Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals CBD Concentrates   Mushroom-Hut Substrate Bags   PhytoExtractum Kratom Powder for Sale   North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom   OlympusMyco.com Olympus Myco Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Autoflowering Cannabis Seeds

Jump to first unread post Pages: 1
InvisibleCreonAntigone
Stranger

Registered: 05/30/21
Posts: 2,971
Proper indoor cold-lover technique? (Ps. Semilanceata)
    #27341323 - 06/09/21 09:03 AM (2 years, 11 months ago)

I've made it a project of mine to try and see if I can fruit liberty caps. I know only a few have ever succeeded so I may never do it, but it's a fun process.

I germinated my first P. Semilanceata spores in a terrarium with grass seeds. I saw a lot of good signs of mycelium growth but it never fruited. I tried coldshocking in my fridge at various lengths of time. Coldshocking for less than a day didn't do anything, but I did notice the myc get vigorous and cover the casing layer (peat moss on top of the grass) after a cold shock of 1-4 days. At 1 week or more of cold shock, the myc returned to a more dormant state. Once after a 3-day coldshock I saw a primordia, but it disappeared overnight as the temperatures returned to normal outside my fridge.

So the cold-shock is definitely effective, but I need some way to keep temperatures low and within its preferred fruiting range (<15c-10c) after the cold shock. I will probably have to construct some kind of ice-box  or other temperature-regulated chamber to keep temperatures consistent during fruiting. I wanted to ask what you all thought the best TEK for that kind of temperature-regulation would be. Since it has a similar fruiting season as Cyanescens I figure similar techniques could work. I am not sure if it would be better to stay at a consistent temperature within that range or fluctuate it to mimic a night-day cycle. The myc seems to especially react to temperature changes. I have considered just trying fruiting by regularly taking it in and out of the fridge.

Also, as my sterile technique improves from my first project I am working on germinating a new spore print from this species on agar and doing isolation. So my next grow may go better just by virtue of improved methods.

Extras: Filter Print Post Top
OfflineDINKLB3RG
Myco-Breeder
 User Gallery


Registered: 06/03/21
Posts: 15
Loc: California
Last seen: 1 year, 10 months
Re: Proper indoor cold-lover technique? (Ps. Semilanceata) [Re: CreonAntigone]
    #27341338 - 06/09/21 09:15 AM (2 years, 11 months ago)

Not going to be the most in-depth response as I dont have much experience with species that need cold shock.

But I would try checking out cordy fridge designs and start there. more specifically the ones made out larger wine fridges or kegerators for size. you could probably fit a few 10*20 trays in them just double check internal dimensions. you should be able to regulate the temp fairly easily to your desired ranges.


--------------------

Extras: Filter Print Post Top
OfflineZwinst
Stranger
 User Gallery

Registered: 02/27/21
Posts: 142
Loc: Germany
Last seen: 1 day, 17 hours
Re: Proper indoor cold-lover technique? (Ps. Semilanceata) [Re: DINKLB3RG]
    #27346342 - 06/13/21 03:41 AM (2 years, 10 months ago)

I am planning on doing pretty much the same. :cool:
Mycelium growth is already strong on straw/grass seeds.

One of my ideas is, to put some proper soil on top and let grass grow on it.
Since it is so hard to get them to fruit, i thought maybe there is some weird mycorrhiza stuff going on. Or maybe bacteria that live with the grass? Worth a try at least.


I will probably buy a dedicated fridge some time this year and try cold-shocking some woodlovers too.

Extras: Filter Print Post Top
InvisibleCreonAntigone
Stranger

Registered: 05/30/21
Posts: 2,971
Re: Proper indoor cold-lover technique? (Ps. Semilanceata) [Re: Zwinst]
    #27350791 - 06/16/21 02:07 PM (2 years, 10 months ago)

Let me know how it goes! I've already been trying the grass thing and working to get the mycelium inoculated into healthy grass plants.

It seems to prefer large perennial grasses. This is because those grasses naturally become dormant during the cold. My guess is, the myc is strongest when the plant is dormant because then it can just control the roots fully, and that is when it fruits. So I'd suggesting finding grasses it is known to colonize and working with those.

Options potentially include: Sedges. Easy to germinate native grasses. I grew the myc well in association with Carex typhina. Anything in that Carex genus should work though and be relatively easy to use.

Other options for grasses are discussed in the journal article, "Colonization by Psilocybe semilanceata of roots of grassland flora", which finds that

Quote:

In co-culture in vitro Psilocybe semilanceata invaded moribund cells at the periphery of the cortex of roots of the grass species Agrosiis tenuis, Poa annua and Lolium perenne, and the dicotyledon Stellaria media ...  Colonization of L. perenne was less frequently observed than of the other three species and papilla formation was not observed. P. semilanceata was restricted to the outermost cortical cells of the L. perenne roots by a layer of cells which appeared to contain polyphenolic substances.




So it invaded all those species of grass, but it invaded Lolium a bit less consistently. However, it sounds like lolium should still work somewhat. If the grass seed you have is lolium, you could try just germinating some of those in some soil you inoculated with mycelium.

By the way, where do you get your grass seeds? I've been looking for a good source.

Extras: Filter Print Post Top
OfflineZwinst
Stranger
 User Gallery

Registered: 02/27/21
Posts: 142
Loc: Germany
Last seen: 1 day, 17 hours
Re: Proper indoor cold-lover technique? (Ps. Semilanceata) [Re: CreonAntigone]
    #27351518 - 06/16/21 11:42 PM (2 years, 10 months ago)

Interesting paper!

It seems, that they just eat the dead tissue surrounding the roots instead of entering into a mycorrhiza relationship. Which is a pretty common behavior for lots of mushroom.

Maybe, just maybe they are attracted to bacteria (like rhizobia) that cooperate with the grasses and eating the dead tissue is just a bonus?
Or maybe it is a ectomycorrhiza of some kind. Which is kind of unlikely i think, since P. Sem. has been observed maturing on dung and other living-grass-free substrates. I have never heard of a mycorrhiza forming mushroom that matures without it. I could be wrong though!
Since the mycelium seems to love straw, hey and grass seeds, i would still lean on the side of saprophytic behavior or bacteria being the attractor.

We will see.



I got my grass seeds from the hardware store. The cheapest stuff possible. No idea what is actually in there.
But i will look into acquiring the proper species for experimentation.

Extras: Filter Print Post Top
InvisibleCreonAntigone
Stranger

Registered: 05/30/21
Posts: 2,971
Re: Proper indoor cold-lover technique? (Ps. Semilanceata) [Re: Zwinst]
    #27351945 - 06/17/21 08:47 AM (2 years, 10 months ago)

Quote:

Zwinst said:

Since the mycelium seems to love straw, hey and grass seeds, i would still lean on the side of saprophytic behavior or bacteria being the attractor.





I think this is right. I'm not sure about the technical mycology definitions of what constitutes a mycorrhizal fungus. I think P. Semi is definitely a saprophyte, it just happens to have grass roots as its niche. I've learned that it is basically pathogenic/infectious. It enters the roots and eventually if the infection is really bad it takes over the whole plant, growing hyphae even out of the stems and flowers. It seems to eat the plant material from the inside-out. I think it likes perennial plants because those can actually die once a season.

I've asked myself before whether I think it is good for the plants or bad for them. It seems to have the potential to be good or bad depending on the circumstances. It does compete positively with root rot fungus according to a study I read, and makes its own anti-fungal compounds to help it compete. I've actually found that cultures of this species are rarely susceptible to fungal contamination, even on agar. However they are very susceptible to bacterial contamination and some of my grass grows got quite bacterial because the moisture level was too high.

Though it hurts the plants it seems beneficial for them if they are already struggling. I've found it can do just fine even if the plant is basically nearly dead. It can take over wilting plants and keep them alive almost artificially. It also makes the plant more cold-resistant - I managed to keep one of my plants inside the fridge for a whole week and it only was hurt a little. Once I tried flooding my grass terrarium to see what the myc would do. After that the grass got yellow and wilting but didn't die, and there were air bubbles concentrated around the grass roots. At its full strength the plant is almost a zombie, alive in a weakened state but really only being kept up so the fungus can keep feeding.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Bridgetown Botanicals CBD Concentrates   Mushroom-Hut Substrate Bags   PhytoExtractum Kratom Powder for Sale   North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom   OlympusMyco.com Olympus Myco Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Autoflowering Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Indoor cold loving species experiment SThomas 1,448 1 12/30/01 12:51 AM
by SThomas
* p. azurescens: How? jekojeko 2,416 7 07/16/02 06:49 PM
by DarkTranquility
* Morels at home indoors Peterthinks 2,583 14 11/26/04 08:52 PM
by Prisoner#1
* P. semilanceata
( 1 2 all )
Fungusmaximus 3,590 22 01/14/03 07:56 AM
by deanofmean
* List of cold safe species? srivatsa 1,351 6 12/24/01 11:58 AM
by fuzzysquirelnuts
* P.Azurescens Cultivation Question MrSleep 1,620 8 08/01/03 10:59 AM
by MrSleep
* P. hispanica grow TEK
( 1 2 all )
Zanti 6,745 21 06/13/03 04:31 AM
by Ryche Hawk
* Re: I maybe too high, but could this maybe allow P. Azurescens to be cultivated indoors? Prellgott 2,841 12 12/01/99 11:24 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
622 topic views. 0 members, 2 guests and 5 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.007 seconds on 14 queries.