Home | Community | Message Board


HighDesertSpores.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals CBD Edibles   PhytoExtractum Kratom Powder for Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   Amazon Agar, Parafilm, Petri Dish

Jump to first unread post. Pages: 1
OfflineConcentratedMind
Stranger
Registered: 05/22/21
Posts: 11
Last seen: 10 days, 20 hours
Finished preparing AGAR plates and innoc'd all now questions
    #27331425 - 06/01/21 03:05 PM (11 days, 6 hours ago)

Using C10's and Agar Tek I created 30 petris last night and after cooling took 13 syringes I had and did around 2 petris each of said strain of the 13 syringes. Now what? lol

After parafilming, labeling and putting each petri in a ziploc now what? I left them in my SAB with the lights off. What is good for short storage while petri is growing out? I know longterm they can go in fridge? But what about shortterm?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisiblearpeggiator
A Friend
Male


Registered: 12/15/13
Posts: 194
Loc: Hyperspace
Re: Finished preparing AGAR plates and innoc'd all now questions [Re: ConcentratedMind]
    #27331434 - 06/01/21 03:16 PM (11 days, 6 hours ago)

They can sit at room temp in the ziplock. If they are inoculated and wrapped, you can take them out of the ziplock bag. From what I've read you only need to store unwrapped, uninoculated plates in a sealed bag.

Also you're gonna need to make more plates if you used all of them for 13 of your syringes. You going to need to make a couple of agar transfers past your germination plate before putting them to grain.


--------------------


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleLadysKnight
Hello Ladies
 User Gallery

Registered: 10/09/15
Posts: 930
Re: Finished preparing AGAR plates and innoc'd all now questions [Re: arpeggiator]
    #27331443 - 06/01/21 03:26 PM (11 days, 6 hours ago)

You can use ziplocks instead of wrapping inoculated plates.
They store just fine at room temp. Ideally transfer from them before they takeover the whole plate. But with new germinating plates, you want to transfer as soon as you see clean mycelium.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineGuerrilla
Entangled


Registered: 01/30/21
Posts: 227
Loc: United Kingdom Flag
Last seen: 11 days, 6 hours
Re: Finished preparing AGAR plates and innoc'd all now questions [Re: LadysKnight]
    #27331474 - 06/01/21 03:53 PM (11 days, 6 hours ago)

Expect some funky looking growth and keep trying until you get the hang of it. Spore syringes aren’t clean and as a beginner you may likely need to perfect your sterile technique, so you will likely get a mixture of bacteria and mycelium, the idea is to transfer the healthy mycelium to new dishes to clean it up. If you get green mould in a plate it’s best to throw it away or if you must, take transfers from it last of all as the mould spores will be in the air while you’re working once you open the plate. It’s better to just start again


--------------------


Please help me find these prints:
YETI / GHOST / TAT / JACK FROST / JEDI MIND FUCK / LEUCISTIC / ALBINO STRAINS / COLOMBIAN RUST / TEXAS ORANGE COCK / STABLE CROSSES


Edited by Guerrilla (06/01/21 03:55 PM)


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineConcentratedMind
Stranger
Registered: 05/22/21
Posts: 11
Last seen: 10 days, 20 hours
Re: Finished preparing AGAR plates and innoc'd all now questions [Re: Guerrilla]
    #27331649 - 06/01/21 06:53 PM (11 days, 3 hours ago)

Thank you for the reply. Yes intend to make alot more plates for the syringes as I only used a few drops each plate. I have all the syringes cause I had planned to inject straight to grain but as I learned more here that was not key. So then I dove straight into agar and here I am.

All the plates are currently innoc'd and wrapped with parafilm so they can be kept out now?

Then plates that were poured and cool stay in ziplocs till used to be sterile?  Correct?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineAshtray161S
SettledNomad
Other


Registered: 03/21/21
Posts: 1,464
Loc: Rugby, England
Last seen: 34 minutes, 13 seconds
Re: Finished preparing AGAR plates and innoc'd all now questions [Re: ConcentratedMind]
    #27331789 - 06/01/21 09:06 PM (11 days, 56 minutes ago)

Just so u knw one drop is more than  enou, more than that can lead to too much liquid in ur plates. What I do is after cooling my needle I let a drop form on the end and streak it in a Z pattern on the plate.

Yep wait for them to grow, do a couple of transfers to be sure its clean, put to grain or LC, grow mushrooms.

To be sanitary, and keep the inside of the plates sterile, yes. I store my unused poured plates unwrapped in my SAB personally but I've heard in zip locks is fine.


--------------------
I AM PRETTY NEW. ALWAYS FOLLOW A TRUSTED CULTIVATOR OR A PROPER TEK
Does it pee when you burn?

FELLOW NEWBS, START HERE: https://www.shroomery.org/forums/showflat.php/Number/24420178
We need to stop abusing drug users. CHICKENS EDDY!
ACABEvenYourDad Anything Helps :begger::begger::begger: Spanging for prints!


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisiblec10h12n2o
becoming
 User Gallery


Registered: 01/21/15
Posts: 2,989
Loc: the abyss
Re: Finished preparing AGAR plates and innoc'd all now questions [Re: Ashtray161]
    #27340937 - 06/09/21 01:47 AM (3 days, 20 hours ago)

howd it go? you from syringes you should be transferring colonies from the later streaked zones asap


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineAspiringmycologist
Stranger
 User Gallery
Registered: 05/27/21
Posts: 49
Last seen: 6 hours, 14 minutes
Re: Finished preparing AGAR plates and innoc'd all now questions [Re: c10h12n2o]
    #27341474 - 06/09/21 01:03 PM (3 days, 8 hours ago)

Would one of the more experienced heads tell me what’s a good time frame to know if my agar is sterile? I made some plates about a week ago. Knocked up 4, made one control, and the rest are stored. The control has growth but the rest still look clean


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisiblec10h12n2o
becoming
 User Gallery


Registered: 01/21/15
Posts: 2,989
Loc: the abyss
Re: Finished preparing AGAR plates and innoc'd all now questions [Re: Aspiringmycologist]
    #27343710 - 06/11/21 12:20 AM (1 day, 21 hours ago)

The control has growth? If so its not sterile

By control you mean a plate you made that was not inoculated, right?


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineAspiringmycologist
Stranger
 User Gallery
Registered: 05/27/21
Posts: 49
Last seen: 6 hours, 14 minutes
Re: Finished preparing AGAR plates and innoc'd all now questions [Re: c10h12n2o]
    #27344412 - 06/11/21 02:41 PM (1 day, 7 hours ago)

Hey! Sorry  by control I meant one that I left out open just to make sure my agar wasn’t a dud. I made 16 and so far the sealed ones haven’t shown any growth yet. I knocked up 4 with 2 different mss syringes and neither has shown any growth yet either unfortunately.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Bridgetown Botanicals CBD Edibles   PhytoExtractum Kratom Powder for Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   Amazon Agar, Parafilm, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Whats with my agar plates? Frankenstein 5,461 6 04/08/02 05:13 PM
by Seuss
* Non sterilized agar plate bag, is it really not sterilized? EnDelfisE 102 5 06/09/21 07:36 AM
by DERRAYLD
* Re: mold in agar plate Anonymous 7,032 3 12/04/99 02:35 AM
by Anonymous
* Vert on Agar plates? Contam? Should I toss these tubs? manlord 105 4 06/09/21 04:56 PM
by manlord
* How many mycelium syringes from one agar plate? BBShrizz 2,887 3 05/10/01 04:10 AM
by ISaToriBluEI
* Agar help Nickthetrip 1,019 6 08/29/02 09:27 AM
by TripsAreForKids
* Re: agar plate question Psilowarrior 1,865 2 12/10/99 04:09 PM
by rebelmoon
* Agar....Re-using question Erbaliza 1,060 8 12/02/02 08:26 AM
by rhizo

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
183 topic views. 16 members, 102 guests and 60 web crawlers are browsing this forum.
[ Print Topic | ]
Search this thread:
Reliable Spores
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.005 seconds on 13 queries.