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OfflineMycoctopuss
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Tissue culture from fruiting body dikaryon or monokaryon
    #27340360 - 06/08/21 02:46 PM (2 years, 11 months ago)

Hi Dear Experts
I'm a newbie guy who is attending to learning mushroom life cycle and breeding techniques.
in my understanding, the mycelium has a haploid (monokaryon)Structure. and when 2 hyphae meet each other the mushroom sex has happened and the dikaryotic mycelium has been formed and continues to grow as primordia formation and fruiting body has been shaped.
my question is
The fruiting body is dikaryotic. if we Clone a fruiting body to the agar plate the mycelia from this tissue are monokaryotic or dikaryotic? 
we also know for the formation N+N we need 2 monokaryotic mycelia to meet. what happened if 2 dikaryotic mycelia have been met?

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Re: Tissue culture from fruiting body dikaryon or monokaryon [Re: Mycoctopuss]
    #27340930 - 06/08/21 11:38 PM (2 years, 11 months ago)

If you take a tissue sample from a fruiting body it will produce a dikaryotic culture.

Two dikaryons can join through the process called anastomosis.


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OfflineMycoctopuss
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Re: Tissue culture from fruiting body dikaryon or monokaryon [Re: inski]
    #27341033 - 06/09/21 02:24 AM (2 years, 11 months ago)

many thanks for your response
so dikaryotic mycelium can be used for breeding or not?
I see breeders always trying to isolate haploid samples to breed them. why they don't use dikaryotic mycelium for breeding?
many thanks for your information

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OfflineZwinst
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Re: Tissue culture from fruiting body dikaryon or monokaryon [Re: Mycoctopuss]
    #27346347 - 06/13/21 03:46 AM (2 years, 10 months ago)

Quote:

Mycoctopuss said:why they don't use dikaryotic mycelium for breeding?




Because fusion of spores or other mycelia with the dikaryotic mycelium is rare. To get reliable results and to get any results in a timely manner you always want to go with mono.

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OfflineMycoctopuss
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Re: Tissue culture from fruiting body dikaryon or monokaryon [Re: Zwinst]
    #27350882 - 06/16/21 02:50 PM (2 years, 10 months ago)

I have a monotub of apes in the fruiting stage. can I use my own spore swap/print to make fusions and breed my own special ape phenotype?

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InvisiblebodhisattaMDiscordReddit
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Re: Tissue culture from fruiting body dikaryon or monokaryon [Re: Mycoctopuss]
    #27353752 - 06/18/21 02:38 PM (2 years, 10 months ago)

Quote:

Mycoctopuss said:
many thanks for your response
so dikaryotic mycelium can be used for breeding or not?
I see breeders always trying to isolate haploid samples to breed them. why they don't use dikaryotic mycelium for breeding?
many thanks for your information



They're not. Every cross that's circulating right now was from crossing dikaryotic mycelium. No one I've seen yet has produced monokaryotic mycelium and mated it but there are a few stories with no pictures or evidence how convenient


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OfflineZwinst
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Re: Tissue culture from fruiting body dikaryon or monokaryon [Re: bodhisatta]
    #27353825 - 06/18/21 03:35 PM (2 years, 10 months ago)

Very interesting information.

So fusion of dikaryotik mycelia isn´t that rare after all? I just remembered what i read about it and folklore seems to be that it shouldn´t work reliably.

I shall investigate and ask people who might know more.

/edit: And i will try it myself. Just chug 2 different cubes together? I can do that.

Edited by Zwinst (06/18/21 03:37 PM)

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OfflineMycoctopuss
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Re: Tissue culture from fruiting body dikaryon or monokaryon [Re: bodhisatta]
    #27354697 - 06/19/21 02:11 AM (2 years, 10 months ago)

Quote:

bodhisatta said:
Quote:

Mycoctopuss said:
many thanks for your response
so dikaryotic mycelium can be used for breeding or not?
I see breeders always trying to isolate haploid samples to breed them. why they don't use dikaryotic mycelium for breeding?
many thanks for your information



They're not. Every cross that's circulating right now was from crossing dikaryotic mycelium. No one I've seen yet has produced monokaryotic mycelium and mated it but there are a few stories with no pictures or evidence how convenient



many thanks, bodhi
can you kindly explain to us how we can breed mushrooms and creating a stable line of new shrooms for selling our own spore prints and swabs?
edit: is there  any books. resource , course or Etc.. for doing that ?

Edited by Mycoctopuss (06/19/21 02:14 AM)

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InvisiblebodhisattaMDiscordReddit
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Re: Tissue culture from fruiting body dikaryon or monokaryon [Re: Mycoctopuss]
    #27354806 - 06/19/21 05:43 AM (2 years, 10 months ago)

You're already on the resource

https://www.shroomery.org/forums/search.php


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OfflineMycoctopuss
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Re: Tissue culture from fruiting body dikaryon or monokaryon [Re: bodhisatta]
    #27356020 - 06/20/21 04:39 AM (2 years, 10 months ago)

if I want to be a grower .these place completely fulfill me, but actually, I could not find any reliable resources on shroomery or other forums as well about the breeding and the true breeding of the mushrooms

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Re: Tissue culture from fruiting body dikaryon or monokaryon [Re: Mycoctopuss]
    #27358940 - 06/22/21 02:42 PM (2 years, 10 months ago)

For breeding consult the books by the greatest mycologist to ever live; ST Chang.  He has consise breeding information in most of his books, but the books geared toward breeding are the best -just need a basic understanding in basic biology terminology.  I discovered those in this section and actually went on to purchase hardcopies.

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OfflineMycoctopuss
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Re: Tissue culture from fruiting body dikaryon or monokaryon [Re: trippleblack]
    #27359011 - 06/22/21 03:29 PM (2 years, 10 months ago)

thanks bro. can you mention the book name or details? or can you share it with us  ?

Edited by Mycoctopuss (06/22/21 03:32 PM)

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Re: Tissue culture from fruiting body dikaryon or monokaryon [Re: Mycoctopuss]
    #27359121 - 06/22/21 05:02 PM (2 years, 10 months ago)

i would start with mushrooms SECOND EDITION for basics, if you want to get deeper get genetics and breeding of edible mushrooms. in the bhodi library, concise basics is a good start. find good lab technique in google scholar papers -when you know the right key words.

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Re: Tissue culture from fruiting body dikaryon or monokaryon [Re: Zwinst]
    #27399373 - 07/23/21 03:59 PM (2 years, 9 months ago)

Quote:

Zwinst said:
Very interesting information.

So fusion of dikaryotik mycelia isn´t that rare after all? I just remembered what i read about it and folklore seems to be that it shouldn´t work reliably.

I shall investigate and ask people who might know more.

/edit: And i will try it myself. Just chug 2 different cubes together? I can do that.




I have done some reading and after all, find out you both right.
dikaryon mycelium has lost the tendency to exchange Genetic material throughout anastomosis (fusions). so you can see the anastomosis under the microscope but transferring genetic material won't happen most of the time for most Strains
but it is also possible you have done a successful cross with dikaryotic cultures

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