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Offlinekidcharlemagne
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Question about salvaging these stalled jars
    #27329927 - 05/31/21 05:12 AM (2 years, 10 months ago)



I inoculated 6 of a 10 jars from a pc cycle with a melmac LC.  The melmac was tearing through the spawn until I shook it two weeks ago after which they basically stalled.  That photo is after a month of colonizing and two weeks post shake.  On the right you’ll see the a different grain jar is almost fully colonized.  They don’t appear particularly bacterial to me but what do I know.  I’d really like to salvage these jars if possible.  I know for a lot if you it’s no big deal to start over but for reasons I’d like to salvage.  Should I let them continue to try and colonize, spawn as is, or can I attempt  some sort of peroxide/bleach dilution/gentamicin sulphate intervention? Thanks.

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Offlinetonz0Funguy
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Re: Question about salvaging these stalled jars [Re: kidcharlemagne]
    #27329960 - 05/31/21 06:01 AM (2 years, 10 months ago)

Smell test it!! If smells bad bury it outside may still get some fruits, don’t break it up and cover with 50/50 mix, the jar to the right looks good, might just be the culture, the only time you should worry about opening a jar is if there’s powdery mold spores. Case the top and try to fruit works also if u use Chems it’ll kill the myc, gentamicin might be ok I’ve never used it personally. Bad jars are hit and miss


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InvisiblebodhisattaMDiscordReddit
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Re: Question about salvaging these stalled jars [Re: kidcharlemagne]
    #27329964 - 05/31/21 06:04 AM (2 years, 10 months ago)

That jar is fucked. There's no saving it

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Offlinekidcharlemagne
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Re: Question about salvaging these stalled jars [Re: bodhisatta]
    #27329990 - 05/31/21 06:44 AM (2 years, 10 months ago)

Quote:

bodhisatta said:
That jar is fucked. There's no saving it




Hey Bod, what do you see in that pic that makes you say that? Trying to learn to see these things for myself.  So assuming the spawn was good and the LC bad, is the reason the mycelium tore through the spawn so well initially is that it was trying to outrun the bacteria and once I shook it, the bacteria was everywhere?  Out of curiosity, why wouldn’t a 1:10 bleach soak or a peroxide or gentamicin bath help?  Also, is it worth spawning to bulk to see what happens?  Thanks alot for help.

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Offlinekidcharlemagne
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Re: Question about salvaging these stalled jars [Re: bodhisatta]
    #27331335 - 06/01/21 11:24 AM (2 years, 10 months ago)

Would anyone else mind chiming in on what looks bad about these jars since bod is MIA?  I put pictures of the other jars below.  They aren’t slimy and they don’t smell.  Is there something in the way they look that would lead someone to think they are bacterial?  Remember they were shaken (2 weeks ago) I was just wondering if there’s a visual indicator of bacteria.  Thanks.



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InvisiblebodhisattaMDiscordReddit
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Re: Question about salvaging these stalled jars [Re: kidcharlemagne]
    #27331505 - 06/01/21 02:25 PM (2 years, 10 months ago)

What about them looks right? They don't look anything like healthy jars. They're barely colonizing. if they don't look healthy they're bad

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OfflineHindsight
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Re: Question about salvaging these stalled jars [Re: bodhisatta]
    #27331550 - 06/01/21 03:14 PM (2 years, 10 months ago)

Yes, specifically, those do not have even coverage over all parts of the grain. A healthy jar recovers 100% from a shake within 2-3 days. All grains should have an even white cover of mycelium. Yours show many grains with solid white coverage and many grains without any coverage at all. Looks to me like you had some sections of the jar that had bacterially infected grains, then you shook it which distributed those infected grains evenly across the jar, and now you have what you have.

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Invisiblebakedbeings
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Re: Question about salvaging these stalled jars [Re: Hindsight] * 1
    #27331568 - 06/01/21 03:26 PM (2 years, 10 months ago)

you dont "see" bacteria. the evidence of its presence is that the mycelium isnt recovering from the shake

toss em and start over

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Offlinekidcharlemagne
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Re: Question about salvaging these stalled jars [Re: bodhisatta]
    #27331665 - 06/01/21 05:15 PM (2 years, 10 months ago)


I just wanted to make sure you all were basing it on the fact that the jars hadn’t recovered from shaking rather than some visual cue I didn’t see.  Is there any reasonable chance if I spawn these to a shoebox they will colonize the substrate and push out a few fruits?  I was also wondering if there’s a chance the grain dried out, which happened to me before and when I dunked the grains, they took off.  Are pathogens and lack of h2o the only things that will stall a jar post shake?

Edited by kidcharlemagne (06/01/21 05:19 PM)

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Offlinekidcharlemagne
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Re: Question about salvaging these stalled jars [Re: bakedbeings]
    #27331673 - 06/01/21 05:22 PM (2 years, 10 months ago)

Quote:

Bakedbeings said:
you dont "see" bacteria. the evidence of its presence is that the mycelium isnt recovering from the shake

toss em and start over




There are a lot of visual cues of bacterial infection other than the absence of colonization that have been pointed out in the past in contamination threads.  I’m trying to make sure I’m not missing something.

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Re: Question about salvaging these stalled jars [Re: kidcharlemagne]
    #27331684 - 06/01/21 05:31 PM (2 years, 10 months ago)

I don't understand what you're missing. The jars just look fucked. Compare them to healthy jars. They don't have to be slimy or rainbow colored. The mycelium is obviously struggling. Probably won't get a grow if you spawn them. Try it and learn :shrug:

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OfflineHindsight
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Re: Question about salvaging these stalled jars [Re: bodhisatta]
    #27331706 - 06/01/21 05:47 PM (2 years, 10 months ago)

Plenty of moisture in those jars so the grains didn't dry out.

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Re: Question about salvaging these stalled jars [Re: Hindsight]
    #27331735 - 06/01/21 06:11 PM (2 years, 10 months ago)

If you insist on trying them then just shoebox them at a high grain ratio. I've pulled mush from grain like that but it's a bogus thing to make a habit of.


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Edited by Smartattack (06/01/21 06:12 PM)

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InvisibleAshtray161
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Re: Question about salvaging these stalled jars [Re: Smartattack]
    #27331762 - 06/01/21 06:44 PM (2 years, 10 months ago)

Quote:

Smartattack said:
If you insist on trying them then just shoebox them at a high grain ratio. I've pulled mush from grain like that but it's a bogus thing to make a habit of.



:whathesaid:
If u dont have much going on I dont see a problem trying it. I've gotten fruits from jars like that too but like what's been said, it's not good practice


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OfflineThreads2theSoul
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Re: Question about salvaging these stalled jars [Re: Ashtray161]
    #27332468 - 06/02/21 10:19 AM (2 years, 10 months ago)

I found this post helpful in identifying good clean spawn.

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