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OfflineEnDelfisE
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Not very experienced with isolating good hens on agar, sectoring suggestions?
    #27341138 - 06/09/21 05:58 AM (2 years, 11 months ago)

I've been working with many agar plates this past months but I'm always not very sure which point of growth I should transfer

What do you guys think about these two?  I guess it's a good sign they are knotting ey? Should I transfer from the far end where I see most knotting activity?


Edited by EnDelfisE (06/09/21 06:00 AM)

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Invisiblepablokabute
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Re: Not very experienced with isolating good hens on agar, sectoring suggestions? [Re: EnDelfisE]
    #27341148 - 06/09/21 06:09 AM (2 years, 11 months ago)

Left for too long to tell which segments to take, but Im also inexperienced.

Id leave it for a couple more days and just take the good-looking pins if it were mine.


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OfflineEnDelfisE
Rider On The Storm
I'm a teapot User Gallery

Registered: 09/07/12
Posts: 499
Loc: Temple of Demetra
Last seen: 18 days, 11 hours
Re: Not very experienced with isolating good hens on agar, sectoring suggestions? [Re: pablokabute]
    #27341166 - 06/09/21 06:31 AM (2 years, 11 months ago)

Quote:

pablokabute said:
Left for too long to tell which segments to take, but Im also inexperienced.

Id leave it for a couple more days and just take the good-looking pins if it were mine.




Yeah I figured I was a little too late with these :p

I saw some older posts with some people saying that getting pins out of agar didn't yield them the best result, I'm have no experience on the matter so I Can't say if it's for the better or not

By the way do you think having a monocalture or let's say 2-3 difetent genetics would be best? Is there a definite answer on this one?

Edited by EnDelfisE (06/09/21 06:32 AM)

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