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OfflineTe0nanacatl
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First ever spawning to sub, any advice would be helpful
    #27316513 - 05/20/21 04:44 PM (28 days, 20 hours ago)

I recently came into possession of a few quart jars of inoculated grain, and I will be attempting to spawn them this weekend. I have no experience with mushroom cultivation, until two weeks ago, but for the last two weeks I’ve been trying to read up as much as I can, so I have any shot at being successful.

These are the jars that I have, and I’m aware they look bad.



From what I know, they’ve been fully colonized for at least 3 months now, and a lot of them fell to various contaminations, these are the only ones that have “survived”. From what I’ve found, the suggested way to handle this situation is to do a G2G transfer, but I don’t have any PC at the moment, so I would like to try to spawn them, and see what happens. Also I have 5 strains in 6 jars, so I don’t have 6qt for a single monotub.

The plan is following:

I’m making CVG substrate in a cooler, letting it pasteurize overnight. I’m spawning to substrate in the morning, but first breaking up the cakes and dunking them for ~10 minutes using bottled water, to reduce the chance of contamination. The bathroom where I will be spawning in will be cleaned by 10% bleach, followed by 70% iso alcohol. Liners are already cut, will be sanitized also, and I’m putting everything in a 5qt, unmodified shoebox monotub. Ratio will be 1 to 1, again to give maximum chance to mycelium to develop in the sub.
Once all the tubs are filled with 2.5 inches of sub and spawn, I plan to put them in garbage bags, to limit FAE and light.

Questions are:

Will dunking the spawn help, or am I just risking already weak myc for no gain?

Is keeping them in garbage bags helping? The way I see it, I have to go with a shoebox since I have to go with 1/1 ratio to have any chance of this myc growing, but then I’m in danger of drying out because of a small tub + only 2.5 inches of sub. My hope is that the garbage bag will help with that. Should I sterilize the garbage bag if closed monotub is stored in it?

-----------

The last question is completely unrelated to this whole story, and it’s related to SABs. I also inherited a syringe with some LC in it (also some spore prints), and I would like to start working with agar. It seems more controlled to me than just inoculating jars immediately. The question is, is it possible instead of cutting out holes for hands, just to cut out the bottom of one of the sides, like so.



I did find similar images, something like this



And also a guy claiming that he took out an entire side off, but no definite answer, would this design be a bad idea?


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OfflineAshtray161S
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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27316521 - 05/20/21 04:53 PM (28 days, 20 hours ago)

Quote:

Te0nanacatl said:
I recently came into possession of a few quart jars of inoculated grain, and I will be attempting to spawn them this weekend. I have no experience with mushroom cultivation, until two weeks ago, but for the last two weeks I’ve been trying to read up as much as I can, so I have any shot at being successful.

These are the jars that I have, and I’m aware they look bad.



From what I know, they’ve been fully colonized for at least 3 months now, and a lot of them fell to various contaminations, these are the only ones that have “survived”. From what I’ve found, the suggested way to handle this situation is to do a G2G transfer, but I don’t have any PC at the moment, so I would like to try to spawn them, and see what happens. Also I have 5 strains in 6 jars, so I don’t have 6qt for a single monotub.

The plan is following:

I’m making CVG substrate in a cooler, letting it pasteurize overnight. I’m spawning to substrate in the morning, but first breaking up the cakes and dunking them for ~10 minutes using bottled water, to reduce the chance of contamination. The bathroom where I will be spawning in will be cleaned by 10% bleach, followed by 70% iso alcohol. Liners are already cut, will be sanitized also, and I’m putting everything in a 5qt, unmodified shoebox monotub. Ratio will be 1 to 1, again to give maximum chance to mycelium to develop in the sub.
Once all the tubs are filled with 2.5 inches of sub and spawn, I plan to put them in garbage bags, to limit FAE and light.

Questions are:

Will dunking the spawn help, or am I just risking already weak myc for no gain?

Is keeping them in garbage bags helping? The way I see it, I have to go with a shoebox since I have to go with 1/1 ratio to have any chance of this myc growing, but then I’m in danger of drying out because of a small tub + only 2.5 inches of sub. My hope is that the garbage bag will help with that. Should I sterilize the garbage bag if closed monotub is stored in it?

-----------

The last question is completely unrelated to this whole story, and it’s related to SABs. I also inherited a syringe with some LC in it (also some spore prints), and I would like to start working with agar. It seems more controlled to me than just inoculating jars immediately. The question is, is it possible instead of cutting out holes for hands, just to cut out the bottom of one of the sides, like so.



I did find similar images, something like this



And also a guy claiming that he took out an entire side off, but no definite answer, would this design be a bad idea?




Dude I hate to tell u this but i dont think a single one of those jars has mush mycelium in them. And if by some miracle a couple do they are not alone. I wouldnt spawn those... Its time to toss and restart mate :/
Sorry 4 ur loss


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OfflinePBJ710
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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27316618 - 05/20/21 05:57 PM (28 days, 19 hours ago)

Jar 4 only looks pretty bad - the rest are complete trash - I wouldn't waste any time on them personally.

You need to start reading up on how to start spores on agar and once you have a clean culture growing you can start expanding it.  You WILL need a PC of some sort to expand it to grains.

The purpose of a SAB is to provide a place with almost NO air movement internally.  Giant openings (especially at the bottom where contaminents fall to) are generally frowned upon.  There's plenty of teks on how to easily make one that works well - why try to reinvent the wheel?


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OfflineBakedbeings
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Re: First ever spawning to sub, any advice would be helpful [Re: PBJ710]
    #27316777 - 05/20/21 08:03 PM (28 days, 17 hours ago)

to repeat the obvious, those jars are fucked
:pink:

moving on...assuming that LC is good (where is it from? how old is it?) you could inoc some new grain with it, but your safest bet is getting that spore print onto some agar. read this:

https://www.shroomery.org/forums/showflat.php/Number/20111637

about the SAB, two little arm holes is just the best way to do it. deviating will only mess you up. before i figured out how to cut holes i tried just working with the tub on its side and it was a disaster

https://www.shroomery.org/forums/showflat.php/Number/23990913


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OfflineCocaineBuffet
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Re: First ever spawning to sub, any advice would be helpful [Re: Bakedbeings]
    #27316853 - 05/20/21 09:22 PM (28 days, 15 hours ago)

Quote:

Te0nanacatl said:
I recently came into possession of a few quart jars of inoculated grain




You should slap whoever gave you those fucked up jars.


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OfflineAshtray161S
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Re: First ever spawning to sub, any advice would be helpful [Re: CocaineBuffet]
    #27316887 - 05/20/21 10:14 PM (28 days, 14 hours ago)

Quote:

CocaineBuffet said:
Quote:

Te0nanacatl said:
I recently came into possession of a few quart jars of inoculated grain




You should slap whoever gave you those fucked up jars.



:whathesaid: :abduction:


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OfflineTe0nanacatl
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Re: First ever spawning to sub, any advice would be helpful [Re: Ashtray161]
    #27316975 - 05/21/21 12:28 AM (28 days, 12 hours ago)

I'm at peace with the fact that this spawning could completely fail, but I can't see what is the difference between throwing out the jars now, and spawning them, and throwing out the sub if it looks bad?
I would've wasted half a brick of coco coir, and a few cups of verm, but on the other hand I also get to practice making the sub and my sanitation technique.

Am I missing some obvious downside?

I understand they look bad, but most of them don't have obvious black/green/brown spots, and I'm hoping the yellow stuff is mostly myc discharge. But if some of them also smell bad, I probably won't even bother with spawning them. The reason I'm so hung up on attempting to do something with the jars is because they contain rare strains that I don't see me getting any other way. Also, if there is no potential problem other than wasted time and effort, I would still like to try. I can't get my hands on a PC for at least a week, so I wouldn't be doing anything else :laugh:

Quote:

Bakedbeings said:
about the SAB, two little arm holes is just the best way to do it. deviating will only mess you up. before i figured out how to cut holes i tried just working with the tub on its side and it was a disaster

https://www.shroomery.org/forums/showflat.php/Number/23990913




I've read all of Bod's tecks, I was just asking about the holes because I have big hands, so I would probably have to go bigger than even 6 inch, and at that point what is really the difference from cutting the bottom out? :laugh:

But yes, I will go with holes, I'll just get a few pieces of cardboard and test different sizes of holes.


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OfflinePBJ710
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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27317004 - 05/21/21 01:45 AM (28 days, 11 hours ago)

Sorry to be the one to tell you this but they all have significant contamination going on. 

While you're certainly welcome to do it for the sake of science, I doubt you will learn anything other than how fucked up those jars were.

If you're having difficulty with the holes, you can heat the edge of a coffee can (or other simlar sized can) and melt through the plastic to create a nice round hole thats plenty big enough for my #8 1/4 gloves to easily go though :wink:

Don't get too hung up on genetic variety names unless there was something special about the specific culture.  I would bet most if not all could be found in the marketplace within a couple months...


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27317005 - 05/21/21 01:46 AM (28 days, 11 hours ago)

That`s for sure a bacterial mess, but your project honors you :thumbup:

Before you carry them to shoeboxes, i`d suggest the Josex' Poke: No Mercy for Bacteria.

To me the best method to cut down bacteria if you want to save your rare strains.
I had some heavy dirty syringes always performing well on brf pucks.
But we`re just talking bacteria, this wouldn`t work with mold.
It´s not much time you lose and i bet it will work.

Cutting the bottom out?
You want it standing upside down to have a flat surface.
If you have bigger hands, try to melt holes with an empty 5 liter beer barrel.
I did mine, they`re a hair over 6".
Empty it into your throat in one go, and start work :biggrin:

In the end i wanna say, i once had a jar that looked even worse than yours,
i spent to a shoebox and it produced one decent flush.
Bacteria isn`t the end of the road.

              :cookiemonster:


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OfflineTe0nanacatl
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Re: First ever spawning to sub, any advice would be helpful [Re: Goatrider]
    #27317049 - 05/21/21 03:55 AM (28 days, 9 hours ago)

Quote:

PBJ710 said:
Don't get too hung up on genetic variety names unless there was something special about the specific culture.  I would bet most if not all could be found in the marketplace within a couple months...




I'm sure they can be found on the marketplace, but I'm not in USA or western EU, so I'm guessing my marketplace/trading options will be limited.

Quote:

Goatrider said:
Before you carry them to shoeboxes, i`d suggest the Josex' Poke: No Mercy for Bacteria.





This is sounds great, the only issue is I don't have PC yet, but I'll have it in a week or so. I will probably attempt to do it with the jars that really smell bad, I have nothing to lose :laugh:


Quote:

Goatrider said:
In the end i wanna say, i once had a jar that looked even worse than yours,
i spent to a shoebox and it produced one decent flush.
Bacteria isn`t the end of the road.

              :cookiemonster:




That's the dream scenario, I would be beyond happy if only one of these gave me some fruits 

:manson:


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OfflineAshtray161S
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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27317724 - 05/21/21 05:54 PM (27 days, 19 hours ago)

I mean, do you my friend, honestly thou I just dont see much purpose in trying to spawn those. I would put real money down that theyre not gonna fruit.
Youll also be (and I dont think this is a huge deal like some people do but still) spreading a ton of endospores (from the bacteria) and fungal spores (from the mold) all over your grow/fruiting workspace.
Those are pretty far gone man. Again, I dont even know if there is any healthy cubie mycelium in those jars. I also dont make this as huge of a deal as others but there are legit health concerns from exposure to certain molds and (as im sure youre aware) bacteria. Botulism isnt something to fuck around with.

If i were you, id scrap those jars, clean em well, refill em, re knock em, start again.
I think ur just gonna waste time if u try and "save" those jars.
:2cents:


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InvisibleMycelium Juice
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Re: First ever spawning to sub, any advice would be helpful [Re: Ashtray161] * 1
    #27317912 - 05/21/21 09:07 PM (27 days, 15 hours ago)

Look at that sweet, sexy, THIC mycelium juice  :inlove3:

:weirdout:


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Re: First ever spawning to sub, any advice would be helpful [Re: Mycelium Juice]
    #27317928 - 05/21/21 09:17 PM (27 days, 15 hours ago)

I had some jars like that too. About a 2 year experiment.
Mine went to the trash, Ill try spawning anything, but not that.



You wont be happy about the smell that comes out when you open them.



:shiiiiit:

Also, the foil could have come off those about 7 years ago.


Edited by Smartattack (05/21/21 09:19 PM)


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InvisibleMycelium Juice
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Re: First ever spawning to sub, any advice would be helpful [Re: Smartattack]
    #27317935 - 05/21/21 09:25 PM (27 days, 15 hours ago)

:whacker:

Jesus Smart!  Anyone else???  What ya got?!  Friday night just got turned up a notch


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OfflineAshtray161S
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Re: First ever spawning to sub, any advice would be helpful [Re: Smartattack]
    #27317939 - 05/21/21 09:27 PM (27 days, 15 hours ago)

Quote:

Smartattack said:
I had some jars like that too. About a 2 year experiment.
Mine went to the trash, Ill try spawning anything, but not that.



You wont be happy about the smell that comes out when you open them.



:shiiiiit:

Also, the foil could have come off those about 7 years ago.




:sip:


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OfflineTe0nanacatl
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Re: First ever spawning to sub, any advice would be helpful [Re: Smartattack]
    #27318140 - 05/22/21 01:49 AM (27 days, 11 hours ago)

Quote:

Ashtray161 said:
I mean, do you my friend, honestly thou I just dont see much purpose in trying to spawn those. I would put real money down that theyre not gonna fruit.
Youll also be (and I dont think this is a huge deal like some people do but still) spreading a ton of endospores (from the bacteria) and fungal spores (from the mold) all over your grow/fruiting workspace.





I'm definitely keeping that in mind, the shoeboxes will be kept in a completely separated room, nowhere near where I plan on keeping future monotubs. Also, I plan on scrubbing everything with 20% bleach solution.

Quote:

Smartattack said:
You wont be happy about the smell that comes out when you open them.





If that's the case I will just toss it. I still haven't opened these jars at any point, so I will smell them tonight for the first time. But, considering your jars look better than mine and they got tossed, it doesn't look good :laugh:
Again, I'm not very hopeful that this will succeed, it's just that I don't have anything better to do until the PC gets here, and I don't see any major risks with attempting to birth them.

But if they don't pass the smell test, I will probably just toss them :whiteflag:


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InvisibleMycelium Juice
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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27318232 - 05/22/21 05:05 AM (27 days, 8 hours ago)

I could always take them off your hands...

:cujo:


Edited by Mycelium Juice (05/22/21 05:42 AM)


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27318453 - 05/22/21 10:00 AM (27 days, 3 hours ago)

be VERY careful about the 'smell test'. i only smell mycelium that looks healthy just to double check. if something is VISIBLY contaminated theres no need to risk brain damage by inhaling a bunch of mystery microbes. if im not wrong someone on this site ended up in a coma that way


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Re: First ever spawning to sub, any advice would be helpful [Re: Bakedbeings]
    #27318591 - 05/22/21 11:38 AM (27 days, 1 hour ago)

Quote:

Bakedbeings said:
someone on this site ended up in a coma that way





Wut?


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OfflineTe0nanacatl
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Re: First ever spawning to sub, any advice would be helpful [Re: Bakedbeings]
    #27318594 - 05/22/21 11:41 AM (27 days, 1 hour ago)

Quote:

Bakedbeings said:
be VERY careful about the 'smell test'. i only smell mycelium that looks healthy just to double check. if something is VISIBLY contaminated theres no need to risk brain damage by inhaling a bunch of mystery microbes. if im not wrong someone on this site ended up in a coma that way




I thought about that, I think the best approach would be like they taught us in chemistry regarding smelling potentially dangerous chemicals. Just cracking the jar and wafting the air with my hand towards my face.

I didn't plan on breathing in a lungful of some random spores/bacteria :laugh:


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Re: First ever spawning to sub, any advice would be helpful [Re: Bakedbeings]
    #27318601 - 05/22/21 11:47 AM (27 days, 1 hour ago)

Quote:

Bakedbeings said:
if im not wrong someone on this site ended up in a coma that way




Who knows?
Has it been proven?
I wouldn`t take a sniff from a fusarium jar though :thumbup:

                :cookiemonster:


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OfflineTe0nanacatl
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Re: First ever spawning to sub, any advice would be helpful [Re: Goatrider]
    #27319540 - 05/23/21 05:02 AM (26 days, 8 hours ago)

I opened the jars a few hours ago, 3 of them smelled like death as soon as I cracked them, so I'm just tossing them.
Out of the 3 that remained, 2 had almost no scent, just a very mild, earthy smell, and the last one (which is one of the jars with a lot of myc fluid) had a very antibiotic-y scent, but once I drained the fluid, it also had almost no scent, so I spawned it to sub as well.
The mycelium was very dense, there was no way I could break it up by just shaking the jar, I had to "cut" it in pieces with a spoon. I didn't notice any molds on the inside of the mycelium, but I'm aware that doesn't mean anything.

Anyway I have three shoeboxes now, I've put them in separate garbage bags and I plan on checking them in 10 days to see if anything is happening.


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27319550 - 05/23/21 05:23 AM (26 days, 7 hours ago)

You did well tossing them death jars :thumbup:

Keep on posting mate!

            :cookiemonster:


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Re: First ever spawning to sub, any advice would be helpful [Re: Bakedbeings]
    #27319557 - 05/23/21 05:38 AM (26 days, 7 hours ago)

Quote:

Bakedbeings said:
be VERY careful about the 'smell test'. i only smell mycelium that looks healthy just to double check. if something is VISIBLY contaminated theres no need to risk brain damage by inhaling a bunch of mystery microbes. if im not wrong someone on this site ended up in a coma that way




no way lol is this forreal


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Re: First ever spawning to sub, any advice would be helpful [Re: Goatrider]
    #27319654 - 05/23/21 09:23 AM (26 days, 3 hours ago)

no clue, i remember reading about it lurking on here a few years ago. i doubt its provable with this whole community being anonymous. but i hate to let the truth get in the way of a good story :smile:


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Re: First ever spawning to sub, any advice would be helpful [Re: Bakedbeings]
    #27332401 - 06/02/21 11:45 AM (16 days, 1 hour ago)

Ten days have passed, and today I've checked my shoeboxes for the first time.

Looks like only one has a chance of surviving, but I guess you'll tell me what's growing in it.



Box number 1 has nothing growing in it except a tiny spot of mold. The second one looks promising to me, but I have no idea what the tiny white dots are. The third one smells bad besides looking bad :smile:

The plan is to throw out the boxes 1 and 3, a dub-tub the second one and wait to see what grows. I would also like to case the box, since I have some leftover verm. I don't plan on pasteurizing the verm, just mix it with a bit of bottled water and spread it over top. If any of this is a mistake, let me know, I will probably do this tomorrow morning.


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27332493 - 06/02/21 12:43 PM (16 days, 22 minutes ago)

Toss the two with cobweb mold, the white dots look like primordia however I think I see some cobweb there too? Hard to tell because of the photo quality. The fully colonized one looks great. Instead of casing with straight verm I’d use 50/50 peat & verm pasteurized. There are a number of teks on how to do that. If you decide to use straight verm just layer it on top about ~1/4” and heavily mist it. It will be easier to handle this way. You could also just let it fruit uncased. I’m seeing a lot of primordia forming so its already starting to fruit.


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Edited by PitcherCrab (06/02/21 12:44 PM)


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Re: First ever spawning to sub, any advice would be helpful [Re: PitcherCrab]
    #27333218 - 06/03/21 12:52 AM (15 days, 12 hours ago)

When you look at the sub in normal light, without flash, I think the white dots are grains of verm, either way, there is no sign of mycelium in boxes 1 and 3.

I tossed those two, and I'm fruiting the second one. I read up on straight verm casings, and RR says it's only a step above no casing at all, and since I can't get peat moss and lime until monday, I'll just go with no casing.
My thinking is, since I don't have the benefit of casing layer to keep the moisture in, I won't dubtub it for now, I'll just move the shoebox out of the taped up trash bag to improve FAE, mist it once to trigger pinning and leave it closed. It doesn't make sense to me to flip the lid, since the shoebox is much smaller than a monotub, I think it would dry out too quickly, and besides I'll get enough FAE through the imperfect lid seal. Again, if I'm wrong on anything please correct me.

I honestly can't believe even one box mad it to colonization, considering the state of those jars, if I get a single mushroom I'm happy :laugh:


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27333231 - 06/03/21 01:09 AM (15 days, 11 hours ago)

Based on how those primordia are looking you’ll for sure be getting mushrooms! I don’t think you need to mist it at all... looks to me like its happy exactly where its at. If you do mist just mist the air above the mycelium and let it float down on to the surface, misting directly and too much can cause pins to abort. Dub tubbing wouldn’t hurt either, you could offset the top tub a little to leave a crack to help give it more FAE to trigger pinning. Or alternatively put the whole thing into a larger mono. That’s my preferred way to fruit shoeboxes.

As for a casing layer, get all the supplies and do it next time with the clone you take from that fully colonized box. Make sure to post updates with how the fruiting goes!


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Re: First ever spawning to sub, any advice would be helpful [Re: PitcherCrab]
    #27333237 - 06/03/21 01:21 AM (15 days, 11 hours ago)

Yup, I just misted a bit above the sub, got tiny beads of water on mycelium, that should be fine.

The plan was originally to put them in a larger mono, I even bought one that could fit all three of them (I was optimistic :smile:), but I think it's too big to put just one inside. Considering Bod's shoebox tek, if you can just put a bag with holes on top during fruiting, I guess you can do pretty much anything and it will grow. The way I understand it, you just need enclosed space to keep in the moisture, but still allow FAE.


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27333241 - 06/03/21 01:41 AM (15 days, 11 hours ago)

Yup! You got it. Enclosed space with some way of providing FAE. Even myco bags work and their filter patches don’t really provide too much FAE.


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Re: First ever spawning to sub, any advice would be helpful [Re: PitcherCrab]
    #27333413 - 06/03/21 06:42 AM (15 days, 6 hours ago)

Nice, hopefully I get some pins soon. Thank you for your help PitcherCrab :bow2:


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27333421 - 06/03/21 06:54 AM (15 days, 6 hours ago)

those jars

:ohshi:


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Re: First ever spawning to sub, any advice would be helpful [Re: coversall]
    #27333547 - 06/03/21 09:41 AM (15 days, 3 hours ago)

Oh, I forgot the specify which jar made it through :laugh:



Out of all these, jar number 5 was the winner (bottom row, middle). 1,3 and 4 were tossed during spawning, while 2 and 6 (for which I thought had the most potential) only managed to grow cobwebs  :spiderrevenge:


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27333639 - 06/03/21 10:54 AM (15 days, 2 hours ago)

Wow. I’m surprised you got anything out of those!


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27333645 - 06/03/21 10:59 AM (15 days, 2 hours ago)

Quote:

Te0nanacatl said:
Nice, hopefully I get some pins soon. Thank you for your help PitcherCrab :bow2:




A good light really helps with pinning too. I just did some TOC and TWC and the TOC finished the first flush in 15 days from spawning and the TWC is juuuuuust starting now after over a month of being in fruiting. It all changed once I put an LED shoplight over them a couple days ago. Get something in the daylight spectrum (I use a 5600k bulb) and have it on a 12h on/12h off timer.


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Re: First ever spawning to sub, any advice would be helpful [Re: PitcherCrab]
    #27334525 - 06/04/21 01:23 AM (14 days, 11 hours ago)

I have some LED light lying around at ~6000k that I can put above the shoebox.
Is it good enough to have it shining through the lid, what do you think about something like this

https://www.amazon.com/Govee-Upgraded-Dimmable-Daylight-Adhesive/dp/B07XHLGSXN

I could put this on the underside of the lid, that way it's shining directly on the sub. Is it unnecessary?


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27334544 - 06/04/21 01:52 AM (14 days, 11 hours ago)

Shining through the lid is perfect. That’s exactly what I do!


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Re: First ever spawning to sub, any advice would be helpful [Re: PitcherCrab]
    #27335874 - 06/05/21 03:16 AM (13 days, 9 hours ago)

The pins have arrived!!  :mushroomgrow:  :mushroomgrow:  :mushroomgrow:



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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27335896 - 06/05/21 03:53 AM (13 days, 9 hours ago)

Nice dude. Just let them do their thing and you will have mushrooms in no time!


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Re: First ever spawning to sub, any advice would be helpful [Re: PitcherCrab]
    #27336073 - 06/05/21 10:11 AM (13 days, 2 hours ago)

wow dude congrats on making this work! im sure youll be making LOTS of spore prints from these miracle babies


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Re: First ever spawning to sub, any advice would be helpful [Re: Bakedbeings]
    #27336083 - 06/05/21 10:21 AM (13 days, 2 hours ago)

Holy FUCK!!!  You made it.  Nice work Te0. 
I think you've got the right attitude with experimenting and willingness to fail to be good at this hobby. 

Grade A work my friend.
Mad props to Pitcher Crab for helping light the way as well.

Quote:

Te0nanacatl said:
I have some LED light lying around at ~6000k that I can put above the shoebox.
Is it good enough to have it shining through the lid, what do you think about something like this

https://www.amazon.com/Govee-Upgraded-Dimmable-Daylight-Adhesive/dp/B07XHLGSXN

I could put this on the underside of the lid, that way it's shining directly on the sub. Is it unnecessary?





I use these exact lights rn.  Been going strong for over 8 months, and you can't beat that price point.


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Re: First ever spawning to sub, any advice would be helpful [Re: Mycelium Juice]
    #27336146 - 06/05/21 11:35 AM (13 days, 1 hour ago)

Thanks everyone, I'm just glad I'll get the spore prints from one of those jars, it's better than nothing. Like I said in the beginning, I have nothing to lose, even if they all failed I got some practice in making the sub and the whole process of spawning.

Anyway, now I've been reading about the lights, and as I see it, the brighter the better, and as close as possible to daylight, so > 6000k. My question is, people list more consistent pinning as the biggest advantage when using lights, so is the light still necessary once the pinning is done and mushrooms are growing?

Quote:

Mycelium Juice said:
I use these exact lights rn.  Been going strong for over 8 months, and you can't beat that price point.




Cool, do you bother with putting them under the lid, or do you just route them above the tub?


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27336218 - 06/05/21 01:05 PM (13 days, 16 seconds ago)

From what I've found on this site, 6500k seems to be the sweet spot.

Yes, keep the lights going while fruiting.  It will give the mushrooms a direction to grow, as well as help bring out their natural coloring.


Quote:

Te0nanacatl said:
Cool, do you bother with putting them under the lid, or do you just route them above the tub?





Putting them under the lid sounds like a pain in the ass to me! :lol:

I noob-ly threw the lights on top of the tubs to start so they would stay closer...
BUT quickly ended up securing them to the shelf above for ease of access and to keep it clean. (I use a 5 tiered rack/shelving system)
It was a true pain in the ass not having them secure fyi.
You can still droop them to stay close to tubs, but definitely secure them above and not in!
Basically set it, and now I get to forget it. :rockon::rocket::mushroom2:



Edited by Mycelium Juice (06/05/21 01:36 PM)


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Re: First ever spawning to sub, any advice would be helpful [Re: Mycelium Juice]
    #27338370 - 06/07/21 04:21 AM (11 days, 8 hours ago)

The pins are coming along nicely, the biggest one is a bit over an inch tall. I have a few questions regarding surface conditions. If I'm correct, you should mist once the surface looks dry, and there are no droplets on the mycelium. Here is a picture of the tub before I misted it

  And this is after

I'm not sure how visible it is, but in the first image, there are no droplets, but the surface is still moist, there is water captured underneath the mycelium (also there is a droplet right next to the pins). After the misting, there visible small droplets of water all over the myc. Should I have misted, or should I wait for it to dry out more?

Second question is, why are the pins growing only in one corner of the tub?

I'm not complaining, like I said at the start, even if I get one mushroom out if it I'm happy, I'm just wondering can I do something to improve the pinning conditions?


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27338382 - 06/07/21 04:46 AM (11 days, 8 hours ago)

As pins move in you can cut down misting.
Surface has good moisture.
Maybe conditions in the corner were a bit better than the rest of the surface.
Could be more moisture from condensation running down the walls.
Next time look to get a proper surface like in the link:
Proper Surface Moisture / When to Increase FAE
After pinning, the moisture content should be enough til harvest,
unless you get a monster pinset you may have to support with bottom watering.

There´s a lot more knotting, you`re on it :thumbup:

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Re: First ever spawning to sub, any advice would be helpful [Re: Goatrider]
    #27338400 - 06/07/21 05:16 AM (11 days, 7 hours ago)

Yup, got most of my info about surface conditions from that tek. The biggest mistake I made was that I didn't tamp down the sub enough when I was spawning. In the beginning it was just a bunch of small bumps, but as mycelium grew underneath and around them, those chunks of sub moved and now it's really bumpy. Next time I will definitely focus more to get everything level and pat it down.

Question about bottom watering, or dunking after fist flush. I have a liner, and myc is really holding on to it now. If hypothetically I had to bottom water (or dunk), should I unstick the liner from the myc so that the sub is free from all sides?


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27338473 - 06/07/21 07:25 AM (11 days, 5 hours ago)

Quote:

Te0nanacatl said:
If hypothetically I had to bottom water (or dunk), should I unstick the liner from the myc so that the sub is free from all sides?




Exactly this.



Quote:

Te0nanacatl said:
The biggest mistake I made was that I didn't tamp down the sub enough when I was spawning.Next time I will definitely focus more to get everything level and pat it down.




You`re on the right track mate :wink:

            :cookiemonster:


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Re: First ever spawning to sub, any advice would be helpful [Re: Goatrider]
    #27342494 - 06/10/21 06:22 AM (8 days, 6 hours ago)

I have a mixed bag of news.

Good news is the mushrooms are still growing, I'm trying to keep the surface conditions wet enough. The weird part is the big ones are all leaning towards the ground. Some of the pins are not growing for a few days now and their caps are a bit darker, maybe they are aborts.



The bad news is I'm 90% sure veil has ripped on my biggest mushroom, and the cap isn't even expanded, so I have no idea whats happening there.

Here is a bonus shitty gif of a shroom growing over 2 hour period, I'm surprised they grow so fast. :mushroom2: :mushroom2: :mushroom2:



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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27342697 - 06/10/21 10:41 AM (8 days, 2 hours ago)

ahhhh bummer dude. looks like that spawn was dirty after all. sorry


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Re: First ever spawning to sub, any advice would be helpful [Re: Bakedbeings]
    #27342776 - 06/10/21 11:25 AM (8 days, 1 hour ago)

It looks like the RH is too high. When its too high I find my mushrooms get kinda soggy and fall over like that. What are you fruiting those in?

If in a tub you can flip the lid to create a small crack around the edge.

I'd harvest the big ones and see if you can get the smaller ones to start growing normally.


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Edited by PitcherCrab (06/10/21 11:28 AM)


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Re: First ever spawning to sub, any advice would be helpful [Re: PitcherCrab]
    #27342868 - 06/10/21 12:16 PM (8 days, 49 minutes ago)

Quote:

Bakedbeings said:
ahhhh bummer dude. looks like that spawn was dirty after all. sorry



What makes you think this is a result of a contamination?

Quote:

PitcherCrab said:
If in a tub you can flip the lid to create a small crack around the edge.




It's a shoebox, with an unclipped lid, so that should provide enough FAE. Do you think I should flip the lid to increase it?

I've been misting once per day, once I don't see the droplets on the surface, I mist 5-6 pumps. Should I stop completely?


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27342985 - 06/10/21 01:51 PM (7 days, 23 hours ago)

last year i had a few jars that werent sterilized properly but they looked ok so i spawned them anyway. colonized beautifully, produced a solid pinset, but then they started curling inward and popping veils prematurely. they were infected with some very sneaky bacteria

its also possible that your spawn was clean but the mycelium was simply weakened from sitting in the jar for so long. idk im not a mycologist


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Re: First ever spawning to sub, any advice would be helpful [Re: Bakedbeings]
    #27343037 - 06/10/21 02:23 PM (7 days, 22 hours ago)

Yeah stop misting completely, thats way too much for a shoebox. I like to double tub mine — put a second shoebox on top of the first — and offset them a little so there is a crack around the edge. I find that give a good amount of air. The only time I mist or bottom water is between flushes. Unless its looking super dry, then I'll make an exception.


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Re: First ever spawning to sub, any advice would be helpful [Re: PitcherCrab]
    #27343093 - 06/10/21 02:58 PM (7 days, 22 hours ago)

Quote:

Bakedbeings said:
last year i had a few jars that werent sterilized properly but they looked ok so i spawned them anyway. colonized beautifully, produced a solid pinset, but then they started curling inward and popping veils prematurely. they were infected with some very sneaky bacteria

its also possible that your spawn was clean but the mycelium was simply weakened from sitting in the jar for so long. idk im not a mycologist



Did you still harvest them and eat them or did you toss the whole tub?
From what I read it seems like its fine to eat even the pins that came from contaminated tubs/cakes, is that correct?

Quote:


PitcherCrab said:
Yeah stop misting completely, thats way too much for a shoebox. I like to double tub mine — put a second shoebox on top of the first — and offset them a little so there is a crack around the edge. I find that give a good amount of air. The only time I mist or bottom water is between flushes. Unless its looking super dry, then I'll make an exception.




Yeah, I guess I fucked that up. The funny part is that I read how the inexperienced people mist their first tubs too much, while I was misting my tub too much.
But you said it yourself - "Unless its looking super dry", I guess I don't get what is super dry? Am I correct in thinking that you should see beads of water on mycelium?
Should I fan it now to increase evaporation, or just dub-tub it and avoid touching it?


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27343152 - 06/10/21 03:35 PM (7 days, 21 hours ago)

Just double tub them and leave them alone. They should bounce back and recover once things start to dry out a bit, and you'll probably get more pins from all that moisture evaporating. There should be beads of water on the mycelium during pinning, but after that it's not as important. A dead giveaway of dryness is if any coir peeking up through the mycelium is dry, and no condensation at all on the sides of your tub.


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Re: First ever spawning to sub, any advice would be helpful [Re: PitcherCrab]
    #27345412 - 06/12/21 12:54 PM (6 days, 11 minutes ago)

Latest development is that there is a handful of new pins on the completely other side of the tub, and they are growing super fast. There is still condensation on the side of the box, so I didn't mist for three days.



I'm worried that they don't look too healthy. The big one has fuzzy feet, that could be due to lack of FAE, but I'm not sure. I'm worried about stem splitting, since the first thing I found when I searched for it is that it could be a vert infection (lecanicillium). I also found a post from RR saying that it could be due to rapid growth, and they are growing fast. They were pins when I stopped misting, and they grew to this size in less than 36 hours. This is a timelaps of 2 hours of growth.



So the questions are: Should I increase FAE with fanning? Is the stem splitting sign of something serious? As you can see in the last picture, I have a bunch of aborts with dark caps, it looks like I drowned them during my misting phase, they haven't grown a mm in days. Should I pick them now before they start to rot?


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27345449 - 06/12/21 01:23 PM (5 days, 23 hours ago)

Quote:


Did you still harvest them and eat them or did you toss the whole tub?




i tossed the whole tub. im sure the fruits were edible but they were so tiny and gross looking and most of them were burrowed into the sub and i just couldnt be bothered to salvage them when i had healthy myc fruiting in the next tub and a gallon ziploc of dry beauties sitting in my drawer


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Re: First ever spawning to sub, any advice would be helpful [Re: Bakedbeings]
    #27345473 - 06/12/21 01:44 PM (5 days, 23 hours ago)

Fuzzy feet means too much RH typically, but I wouldn't worry about that it's not a huge deal. I wouldn't fan, just leave the lid cracked and you'll be good. The splitting stems are likely from fast growth as RR said. What is the temperature like on these? High temps speed up growth.

If it were me I'd pick the old gross aborts and make sure the whole has plenty of overhead light to give the baby pins a direction to grow in. The way they are growing all willy-nilly indicates you might not be giving them enough light. Then let it do it's thing. I looks like everything is heading in a good direction. Based on how fast the big ones are growing it looks like you should harvest them soon.


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Re: First ever spawning to sub, any advice would be helpful [Re: PitcherCrab]
    #27346293 - 06/13/21 03:47 AM (5 days, 9 hours ago)

Quote:

PitcherCrab said:
Fuzzy feet means too much RH typically, but I wouldn't worry about that it's not a huge deal. I wouldn't fan, just leave the lid cracked and you'll be good. The splitting stems are likely from fast growth as RR said. What is the temperature like on these? High temps speed up growth.

If it were me I'd pick the old gross aborts and make sure the whole has plenty of overhead light to give the baby pins a direction to grow in. The way they are growing all willy-nilly indicates you might not be giving them enough light. Then let it do it's thing. I looks like everything is heading in a good direction. Based on how fast the big ones are growing it looks like you should harvest them soon.




The temperature is pretty stable and not extremely high, it varies from 26-28c (79-82.5f). The humidity in the room is pretty high though, I haven't seen it drop below 45%, and it mostly sits above 50%.
As for the light I'm not sure if I fucked it up somehow, but I've got a 13w led light, 6500k, 1280 lumens, and I keep it on 12/12 regiment. Based on everything I read, it should be the correct light. But as you can see in the gif, they are definitely turning towards the light, so maybe it's doing something.



Also, I noticed this morning that it looks like both of these two long ones have dropped their spores overnight, so I fucked that up also. Should I pick them now, and can I still get a spore print from them? I plan on picking the aborts today, since it looks like that 90% of the pins that were present while I was misting daily have aborted.


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27346309 - 06/13/21 03:59 AM (5 days, 9 hours ago)

Quote:

Te0nanacatl said:
Should I pick them now, and can I still get a spore print from them?




Yes, they should shoot some more spores the next ~48 hours.
And yes, pick the aborts.

            :cookiemonster:


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Re: First ever spawning to sub, any advice would be helpful [Re: Goatrider]
    #27346329 - 06/13/21 04:59 AM (5 days, 8 hours ago)

Ok, I will do a partial harvest and pick the big ones so I can print them, and all of the aborts. Hopefully, I get some more growth out of these new ones. I just can't believe I destroyed 50% of my pins and yield with over misting...
Thank you for your help everyone, I would've gotten 0 mushrooms if I didn't have this thread :mushroom2:


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27346784 - 06/13/21 02:47 PM (4 days, 22 hours ago)

yes you can still get spores from those


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Re: First ever spawning to sub, any advice would be helpful [Re: Bakedbeings]
    #27346823 - 06/13/21 03:24 PM (4 days, 21 hours ago)

Ya live ya learn... It's a bit counter intuitive. I can't tell you how many pins I've aborted from over misting. Waaay too many.


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Re: First ever spawning to sub, any advice would be helpful [Re: PitcherCrab]
    #27348843 - 06/15/21 08:58 AM (3 days, 4 hours ago)

I harvested all of the mushrooms and the aborts for a massive harvest of ~50g wet  :yeahthatsfunny: But considering where I started I can't complain.

This is the current look of my shoebox. Dark purple spots are places where the spores dropped. The sub is separating from the liner on the sides, so I plan on unsticking it completely and pouring a cup of tap water down between the liner and the sub, heavily misting the surface, and see if anything will happen. I'll also keep it on the same 12/12 light cycle.
Should I do something else, more/less water?


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27348944 - 06/15/21 10:37 AM (3 days, 2 hours ago)

Heavily mist so there is standing water. Then let it absorb over night and repeat the following day. Then let it do it's thing!


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“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear


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Re: First ever spawning to sub, any advice would be helpful [Re: PitcherCrab]
    #27350479 - 06/16/21 12:12 PM (2 days, 53 minutes ago)

I misted heavily yesterday, and everything was soaked up today during the day. The issue is a bunch of pins started popping up today during to day, it looks like they were hiding under the surface.



Now I don't know if I should mist it heavily again tonight, or since pins started popping up just leave it as is?


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Re: First ever spawning to sub, any advice would be helpful [Re: Te0nanacatl]
    #27350593 - 06/16/21 01:41 PM (1 day, 23 hours ago)

My vote is leave them. Surface is looking plenty wet, and you've got pins and a bunch of primordia so let them do their thing.


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“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear


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