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OfflineSYF8
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Preferred Longterm Genetics Storage * 1
    #27333182 - 06/02/21 09:49 PM (2 years, 8 months ago)

I’m wanting to move on from my current grows and keep the genetics that have been honed in on with past work.  I’ve done the research on cold storage slants, plates and LCs.  Slants seem like the obvious choice, and LCs sound like the tempting edgy choice.  I can do all of them and understand contamination risks.

What is your preferred method?  I want to keep my work and there is really no timeline to start again on varieties focused on in the past.  I prefer the living library approach.  Any and all of these items will be kept in the myco fridge.
Slants
You may choose only one
Wrapped Plates
You may choose only one
LCs
You may choose only one
Go fuck yourself
You may choose only one


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InvisibleSpaceCube
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Registered: 06/02/21
Posts: 153
Re: Preferred Longterm Genetics Storage [Re: SYF8]
    #27333189 - 06/02/21 09:58 PM (2 years, 8 months ago)

I'd just vaccume seal a spore print.

Spore print, in ziplock, then vaccume seal that.


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OfflinePitcherCrab
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Re: Preferred Longterm Genetics Storage [Re: SpaceCube]
    #27333199 - 06/02/21 10:14 PM (2 years, 8 months ago)

I like slants or a clean LC in a vacutainer. Plates are okay for medium term storage.


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OfflineSYF8
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Re: Preferred Longterm Genetics Storage [Re: SpaceCube]
    #27333201 - 06/02/21 10:21 PM (2 years, 8 months ago)

Quote:

SpaceCube said:
I'd just vaccume seal a spore print.

Spore print, in ziplock, then vaccume seal that.




Spore print is what I’m trying to avoid here


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InvisibleAshtray161
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Re: Preferred Longterm Genetics Storage [Re: SpaceCube]
    #27333219 - 06/02/21 10:53 PM (2 years, 8 months ago)

Quote:

SpaceCube said:
I'd just vaccume seal a spore print.

Spore print, in ziplock, then vaccume seal that.




Why would you vaccume deal a sporeprint? Seems like overkill to me


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InvisibleBaba Yaga
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Re: Preferred Longterm Genetics Storage [Re: Ashtray161] * 1
    #27333240 - 06/02/21 11:38 PM (2 years, 8 months ago)

I started to make dehydrated grain spawn from clean jars, just took a few grains out and put in a petri dish and let dry out.
Was able to revive 8 month old grain spawn last year by simply dropping a grain on agar. My oldest ones are 16 month now and I will do another
trial in a few month. Dropping some to agar again and some into LC broth to figure out which way is faster, going from LC to agar of course once
growth is showing. I think I made dehydrated grain spawn of everything I got now and will be assessing this method over the next years.

Here is a link to my post, this will be updated once I got more results:
https://www.shroomery.org/forums/showflat.php/Number/26943501#26943501

I also took some wedges to small containers with distilled water but they are only 3 month old. They are kept at room temperature.

Reason for trying all this is that I want to get away from fridge storage as some species like pans and other tropics don't do that well
in cold storage. At least for me they don't and being not dependent on a fridge is a great incentive for me.

Psilocybin wood lovers and most medicinals and gourmet cultures survive easily 2 years on a regular petri dish in the fridge IME but if I would
have to choose only one method from your list I would go with LC, got some 100ml media bottles that would be perfect for this.


Edited by Baba Yaga (06/02/21 11:50 PM)


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OfflineSYF8
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Re: Preferred Longterm Genetics Storage [Re: Baba Yaga]
    #27334428 - 06/03/21 09:24 PM (2 years, 8 months ago)

I had not heard of tropicals doing poorly in cold storage


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OfflineLtLurker
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Re: Preferred Longterm Genetics Storage [Re: SYF8]
    #27334432 - 06/03/21 09:27 PM (2 years, 8 months ago)

Me neither, i don't think it's actually a thing.

Slants all the way man and go fuck yourself :lol:.


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InvisibleBaba Yaga
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Re: Preferred Longterm Genetics Storage [Re: LtLurker]
    #27334499 - 06/03/21 10:45 PM (2 years, 8 months ago)

For me cubes are good for a year on plates and longer on slants,
oysters, turkey tail, reishi, ps. cyan, ps.semilanceata and such survive much longer but
whenever I put pans in the fridge they are dead after a few month,
maybe I do something wrong :shrug:


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InvisibleViolet
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Re: Preferred Longterm Genetics Storage [Re: Baba Yaga]
    #27334504 - 06/03/21 10:51 PM (2 years, 8 months ago)

Master culture slant tubes. I’ve revived cultures from 4 year old tubes thanks to the wooden sticks when the growth from the agar didn’t look solid. They don’t dry out like dishes, and for the first year+ you can just pluck out a small piece to get a grow going.


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OfflineLtLurker
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Re: Preferred Longterm Genetics Storage [Re: Baba Yaga]
    #27334505 - 06/03/21 10:51 PM (2 years, 8 months ago)

I would try again. I think my Tamp clone is past a year old now and still fine. I can't remember anyone in the exotics or stone maker's threads having issues with slants like that. Maybe if i'm missing something someone will chime in, or you could double check with the guys in the exotics thread. Hell, might even be a trick to it with Pans that could help ya.


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Invisibleelpico
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Re: Preferred Longterm Genetics Storage [Re: LtLurker] * 2
    #27334536 - 06/03/21 11:39 PM (2 years, 8 months ago)

Pan mycelium is fragile, and not very tolerant of cold.  I've lost plenty of pan cultures that were stored in the fridge for less than a year. You have to be vigilant and baby them.  I've used slants for a long time, but find them a pain in the ass to make and store.  I have too many cultures in storage at this point, so I've begun using alternative, more practical storage methods.  I've found that distilled water at room temperature works great for many species.  Here is a 2 year old pan clone culture, and a five year old mexicana MS culture stored in 15 ml centrifuge tubes at room temperature. I shook them, went in with a sterile swab, and dropped the swab to agar: 



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InvisibleAmbrose

Registered: 01/16/20
Posts: 518
Re: Preferred Longterm Genetics Storage [Re: elpico]
    #27334603 - 06/04/21 12:39 AM (2 years, 8 months ago)

Damn, that sounds great. How do you inoculate it? Is it as simple as dropping an agar wedge to distilled water or does it require something more?


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InvisibleBaba Yaga
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Re: Preferred Longterm Genetics Storage [Re: LtLurker] * 1
    #27334619 - 06/04/21 01:07 AM (2 years, 8 months ago)

:whathesaid:


@LtLurker
Yeah maybe there is a way to make it work but I really try to store everything at room temperature and long term without too much hassle.
Slants are great but I am with elpico on this one, it's too much work for my taste and I'm reluctant to buy and run a second fridge which I would
have to do if using slants for all my stuff.


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InvisibleSpaceCube
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Registered: 06/02/21
Posts: 153
Re: Preferred Longterm Genetics Storage [Re: Baba Yaga]
    #27334855 - 06/04/21 06:54 AM (2 years, 8 months ago)

Double Vaccumed Seal Spore Print is the way to go.

Then make spore syringes! PF Tek > Mono.


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Invisibleelpico
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Re: Preferred Longterm Genetics Storage [Re: Ambrose] * 2
    #27335006 - 06/04/21 09:08 AM (2 years, 8 months ago)

I just cut up three or four small wedges and toss them in about 10 ml of distilled water.  Vigorous shake and done.  I like to wrap the cap w/ parafilm or saran wrap.


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Offlineverum subsequentis
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Re: Preferred Longterm Genetics Storage [Re: elpico]
    #27335026 - 06/04/21 09:36 AM (2 years, 8 months ago)

Slants and master plates in cold storage.


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Invisiblenosf3r4tu


Registered: 03/26/19
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Re: Preferred Longterm Genetics Storage [Re: verum subsequentis] * 1
    #27335070 - 06/04/21 10:04 AM (2 years, 8 months ago)

I want to try distilled water storage. Very similar to what elpico suggested. 90% distilled/sterilized water + 10% LC.


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OfflineLtLurker
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Re: Preferred Longterm Genetics Storage [Re: Baba Yaga]
    #27335089 - 06/04/21 10:17 AM (2 years, 8 months ago)

Quote:

elpico said:
Pan mycelium is fragile, and not very tolerant of cold.  I've lost plenty of pan cultures that were stored in the fridge for less than a year. You have to be vigilant and baby them.  I've used slants for a long time, but find them a pain in the ass to make and store.  I have too many cultures in storage at this point, so I've begun using alternative, more practical storage methods.  I've found that distilled water at room temperature works great for many species.  Here is a 2 year old pan clone culture, and a five year old mexicana MS culture stored in 15 ml centrifuge tubes at room temperature. I shook them, went in with a sterile swab, and dropped the swab to agar: 





Quote:

Baba Yaga said:
:whathesaid:


@LtLurker
Yeah maybe there is a way to make it work but I really try to store everything at room temperature and long term without too much hassle.
Slants are great but I am with elpico on this one, it's too much work for my taste and I'm reluctant to buy and run a second fridge which I would
have to do if using slants for all my stuff.



Hey thank you both for teaching me something new here.


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OfflineSYF8
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Re: Preferred Longterm Genetics Storage [Re: LtLurker]
    #27335109 - 06/04/21 10:31 AM (2 years, 8 months ago)

I like the idea of distilled water storage.  I'm going to try that as well as the slants. 

Anyone else do the distilled?  Wedge or LC, seems to me it's very similar.


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