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OfflineTheFinNewGuy
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Air Exchange Requirements for Slants
    #27343288 - 06/10/21 05:27 PM (2 days, 5 hours ago)

I could use some advice on slants.  I've decided to take the plunge but again, I find conflicting information.  I've seen videos and posts advocating that due diligence be given toward providing slants with air exchange.  I've also seen others recommend the use of vacutainers for slants.  I don't see any way of providing air exchange with those.  What is the current thinking on starting and keeping slants with regard to air exchange?

Thanks.


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OnlineLtLurker
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Re: Air Exchange Requirements for Slants [Re: TheFinNewGuy]
    #27343385 - 06/10/21 06:56 PM (2 days, 3 hours ago)

Most people don't worry about GE for slants. When you first put a wedge in, you need the lid a bit loose and wrapped like a plate so it'll colonize the surface. Once grown you tighten down lid and stick in fridge. At that point i think they're pretty much dormant from the cold and GE isn't an issue for atleast months, possibly years.


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OfflineTheFinNewGuy
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Re: Air Exchange Requirements for Slants [Re: LtLurker]
    #27343603 - 06/10/21 10:10 PM (2 days, 45 minutes ago)

Do these look like reasonable tubes?  They are polypropylene.



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OfflineCocaineBuffet
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Re: Air Exchange Requirements for Slants [Re: TheFinNewGuy]
    #27343611 - 06/10/21 10:19 PM (2 days, 36 minutes ago)

Those are what I use and I do not make any modifications for GE. Just wrap them in parafilm like an agar plate. No issues.

Edit: Wrap the lid to the tube with parafilm with the lid screwed on. Just in case that may not have been clear.


Edited by CocaineBuffet (06/10/21 10:20 PM)


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Re: Air Exchange Requirements for Slants [Re: CocaineBuffet]
    #27344123 - 06/11/21 10:23 AM (1 day, 12 hours ago)

Can they be pressure cooked with the agar in them and lids on loose?


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Re: Air Exchange Requirements for Slants [Re: TheFinNewGuy]
    #27344877 - 06/11/21 10:05 PM (1 day, 50 minutes ago)

I do hot pours with my plates and slants so I am not sure. Maybe another user can chime in regarding PCing slants. As long as the plastic is at least PP5 it should work.

If you wanted to do a no-pour style slant check out the Pasty Plates thread. I would guess that tek could be appropriated for slants.


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Re: Air Exchange Requirements for Slants [Re: CocaineBuffet]
    #27344919 - 06/11/21 10:52 PM (1 day, 4 minutes ago)

Those are PP5 and I PC them. It's basically like no pour or pasty plates.
After making agar i'll fill tubes bout 1/2 way, put the lid on loosely so pressure can escape, and put my slants in a Grain jar for the cycle(keeps them upright).
After cycle, remove and tighten & wrap lids in a SAB, place them tilted toward their side for bigger surface area while cooling.

If you're gonna put a bit of wood(supposed to help sustain longer) you'd need to pc like that.


Edited by LtLurker (06/11/21 11:06 PM)


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