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PreparationH
apply daily

Registered: 03/28/05
Posts: 18,335
Loc: Amsterdam
Last seen: 33 minutes, 43 seconds
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Re: Cultivation General Discussion [Re: Feasoghorm]
#27333166 - 06/02/21 09:31 PM (2 years, 8 months ago) |
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: PreparationH]
#27333180 - 06/02/21 09:48 PM (2 years, 8 months ago) |
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Make sure you use cold water so the agar doesn't clump, and give it a good shake to mix it b4 pc'ing.
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SpaceCube
π

Registered: 06/02/21
Posts: 153
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Re: Cultivation General Discussion [Re: Feasoghorm]
#27333192 - 06/02/21 10:01 PM (2 years, 8 months ago) |
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I'm about to start a Lions Mane grow.
-------------------- π 3.14
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PreparationH
apply daily

Registered: 03/28/05
Posts: 18,335
Loc: Amsterdam
Last seen: 33 minutes, 43 seconds
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Re: Cultivation General Discussion [Re: SpaceCube]
#27333210 - 06/02/21 10:38 PM (2 years, 8 months ago) |
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All done, let it come down to 0psi before pulling it out
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Yeetusdeetus



Registered: 11/23/19
Posts: 1,242
Last seen: 2 days, 21 hours
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Re: Cultivation General Discussion [Re: sandman420]
#27333228 - 06/02/21 11:07 PM (2 years, 8 months ago) |
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Quote:
sandman420 said: I always thought a rocket stove might do the trick. Maybe some kind of adjustable rack to get where you can crank it where you want it, I bet it could be done. Lots of sticks around to burn.
I’ve seen a pressure cooker used over an open flame once. The guy just had a wire rack on top of a couple concrete blocks to hold the pc up. A metal sheet was held over the fire to drop the pressure occasionally
--------------------

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PreparationH
apply daily

Registered: 03/28/05
Posts: 18,335
Loc: Amsterdam
Last seen: 33 minutes, 43 seconds
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Re: Cultivation General Discussion [Re: Yeetusdeetus]
#27333243 - 06/02/21 11:42 PM (2 years, 8 months ago) |
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Done, thanks guys 

Got 19 plates before it solidified in the jar and I couldn't hit that last 20th plate lol.
I will keep one to the side as a control, but I've been doing this long enough I am confident in what I just did. Game changing.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,825
Loc: Gaming the system
Last seen: 7 hours, 5 minutes
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Re: Cultivation General Discussion [Re: PreparationH]
#27333688 - 06/03/21 09:39 AM (2 years, 8 months ago) |
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Some of you guys with a million tubs need one of these
https://newatlas.com/robotics/mushroom-picking-robot/
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LAGM2020     
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Camera93
We got dicks like Jesus



Registered: 08/15/18
Posts: 3,244
Last seen: 1 hour, 35 minutes
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Re: Cultivation General Discussion [Re: A.k.a] 1
#27333691 - 06/03/21 09:45 AM (2 years, 8 months ago) |
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Quote:
A.k.a said: Some of you guys with a million tubs need one of these
https://newatlas.com/robotics/mushroom-picking-robot/
lmao I get an eco earth coco ad in the middle of the article 
-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
Edited by Camera93 (06/03/21 09:45 AM)
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Vinci
I'm You



Registered: 08/26/19
Posts: 639
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Re: Cultivation General Discussion [Re: Camera93]
#27333733 - 06/03/21 10:39 AM (2 years, 8 months ago) |
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What y'all think about putting a blender lid on a fresh LC right after an agar drop and blending your wedge into the LC? Think it might speed the LC colonization?
-------------------- Vinci luh you
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Cultivation General Discussion [Re: Vinci]
#27333737 - 06/03/21 10:45 AM (2 years, 8 months ago) |
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It would if done right. I don't see a need though. LC on a stir plate is quick enough for me.
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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I see now why it's advised to add some broken glass or how yall use stir plates like real scientists
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Feasoghorm]
#27333821 - 06/03/21 12:08 PM (2 years, 8 months ago) |
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You let that happen 
Having something in the jar to break up the colony is useful for sure, but even with nothing in it you won't see the colony clumping up if you swirl daily. At least that's always been my experience the times I used nothing to break up the myc. It may sound crazy but there's also a bit of a learning curve on how to swirl efficiently.
Jars with nothing in it, just plain swirling:

Clumping is not much of an issue if you're planning on pouring the LC, though.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Cultivation General Discussion [Re: Vinci]
#27333839 - 06/03/21 12:31 PM (2 years, 8 months ago) |
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Quote:
Vinci said: What y'all think about putting a blender lid on a fresh LC right after an agar drop and blending your wedge into the LC? Think it might speed the LC colonization?
It might take even longer because now the blended wedge will take longer to recover. Imo, it's best to let the wedge recover before you put it on a stir plate or even swirl. Once the wedge recovers, growth is going to be exponential in the liquid because lots of myc fragments are going to break up from the wedge when you swirl/stir.
If you want quick colonization, use no more than 150-200 ml and add 0,15% Agar by volumen [I like to mix and heat the lme(0,2-0,4%)and agar before pouring into the LC jars].
I don't use stirrers and my LC's colonize in a matter of 5-7 days from biopsy. I understand this may sound like BS but you can try it yourself and see. No stirrers used, a tube reaching the bottom for breaking up the colony, swirl once or twice a day once I see growth.
200 ml of LC is really a lot, you can noc a lot of shit with that and it colonizes way faster than a 500ml broth. You can always noc more LC's if you need more.
Edited by Josex (06/03/21 12:46 PM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,502
Loc: where?
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Re: Cultivation General Discussion [Re: Josex]
#27333976 - 06/03/21 03:10 PM (2 years, 8 months ago) |
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I always do 200-250ml broths. They are ready quicker and the volume is easier to handle and can inoculate a shit of grains.
Sometimes when noobs post entire quarts of lc its like, what exactly are you using all that power when they probably grow few tubs of cubes at most.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,825
Loc: Gaming the system
Last seen: 7 hours, 5 minutes
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Re: Cultivation General Discussion [Re: mushboy]
#27334011 - 06/03/21 03:50 PM (2 years, 8 months ago) |
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Yep I for sure underestimated how far lc goes at first. Even my little 1/2 pint jars half full of broth will make way more spawn than I need. Especially if you use the lc to make g2g masters.
--------------------
LAGM2020     
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Feasoghorm

Registered: 10/24/18
Posts: 4,384
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Re: Cultivation General Discussion [Re: A.k.a]
#27334043 - 06/03/21 04:26 PM (2 years, 8 months ago) |
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I swirled it about 3 days ago. I also dropped a fat round of a wedge in there when i inoculated it. Now im wondering how that's gonna get in my way haha
Yeah i read yall saying that it goes far so i only did a couple hundy full but 3 of them jst in case one or more is contam

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Vinci
I'm You



Registered: 08/26/19
Posts: 639
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Re: Cultivation General Discussion [Re: Feasoghorm]
#27334088 - 06/03/21 05:03 PM (2 years, 8 months ago) |
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I'mma cut the wedge in half and run it in 2 jars and blend one of them. Thinking the blend will also give the broth an oxygen boost.
-------------------- Vinci luh you
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
Last seen: 9 months, 7 days
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Re: Cultivation General Discussion [Re: Vinci]
#27334104 - 06/03/21 05:15 PM (2 years, 8 months ago) |
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If you all are looking for fast lc colonization. Squirt 1 cc of lc into a new lc jar. It will recover and colonize faster than starting again from another wedge.
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Vinci
I'm You



Registered: 08/26/19
Posts: 639
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Re: Cultivation General Discussion [Re: BrownBear]
#27334121 - 06/03/21 05:25 PM (2 years, 8 months ago) |
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Not expanding LC, inoculating.
-------------------- Vinci luh you
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Vinci
I'm You



Registered: 08/26/19
Posts: 639
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Re: Cultivation General Discussion [Re: Vinci]
#27334125 - 06/03/21 05:28 PM (2 years, 8 months ago) |
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I'm also side by side experimenting with making my slurries mostly out of canola oil with like 30% water. Testing starbones' canola theories. Too early to see any difference right now.
-------------------- Vinci luh you
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