05:54 PM (4 months, 2 days ago)

even if mycelium can defeat the Bacteria Wich is very common for fungi to beat the bacteria . the bacteria produce EndoSpores which are very very difficult to kill when the bacteria live in this form. even autoclaving at 121C 15 psi cant kill some endospores and your agar plate is never usable because whenever you inoculate something with that agar the endospores from the bacteria it there and when it finds the environmental conditions are good it will germinate again and produce more bacteria. it's better to cut the bacteria as soon as possible and make a transfer from that plate before its late


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Invisiblestubb
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Re: Mycelium vs Bacteria (need help) [Re: Twirl]
    #27350986 - 06/16/21 05:54 PM (4 months, 2 days ago)

Quote:

Twirl said:
Is this dangerous for the mycelium growing in my agar? Should I grab whatever piece of Mycelium i can and transfer to a new agar plate




Yes, and yes.


But, did you clone a button mushroom from the grocery store?

Quote:

RogerRabbit said:
...

You'll never get a quality, good fruiting clone from the grocery store.  They expand their mycelium to the breaking point of senescence before fruiting to prevent competitor mushroom farms from getting the cultures.  When you get to the high end commercial edibles, cultures are expensive, but at $100, you make that back the very first harvest.





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OfflineTwirl
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Re: Mycelium vs Bacteria (need help) [Re: stubb]
    #27350999 - 06/16/21 06:04 PM (4 months, 2 days ago)

Quote:

stubb said:
Quote:

Twirl said:
Is this dangerous for the mycelium growing in my agar? Should I grab whatever piece of Mycelium i can and transfer to a new agar plate




Yes, and yes.


But, did you clone a button mushroom from the grocery store?

Quote:

RogerRabbit said:
...

You'll never get a quality, good fruiting clone from the grocery store.  They expand their mycelium to the breaking point of senescence before fruiting to prevent competitor mushroom farms from getting the cultures.  When you get to the high end commercial edibles, cultures are expensive, but at $100, you make that back the very first harvest.








I did get it from a grocery store hehe. How did you know that? I need to know. Was because the mycelium present is poor quality just by the looks or because of the bacteria being present?


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Invisiblestubb
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Re: Mycelium vs Bacteria (need help) [Re: Twirl]
    #27351014 - 06/16/21 06:20 PM (4 months, 2 days ago)

lol, I just took a guess. I've seen some folks cultivate agaricus bitorquis around here, but I don't know that I've ever seen a bisporus grow log.  Probably because the cultures are so expensive.

No, your tissue transfer looks pretty good to me.  I'd say it's perhaps a little larger chunk than necessary if I had to make some critique.  Bacteria happens, it's everywhere.  A little bacteria especially when taking clones is no big deal at all IMO.  That's one of the many reasons we use agar.  Good job. :thumbup:


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Invisiblestubb
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Re: Mycelium vs Bacteria (need help) [Re: stubb]
    #27351028 - 06/16/21 06:31 PM (4 months, 2 days ago)

Also I got a little ahead of myself in my first response...

You would want to transfer to a new plate before the mycelium and bacteria meet, but I'd let that grow out a little more before transferring to let the mycelium better establish itself.


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