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OfflineTrippynoodle
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Syringe from non sterile print
    #27340205 - 06/08/21 02:35 PM (6 days, 14 hours ago)

Hello there!
I’m curious, if with everything sterile you were to scrape spores into shot glass and spray a little bit of 3% peroxide on them and then add your sterile water, would this better the chances of a clean syringe? A lot of things have said it kills the spores but I’ve seen great success with peroxide on a swab to agar.


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OfflineBig_Dub
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Re: Syringe from non sterile print [Re: Trippynoodle] * 1
    #27340212 - 06/08/21 02:39 PM (6 days, 14 hours ago)

Spores are just inherently dirty.
Bod made a spore syringe tek which is pretty good

hydrogen peroxide is unnecessary


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Math is life
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OfflineBakedbeings
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Re: Syringe from non sterile print [Re: Big_Dub]
    #27340441 - 06/08/21 05:59 PM (6 days, 11 hours ago)

you might as well just squirt a few drops on agar and go from there


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OfflineBig_Dub
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Re: Syringe from non sterile print [Re: Bakedbeings]
    #27340601 - 06/08/21 08:27 PM (6 days, 9 hours ago)

:whathesaid:


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Math is life
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Invisiblefungusul
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Re: Syringe from non sterile print [Re: Big_Dub] * 2
    #27340626 - 06/08/21 08:41 PM (6 days, 8 hours ago)

Welcome to Shroomery! :mushroom2:

Bod's So Easy A Caveman Could Do It™ Spore Syringe Making TEK for creating a spore syringe is good.

You can always assume that spore prints are dirty and spore syringes are dirtier. The main focus should be on agar techniques. Always use plate streaking techniques like described in c10's Agar Guide.


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OfflineTe0nanacatl
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Re: Syringe from non sterile print [Re: fungusul]
    #27341531 - 06/09/21 01:44 PM (5 days, 15 hours ago)

Possibly a stupid question, but if we always treat spores as dirty, what is a difference between doing a spore print on clean foil under a glass or in a SAB?

I understand it will be cleaner in a SAB, but it will be far from sterile, and I can't see how it would be significantly cleaner than the one under the glass?


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OfflineLtLurker
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Re: Syringe from non sterile print [Re: Te0nanacatl]
    #27341638 - 06/09/21 03:05 PM (5 days, 14 hours ago)

The more you're messing with it in open air the worse the contam. The more you can keep it in a SAB/flowhood and double print, the cleaner your print is. Not sterile sure, but better.

Ya know the 5 second rule for dropping food? sure that sandwich aint clean, but 2 seconds on the floor is cleaner than if it was a day under the couch.


Edited by LtLurker (06/09/21 03:08 PM)


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OfflineTe0nanacatl
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Re: Syringe from non sterile print [Re: LtLurker]
    #27341653 - 06/09/21 03:19 PM (5 days, 14 hours ago)

I agree, but if I rip a piece of foil, put a mushroom cap on it, make a tent (as in Bod's printing tek), cover it with a pint jar, and after 12 hours lift the jar, remove the cap and fold the foil. In that case foil was exposed to open air somewhere between 10 and 30 seconds, inside of a closed, still-air room.

Is difference between that and doing it in a SAB really the same as 2 seconds on floor and a day under the couch?

I understand that it's better, I'm just not sure how much better. Although, it could be possible that I just want an excuse to avoid making a SAB :laugh:


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OfflineBig_Dub
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Re: Syringe from non sterile print [Re: Te0nanacatl]
    #27341657 - 06/09/21 03:21 PM (5 days, 14 hours ago)

Making excuses to avoid making a still airbox takes more effort than just making one  lol

You can take a sporeprint in open air and it's just gonna be a bitch to clean up


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OfflineLtLurker
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Re: Syringe from non sterile print [Re: Te0nanacatl]
    #27341663 - 06/09/21 03:24 PM (5 days, 14 hours ago)

Well 2 points. for one spore prints just aren't perfect. You're going for as clean as possible so oyu fight less contams later. Second, you're missing a key part of the tek, the double print. Your first print foil is trashed and replace to dump the most contaminated spores, this transfer is done in a SAB.

There's no getting around a SAB. Unless you wanna just shoot dirty spores into brf jars in the open air and throw half of'em away later. If you wanna be good at this, you're gonna want atleast a SAB and get into agar.


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OfflineBakedbeings
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Re: Syringe from non sterile print [Re: LtLurker]
    #27341754 - 06/09/21 04:35 PM (5 days, 12 hours ago)

ah yes the ol' "if its not sterile either way whats the point of taking any precautions at all" post

because an ounce of prevention is worth a pound of cure. when you put those spores on agar you will see the difference between having to do several transfers to clean them up or maybe not having to do any at all, the choice is yours!

the SAB is incredibly effective considering how simple it is. try doing g2g transfers in open air


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OfflineTe0nanacatl
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Re: Syringe from non sterile print [Re: Bakedbeings]
    #27342305 - 06/10/21 01:06 AM (5 days, 4 hours ago)

I understand.

The thing is, up to this point, I didn't have any major investments. I only bought some shoeboxes, coco coir and verm. Now I have to make a choice if I want to go in deeper, in which case I have to buy a PC, SAB, dehydrator, Petri dishes, scalpel and inoculation loops, so that I can start working with agar. I don't have that much spare money lying around, so I guess I have to choose do I want to do this as a hobby or no.


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OfflineBakedbeings
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Re: Syringe from non sterile print [Re: Te0nanacatl]
    #27342662 - 06/10/21 10:11 AM (4 days, 19 hours ago)

yea its pretty much like any hobby in that you have to buy the stuff in order to do the thing, but PF tek is perfect if you dont want to jump in with both feet yet. you dont need a PC to do it, or agar or petri dishes, but if want to make your own prints a SAB is crucial. luckily its a small investment - just a clear plastic tub and a few minutes of work


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OnlineDERRAYLD
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Re: Syringe from non sterile print [Re: Bakedbeings]
    #27342673 - 06/10/21 10:17 AM (4 days, 19 hours ago)

Research brf and pf style grows or rice, my first mushrooms were grown on white rice.


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OfflineLtLurker
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Re: Syringe from non sterile print [Re: DERRAYLD]
    #27342785 - 06/10/21 11:30 AM (4 days, 17 hours ago)

You can ride out pf tek to bulk for a bit. For that you have most of it cept the SAB and Dehydrator(both cheap).

When you wanna do agar and grains you can watch ebay for a cheap used pc(most expensive piece you need for grains). Slowly add the other stuff over time till you collect everything you need, each individual item is relatively cheap.


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