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OfflineGriff89
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Registered: 01/03/21
Posts: 57
Last seen: 3 years, 4 months
Need opinions on spore use...
    #27315457 - 05/19/21 06:57 PM (3 years, 7 months ago)

Hello so here is my dilemma. 

I have 3 syringes left with roughly 4ml left in each.

Recently completed a very successful BRF cake harvest but now left with roughly 12ml of spore but not sure if i wanna do cakes again.

What woud be your 2 cents on using and getting the best harvest for these?

Thanks in advance!

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OfflineSplendidSwimmer
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Registered: 05/19/21
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Last seen: 1 year, 5 months
Re: Need opinions on spore use... [Re: Griff89]
    #27315462 - 05/19/21 07:08 PM (3 years, 7 months ago)

If you're getting harvests, it's pretty easy to take spore prints. Not sure why you'd stress about what is left in your syringe

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Offlinewoodandwicker
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Registered: 05/19/21
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Last seen: 3 years, 7 months
Re: Need opinions on spore use... [Re: SplendidSwimmer] * 1
    #27315464 - 05/19/21 07:10 PM (3 years, 7 months ago)

Make a liquid culture (e.g. karo tek).

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OfflineGriff89
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Registered: 01/03/21
Posts: 57
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Re: Need opinions on spore use... [Re: woodandwicker]
    #27315472 - 05/19/21 07:14 PM (3 years, 7 months ago)

I unfortunately didnt take any prints as i was just excited to be filling my goal jar. So now everything is dehydrated and i have syringes left.

Liquid culture. Would this be basically thinning my spore solution i have and making more?

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InvisibleMycelium Juice
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Registered: 10/31/20
Posts: 513
Loc: planet earth
Re: Need opinions on spore use... [Re: woodandwicker] * 2
    #27315502 - 05/19/21 07:53 PM (3 years, 7 months ago)

Don't make a liquid culture with a spore syringe before cleaning it up an agar first.  That's just asking for trouble.
It will give any contamination present a wonderful environment to overtake your mycelium and render your LC useless.

Spores are inherently dirty due to the environment they come from.
Best way to ensure a successful grow would be cleaning up your syringes on agar first. 

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OfflineCocaineBuffet
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Re: Need opinions on spore use... [Re: woodandwicker] * 4
    #27315504 - 05/19/21 07:53 PM (3 years, 7 months ago)

Quote:

woodandwicker said:
Make a liquid culture (e.g. karo tek).




Don't do this with spores. Take that shit back to reddit

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InvisibleAshtray161
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Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
Re: Need opinions on spore use... [Re: CocaineBuffet] * 1
    #27315508 - 05/19/21 08:00 PM (3 years, 7 months ago)

for real, no spores to LC
put those spores to agar and use them as practice.


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Invisiblemicelio
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Re: Need opinions on spore use... [Re: Griff89]
    #27315515 - 05/19/21 08:07 PM (3 years, 7 months ago)

I'm with everyone....  AGAR


--------------------
     

Edited by micelio (05/19/21 08:08 PM)

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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
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Re: Need opinions on spore use... [Re: micelio] * 1
    #27315539 - 05/19/21 08:27 PM (3 years, 7 months ago)

:whathesaid:



Agar is the answer.

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InvisibleMilk-away
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Registered: 02/04/21
Posts: 249
Re: Need opinions on spore use... [Re: verum subsequentis]
    #27315749 - 05/20/21 12:42 AM (3 years, 7 months ago)

Make BRF pucks/half-pint jars
Squirt your spores into those
Wait for the most beautiful rhizomorphic (stringy) mycelium to form
Open the jars in an SAB and with sterile tools, scoop some of the mycelium into your Liquid Culture jar.
Make liquid culture by mixing 400ml water with 16g honey/corn-syrup and pressure cooking the jar.
Wait for the small BRF grain to colonize your LC jar (3-5 days)
Open the LC jar in an SAB and using a sterile syringe (that you flame sterilized the needle of) suck some LC.
Store the syringes in the fridge.
You can use them to colonize 1quart pressure sterilized grain jars in 5-10 days


--------------------
...Let go...

Edited by Milk-away (05/20/21 12:43 AM)

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InvisiblebodhisattaMDiscordReddit
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Re: Need opinions on spore use... [Re: CocaineBuffet]
    #27315880 - 05/20/21 05:00 AM (3 years, 7 months ago)

Quote:

CocaineBuffet said:
Quote:

woodandwicker said:
Make a liquid culture (e.g. karo tek).




Don't do this with spores. Take that shit back to reddit



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Offlineblackout
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Registered: 07/16/00
Posts: 5,266
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Re: Need opinions on spore use... [Re: Griff89]
    #27316332 - 05/20/21 01:01 PM (3 years, 7 months ago)

Quote:

Griff89 said:
Hello so here is my dilemma. 

I have 3 syringes left with roughly 4ml left in each.

Recently completed a very successful BRF cake harvest but now left with roughly 12ml of spore but not sure if i wanna do cakes again.




I get 25 drops per ml from syringes (some sites say 20 drops per ml but I have measured 25). I put 2 drops in each jar now, so your 12ml would be 150 inoculations for me.

Obviously if you are flaming needles you will be frying them, and needing to let them cool etc.

It needs good syringe control to put out just a drop or 2, I have given tips on this before.

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