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OfflineAK1000
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How does Bod achieve g2g colonization "in 3-4 days using 1:10" ???
    #27342927 - 06/10/21 12:55 PM (4 days, 14 hours ago)

In one of his teks he goes over timetable/yields/BE and says "g2g is 3-4 days to colonize using 1:10" How is this possible? It takes my jars about 2-3 weeks until they're 100% colonized.


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Re: How does Bod achieve g2g colonization "in 3-4 days using 1:10" ??? [Re: AK1000]
    #27342943 - 06/10/21 01:14 PM (4 days, 14 hours ago)

2-3 weeks is agar wedge times. Maybe your moisture content is off. Maybe Your master jar isn't good


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OfflineRyzuma
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Re: How does Bod achieve g2g colonization "in 3-4 days using 1:10" ??? [Re: AK1000]
    #27342967 - 06/10/21 01:38 PM (4 days, 14 hours ago)

2-3 is kinda long. My g2g takes at least 8 days. more often than not it takes 10.


Edited by Ryzuma (06/10/21 01:39 PM)


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Re: How does Bod achieve g2g colonization "in 3-4 days using 1:10" ??? [Re: Ryzuma]
    #27342972 - 06/10/21 01:43 PM (4 days, 14 hours ago)

Interesting. My master jars are good, those are inoculated with agar. The agar ones definitely take about 3 weeks. The G2G ones using the masters from agar take 2-3 weeks. This isn't the first time I'm doing this.. it has been this way everytime. Maybe I'm just not using enough g2g (maybe more like a 1:15 ratio instead of 1:10).


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Re: How does Bod achieve g2g colonization "in 3-4 days using 1:10" ??? [Re: Ryzuma]
    #27343244 - 06/10/21 04:52 PM (4 days, 10 hours ago)

Quote:

Ryzuma said:
2-3 is kinda long. My g2g takes at least 8 days. more often than not it takes 10.




So how does one achieve 3-4 days?


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Re: How does Bod achieve g2g colonization "in 3-4 days using 1:10" ??? [Re: AK1000]
    #27343248 - 06/10/21 04:55 PM (4 days, 10 hours ago)

stellar genetics from a clone/isolate, perfectly hydrated grains, optimal temps, etc. i’m assuming


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Re: How does Bod achieve g2g colonization "in 3-4 days using 1:10" ??? [Re: AK1000]
    #27343474 - 06/10/21 08:25 PM (4 days, 7 hours ago)

No clue. I've never done it. 8-10 days is what I get with g2g everytime


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Re: How does Bod achieve g2g colonization "in 3-4 days using 1:10" ??? [Re: Ryzuma]
    #27343791 - 06/11/21 02:15 AM (4 days, 1 hour ago)

Genetics seem to be most important. I got AGT from some dude and it will colonize in 4 days with a good shake. It’s super aggressive whereas my B+ I’ve been isolating for the last few months has been brought down to like a week. I’m pretty new to this and I immediately noticed the AGT was way more aggressive so I assume the guy who sent it to me did work with it.


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Re: How does Bod achieve g2g colonization "in 3-4 days using 1:10" ??? [Re: cutie]
    #27343848 - 06/11/21 03:40 AM (4 days, 11 minutes ago)

3-4 days is best case scenario.

Generally speaking i get around 7-8 days colonization but I use low g2g ratios.


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Re: How does Bod achieve g2g colonization "in 3-4 days using 1:10" ??? [Re: DERRAYLD]
    #27343940 - 06/11/21 06:17 AM (3 days, 21 hours ago)

I would say genetics are the least important. Health of your culture, grain prep, g2g ratio, temperature, gas exchange, and even dispersal of thd the colonized grain into the receiving jar I would think are all more important


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Re: How does Bod achieve g2g colonization "in 3-4 days using 1:10" ??? [Re: bodhisatta]
    #27344317 - 06/11/21 01:29 PM (3 days, 14 hours ago)

Quote:

bodhisatta said:
I would say genetics are the least important. Health of your culture, grain prep, g2g ratio, temperature, gas exchange, and even dispersal of thd the colonized grain into the receiving jar I would think are all more important





Interesting. Thanks for answering bod!

The culture itself is very healthy, I have slants that I routinely use to make a shitload of agar plates and then Agar to Grains to make master jars and then use those to G2G for the motherload. So, I'm not sure how else to gauge the health of the culture other than it's fairly fresh and not succumbing to senescence. They colonize the plates fast and pin in 8-10 days in a monotub.

Grain prep, the oats are plump after 30-40mins of boiling and I let them dry out for several hours so the surface is pretty much dry.

G2G ratio I suppose is low, something around 1/15 instead of 1/10.

Temperature, remains around 75-79 degrees these days.

I try to distribute them evenly as well.

In regards to gas exchange, the bottles are sealed up. I've only cracked them open if they seem stalled out after 3 weeks.

So, I could up the G2G ratio and maybe lack of gas exchange is an issue, maybe not.


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Re: How does Bod achieve g2g colonization "in 3-4 days using 1:10" ??? [Re: AK1000]
    #27344874 - 06/11/21 10:03 PM (3 days, 5 hours ago)

if you’re saying they are sealed up like, sealed so that no air gets in or out, that’s your problem. you need constant gas exchange for colonizing jars, mycelium needs to breathe. you gotta use lids with filters on them or plastic lids that are one piece left slightly loose


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Re: How does Bod achieve g2g colonization "in 3-4 days using 1:10" ??? [Re: hazyhorse]
    #27345485 - 06/12/21 01:54 PM (2 days, 13 hours ago)

Gotcha. Thanks!


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