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OfflineWillow28
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Registered: 05/18/21
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Autoclave Tape
    #27343462 - 06/10/21 08:11 PM (4 days, 9 hours ago)

Hi everyone,
Doing PF Tek. The Psilocybin Bible book suggests to put autoclave tape (in an X), over the foil before putting them in the PC. This way when I go to inoculate after sterilization, I can stick the needle through the edge of the autoclave tape opposed to the foil which can tear.

My question is: Should I wipe the needle with alcohol or fire with the lighter after each squirt? I may be OCD, but I just think, what if the glue from the autoclave tape gets on the needle and causes contamination?

Should I heat it up the lighter with each corner of the jar I inoculate? I will be doing 12 half pint jars so that's 4 squirts per jar. That's what the book tells you.

I do have micropore tape too if you think that might be better...

Sigh, thank you for helping me with all this. I don't know what I'd do without this forum.

Best,
Willow


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Invisiblepablokabute
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Re: Autoclave Tape [Re: Willow28]
    #27343468 - 06/10/21 08:20 PM (4 days, 9 hours ago)

The dry verm layer at the top is more than enough to filter contams out.

Id say fire the needle to red hot before EACH squirt.

Also, try to learn agar if youre really that OCD. You'll thank me afterwards. Enjoy. :wink:


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Invisiblefungusul
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Re: Autoclave Tape [Re: pablokabute]
    #27343490 - 06/10/21 08:40 PM (4 days, 9 hours ago)

Welcome to Shroomery! :mushroom2:

While optional, micropore tape(3M) can be added on top of the holes in lid to add an extra filter. After PC, remove the aluminum foil in SAB, and inoculate with a red hot needle through each hole covered by micropore tape. Micropore tape from 3M can be autoclave, but it needs to be removed after each cycle.

Wipe your syringe and needle with alcohol before starting the whole inoculation process of the jars to sanitize them.

Do not wipe with alcohol the needle after it's sterilized with fire. This could bring contaminants on your needle.

In my opinion, sterilizing the needle between each hole is an overkill. It takes a lot of time if you don't have a torch, so I'm sterilizing after each jar.

Read latest PF-TEK Updated PF Tek Guide for more information.


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OfflineBakedbeings
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Re: Autoclave Tape [Re: fungusul]
    #27343575 - 06/10/21 09:45 PM (4 days, 8 hours ago)

if you move your jars directly from your pc to your sab with the foil still on, the lids and verm layer are clean and you can just sterilize the needle once before inoculating as many jars as you can do in a row


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Invisiblepablokabute
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Re: Autoclave Tape [Re: Bakedbeings]
    #27343612 - 06/10/21 10:21 PM (4 days, 7 hours ago)

Quote:

Bakedbeings said:
if you move your jars directly from your pc to your sab with the foil still on, the lids and verm layer are clean and you can just sterilize the needle once before inoculating as many jars as you can do in a row





Its too risky to not flame the needle ATLEAST each jar.


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..


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OfflineBakedbeings
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Re: Autoclave Tape [Re: pablokabute]
    #27343618 - 06/10/21 10:25 PM (4 days, 7 hours ago)

then why not flame between each hole? what are you picking up on the needle between each jar that you wouldnt pick up doing 4 or 5 injections in one? if im starting with a fresh needle i dont flame once


Edited by Bakedbeings (06/10/21 10:26 PM)


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Invisiblepablokabute
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Re: Autoclave Tape [Re: Bakedbeings]
    #27343624 - 06/10/21 10:35 PM (4 days, 7 hours ago)

Quote:

Bakedbeings said:
then why not flame between each hole? what are you picking up on the needle between each jar that you wouldnt pick up doing 4 or 5 injections in one? if im starting with a fresh needle i dont flame once





Not flaming each hole is a bit ok, you can forgo doing so. People avoid it because its a bit inefficient. But it wont hurt your jars, yes its overkill but it will also WORK.

Build a still air box(SAB), it would increase your chances.


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..


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Invisiblepablokabute
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Registered: 11/22/11
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Re: Autoclave Tape [Re: Bakedbeings]
    #27343627 - 06/10/21 10:36 PM (4 days, 7 hours ago)

Quote:

Bakedbeings said:
then why not flame between each hole? what are you picking up on the needle between each jar that you wouldnt pick up doing 4 or 5 injections in one? if im starting with a fresh needle i dont flame once





It might pick-up a single dust in the air, which could potentially harbor contaminant spores.


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..


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OfflineBakedbeings
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Re: Autoclave Tape [Re: pablokabute]
    #27343662 - 06/10/21 11:23 PM (4 days, 6 hours ago)

Quote:

It might pick-up a single dust in the air, which could potentially harbor contaminant spores.




thats why i use a SAB and move quickly


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Invisiblefungusul
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Re: Autoclave Tape [Re: Bakedbeings]
    #27344689 - 06/11/21 07:06 PM (3 days, 10 hours ago)

In my opinion, you need to flame the needle between jars because you could carry contaminants from one contaminated jar to another uncontaminated jar.

You don't need to flame between holes of the same jar because:
- if one part of the jar is contaminated you can safely assume that whole jar is contaminated.
- when you inoculate hole after hole, the travel distance and time with needle in contaminated air is greatly reduced, less air currents created by moves of your hands outside of SAB.
- very inefficient especially if you don't have a good fire source.


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