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Offlinemoshe1

Registered: 08/29/15
Posts: 259
Last seen: 4 hours, 24 minutes
Re: Help me understand my agar [Re: jomanda1990]
    #27327189 - 05/28/21 10:12 PM (17 days, 4 hours ago)

my main concern is identifying whats going on when i look at it! having problems is one thing not being able to identify them is a lot worse lmao

but i would take weak growth over mold any day- cause apparently all my trays have contam by the feedback im getting


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Edited by moshe1 (06/02/21 05:26 AM)


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 3,884
Re: Help me understand my agar [Re: moshe1]
    #27327239 - 05/28/21 11:07 PM (17 days, 3 hours ago)

Yeah dude make some plates with more nuteage and transfer to those plates from the crazy looking ones to see if it comes out nice.


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OfflineCrackatoaS
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Re: Help me understand my agar [Re: Feasoghorm] * 1
    #27327280 - 05/28/21 11:58 PM (17 days, 2 hours ago)

My recipe is 10g agar, 7-8g LME per 600ml plus nutritional yeast. The yeast has really helped.


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Offlinemoshe1

Registered: 08/29/15
Posts: 259
Last seen: 4 hours, 24 minutes
Re: Help me understand my agar [Re: Crackatoa]
    #27327330 - 05/29/21 12:54 AM (17 days, 1 hour ago)

thanks crackatoa, ill try adding some yeast and see what it does. Congrats on your TC btw

would you say that its still possible that i'm recipe/nutritional issues only?  or you are also pretty made up as well that my plates all having a contam issue going on


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Edited by moshe1 (05/29/21 12:55 AM)


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 3,884
Re: Help me understand my agar [Re: moshe1]
    #27327334 - 05/29/21 01:04 AM (17 days, 1 hour ago)

I understand that you did not ask me, but at this point im basically convinced that what we are seeing is a nute issue. I feel bad that i may have put the contamination theory in your head. I shud knw this shit by now, but i was basically retarded till the beginning of this year. So you'll have to excuse me.


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Offlinemoshe1

Registered: 08/29/15
Posts: 259
Last seen: 4 hours, 24 minutes
Re: Help me understand my agar [Re: Feasoghorm]
    #27327352 - 05/29/21 01:33 AM (17 days, 1 hour ago)

no problems my dude, and i appreciate hearing that from anyone actually, so i'll take it. Seriously, go search for an answer to an issue like im having with the search function you ll end up ripping your hair out.

you will go from being convinced its contam, to convinced its nutrients and back and forth with every other thread you read into lol, i'm definitely reporting back step by step to put a nail in this coffin


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Edited by moshe1 (05/29/21 01:34 AM)


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OfflineBlackrainbow2
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Re: Help me understand my agar [Re: moshe1]
    #27327372 - 05/29/21 01:49 AM (17 days, 1 hour ago)

another option is get your self a few plates from a vendor and see if they preform normal and that may get you answers, on if it's your agar or something else.. I think 10 plats are like $22..


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OfflineCrackatoaS
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Re: Help me understand my agar [Re: Blackrainbow2]
    #27327686 - 05/29/21 10:34 AM (16 days, 16 hours ago)

I believe it may be both, I feel like there's still some contamination in there hiding they can cleaned and some have really good looking edges to snag but I also feel once we figure this nute issue out the good myc will be easy to see and snag.


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Offlinemoshe1

Registered: 08/29/15
Posts: 259
Last seen: 4 hours, 24 minutes
Re: Help me understand my agar [Re: Crackatoa]
    #27328174 - 05/29/21 06:27 PM (16 days, 8 hours ago)

bumpdate :

fresh melmak spores went to agar just over a week ago. went a day too long but gonna transfer out of these today. showing some similarities to previous plates though- same agar recipe.  i'll be transferring out of the odd looking plates from the original post next couple days with a higher nutritional agar and lower to see the difference




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Edited by moshe1 (05/29/21 07:38 PM)


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OfflineCrackatoaS
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Re: Help me understand my agar [Re: moshe1]
    #27328193 - 05/29/21 06:47 PM (16 days, 8 hours ago)

:thisisgonnabegood:


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Offlineverum subsequentis
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Registered: 03/22/16
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Re: Help me understand my agar [Re: Crackatoa]
    #27328239 - 05/29/21 07:33 PM (16 days, 7 hours ago)

Looking good. Looks like there's goodies in all those plates.


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Offlinemoshe1

Registered: 08/29/15
Posts: 259
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Re: Help me understand my agar [Re: verum subsequentis]
    #27328242 - 05/29/21 07:37 PM (16 days, 7 hours ago)

room still scrubbing- im thinking i should do the transfers in my SAB this time around? or should i continue working in front of flow?  haven't used the good ol' glove box in so long


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Offlineverum subsequentis
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Re: Help me understand my agar [Re: moshe1] * 1
    #27328245 - 05/29/21 07:39 PM (16 days, 7 hours ago)

Why wouldn't you use the flow? I would.


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Offlinemoshe1

Registered: 08/29/15
Posts: 259
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Re: Help me understand my agar [Re: verum subsequentis]
    #27328250 - 05/29/21 07:43 PM (16 days, 7 hours ago)

idk, i was under the impression transferring from contam plates infront of the flowhood = bad

i'd much much rather keep working in front of the hood lol spent a lot of time building the big boy


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Offlineverum subsequentis
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Re: Help me understand my agar [Re: moshe1] * 1
    #27328254 - 05/29/21 07:45 PM (16 days, 7 hours ago)

It's only bad if you are opening a plate with nasties that are sporulating. Even then, I've pulled it off in the flow several times.


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OfflineConcreteWaves
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Re: Help me understand my agar [Re: verum subsequentis]
    #27328272 - 05/29/21 08:00 PM (16 days, 6 hours ago)

Sector n xfer only your strongest growth. Sacrifice the rest; not ballin. Shooting for rhizmophoric action. If you went from spores id xfer 2 or 3 times before noccing up spawn at the least.


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Offlineverum subsequentis
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Re: Help me understand my agar [Re: ConcreteWaves]
    #27328362 - 05/29/21 09:16 PM (16 days, 5 hours ago)

Shooting for rhizo seems to be somewhat of a fool's errand. Shoot for clean plates. I believe I'd have perfectly fine transfers on the next round if those were mine.


Edited by verum subsequentis (05/29/21 09:16 PM)


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 3,884
Re: Help me understand my agar [Re: verum subsequentis] * 1
    #27328445 - 05/29/21 10:24 PM (16 days, 4 hours ago)

Them are some pro germ plates :thumbup:

Cant wait to see your findings about the plate nutes. Your doing the Lord's work, moshe1


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Offlinemoshe1

Registered: 08/29/15
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Re: Help me understand my agar [Re: Feasoghorm]
    #27328472 - 05/29/21 10:45 PM (16 days, 4 hours ago)

Quote:

verum subsequentis said:
Shooting for rhizo seems to be somewhat of a fool's errand. Shoot for clean plates. I believe I'd have perfectly fine transfers on the next round if those were mine.




hoping the first transfers come out clean as well, lets see what happens. just finished up with another set of 20 plates, most of em were used on those new germs, and a few were used on those ugly t1 and t2s from first post.


Quote:

Feasoghorm said:
Them are some pro germ plates :thumbup:

Cant wait to see your findings about the plate nutes. Your doing the Lord's work, moshe1




thanks! same here man, hoping to save time for all the future people on this. my germ plates looked pretty decent on my first sets as well before they went wild on me, fingers crossed.


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Offlinemoshe1

Registered: 08/29/15
Posts: 259
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Re: Help me understand my agar [Re: moshe1]
    #27330703 - 05/31/21 09:30 PM (14 days, 5 hours ago)

BUMPDATE  5/31

Here are those fresh melmaks on their first transfer today. These are also 10g agar to 7-8g LME to just over 400 ML water.

Prepped two new agar bottles, one with ~5G LME, 10G Agar, 400ML and the other with the ~7G LME + 1G Nutritional Yeast, 400ML.  Will transfer to those next coming days.

Just got back from the supermarket and they had very fine stoneground BRF on clearance half off, snagged 5 pounds for only like 10 bucks- so at some point BRF agar will be on trial






transparent layer of myc growing outwards only visible well under light hard to take pictures.


Edited by moshe1 (06/01/21 03:33 AM)


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