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Offlinelittlenemo
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Registered: 05/20/04
Posts: 13
Loc: London UK
Last seen: 14 years, 18 days
100% contam only from 1 syringe
    #2731576 - 05/26/04 11:24 AM (14 years, 1 month ago)

I recently got hold of 2 spore syringes, thai and golden teacher strains. I inoculated all containers in the same sterile glovebox setup using exactly the same procedure. While every container inoculated w the thai syringe is fine, every single one of those inoculated with the golden teacher syringe turned yellow/brown within 24 hours and now shows some kind of green coloured mold.

My question- is this strain much more susceptible to contams or does this indicate something else, ie an already contaminated syringe?

Thanks...


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InvisibleJim
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Registered: 04/07/04
Posts: 20,898
Re: 100% contam only from 1 syringe [Re: littlenemo]
    #2731651 - 05/26/04 11:59 AM (14 years, 1 month ago)

maybe you got a syringe from a contaminated spore print.


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Offlinelittlenemo
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Registered: 05/20/04
Posts: 13
Loc: London UK
Last seen: 14 years, 18 days
Re: 100% contam only from 1 syringe [Re: Jim]
    #2731689 - 05/26/04 12:09 PM (14 years, 1 month ago)

Thanks, yeah this is what i felt had happened. seems unlikely that anything else could be to blame...


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OfflineCptnGarden
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Registered: 05/13/04
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Re: 100% contam only from 1 syringe [Re: littlenemo]
    #2731731 - 05/26/04 12:26 PM (14 years, 1 month ago)

...


Edited by CptnGarden (03/29/09 12:49 AM)


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OfflineMAIA
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Re: 100% contam only from 1 syringe [Re: littlenemo]
    #2731923 - 05/26/04 01:45 PM (14 years, 1 month ago)

Yeap, blame the syringe ...

MAIA


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Offlinelittlenemo
Stranger
Registered: 05/20/04
Posts: 13
Loc: London UK
Last seen: 14 years, 18 days
Re: 100% contam only from 1 syringe [Re: MAIA]
    #2732680 - 05/26/04 04:22 PM (14 years, 1 month ago)

thanks for the feedback, at least now I can be pretty sure my procedure was ok and that this strain is not necessarily more susceptible to contams... which is good news as I like the effect of this strain... I'll just have to try again!


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