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InvisibleAtomsplit
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Registered: 01/16/21
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Re: Mold on agar? [Re: PitcherCrab]
    #27348013 - 06/14/21 02:34 PM (2 years, 9 months ago)

Hey verum & pitcher!

Thanks for chiming in. All of my T-1 transfers came off of growth like this.

It's the only organized growth I see as the others look disorganized.

I've been reading tons but can find anything that looks like this. Possiblity mold?



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OfflinePitcherCrab
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Re: Mold on agar? [Re: Atomsplit]
    #27348216 - 06/14/21 05:16 PM (2 years, 9 months ago)

The one you took the transfers from is the best of the four. The other three are looking questionable to me in this new photo. I'd like to see how the new transfers grow out.


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear


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InvisibleAtomsplit
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Re: Mold on agar? [Re: PitcherCrab]
    #27350146 - 06/16/21 02:29 AM (2 years, 9 months ago)

Thanks pitch & vs!

Well as a backup I germinated my other spore print. I think this looks better. Any opinions would be appreciated.



I'm just hoping either T-2 of my first germination plate (doesn't look right)

Or these new T-1 transfers off this T-0 plate shown above work out.

Otherwise as I may have to start a pftek and clone off those fruits.

Thanks Shroomery. :sporedrop:


Edited by Atomsplit (06/16/21 02:40 AM)

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OfflinePitcherCrab
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Re: Mold on agar? [Re: Atomsplit]
    #27350381 - 06/16/21 08:51 AM (2 years, 9 months ago)

Man this has been quite the ride for you! That germ plate looks more promising. Please continue to post pictures of your transfers and we'll do our best to advise you. It's good to go to T2-T3 from the germ plate just to be safe. Do you have the means of starting a PF jar if this doesn't work out?


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear


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InvisibleAtomsplit
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Re: Mold on agar? [Re: PitcherCrab]
    #27350401 - 06/16/21 09:12 AM (2 years, 9 months ago)

Thanks for the help Pitcher.

I inoculated 5 brf jars 2 days ago just as a backup and since I had a few syringes around.

This has been a ride to say the least. It's been fun but I feel overwhelmed when nothing has gone smoothly.

Even my first pftek attempt only 3 of 5 jars made it.
Thanks again!
:mushroom2:


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Edited by Atomsplit (06/16/21 10:52 PM)

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OfflinePitcherCrab
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Re: Mold on agar? [Re: Atomsplit]
    #27350408 - 06/16/21 09:16 AM (2 years, 9 months ago)

Yeah man... I wonder what's going on. Seems like if you got vendor prints they'd be cleaner to say the least. I hope this next round produces some results!


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear


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InvisibleAtomsplit
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Re: Mold on agar? [Re: PitcherCrab]
    #27350785 - 06/16/21 02:03 PM (2 years, 9 months ago)

I forgot I already did transfers on a early germination plate of the new spore print.

I think this T-1 already looks "better" than the transfers from the first print.

I could be wrong. I usually am but I'm hopeful.



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Edited by Atomsplit (06/16/21 09:04 PM)

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Offlineverum subsequentis
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Re: Mold on agar? [Re: Atomsplit]
    #27351400 - 06/16/21 10:16 PM (2 years, 9 months ago)

Looks like it'll work out.

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InvisibleAtomsplit
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Re: Mold on agar? [Re: verum subsequentis]
    #27353066 - 06/18/21 02:23 AM (2 years, 9 months ago)

Does this germination plate look like its filled with mold/ contamination?
Thanks in advance.



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Edited by Atomsplit (06/18/21 02:58 AM)

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Offlineverum subsequentis
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Re: Mold on agar? [Re: Atomsplit]
    #27353498 - 06/18/21 11:34 AM (2 years, 9 months ago)

Germ plates are tricky. That looks suspect to me. Only way to find out is a transfer. Could be anything from straight mold to cube myc with a little sumpin sumpin to straight cube myc.

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Re: Mold on agar? [Re: verum subsequentis]
    #27353575 - 06/18/21 12:44 PM (2 years, 9 months ago)

:whathesaid:


--------------------
split_by_nine said:
click me you fuck


do the right thing

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InvisibleAtomsplit
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Re: Mold on agar? [Re: Big_Dub]
    #27355273 - 06/19/21 02:07 PM (2 years, 9 months ago)

I'm stumped. As shown all my germination plates look suspicious. These are off 2 prints from a reliable vendor.

The transfers haven't gone well as shown in this thread.

These are the latest.


Anything I could be missing here?

I went back in again and streaked more plates with a different agar recipe and even used a syringe.

Thank u


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Edited by Atomsplit (06/19/21 02:26 PM)

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Offlineverum subsequentis
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Re: Mold on agar? [Re: Atomsplit]
    #27355599 - 06/19/21 07:34 PM (2 years, 9 months ago)

Looks dirty. Mighta just had shit in it from the beginning. I'd be looking forward to some new spores. Market place might be just what you need.

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OfflinePitcherCrab
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Re: Mold on agar? [Re: verum subsequentis]
    #27355670 - 06/19/21 08:43 PM (2 years, 9 months ago)

I'm with Verum. I believe the spore prints are the issue... Seems like you're doing everything right and still ending up with shit every time.


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear


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InvisibleAtomsplit
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Re: Mold on agar? [Re: PitcherCrab]
    #27355934 - 06/20/21 01:49 AM (2 years, 9 months ago)

Quote:

verum subsequentis said:
Looks dirty. Mighta just had shit in it from the beginning. I'd be looking forward to some new spores. Market place might be just what you need.



Quote:

PitcherCrab said:
I'm with Verum. I believe the spore prints are the issue... Seems like you're doing everything right and still ending up with shit every time.




Thanks Verum, Pitcher & everyone that's helped me on this thread. You guys been great! :mushroom2:

I'll see if the vendor can help but it's past the 30-60 day window.


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Edited by Atomsplit (06/20/21 01:53 AM)

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Offlineverum subsequentis
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Re: Mold on agar? [Re: Atomsplit]
    #27355941 - 06/20/21 01:57 AM (2 years, 9 months ago)

I'd probably go with a different vendor or hit up the market place.

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InvisibleAtomsplit
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Re: Mold on agar? [Re: verum subsequentis]
    #27355954 - 06/20/21 02:09 AM (2 years, 9 months ago)

I've already emailed the vendor. Is that bad practice?
The other vendors don't work for me atm.

One very kind shroomery member has reached out to offer help- he/she had the foresight that the prints may have been the issue.


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Edited by Atomsplit (06/20/21 02:16 AM)

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OfflinePitcherCrab
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Re: Mold on agar? [Re: Atomsplit]
    #27355961 - 06/20/21 02:19 AM (2 years, 9 months ago)

Naw it's fine to email vendors. Most are super helpful and will replace your prints/syringes no questions asked. If you mention you're a shroomery member sometimes they will throw in an extra for free. I hope you are able to get them replaced!


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear


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Offlineverum subsequentis
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Re: Mold on agar? [Re: PitcherCrab]
    #27356650 - 06/20/21 03:41 PM (2 years, 9 months ago)

trying again from the same print but a different place can sometimes fix the issue. Just depends how fucked it is.

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InvisibleAtomsplit
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Re: Mold on agar? [Re: verum subsequentis]
    #27356660 - 06/20/21 03:51 PM (2 years, 9 months ago)

Quote:

verum subsequentis said:
trying again from the same print but a different place can sometimes fix the issue. Just depends how fucked it is.




Thanks VS. Good idea!

Do you mean using another section of the print? Or moving my workspace?

The 2nd print was small so there were two in there. I went to the untouched print and waiting for those to germinate


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Edited by Atomsplit (06/20/21 07:23 PM)

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Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Buy Bali Kratom Powder   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale


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