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Offlineclintster442
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Registered: 07/31/20
Posts: 19
Loc: Orbiting Pluto
Last seen: 3 years, 5 months
First Attempt at Agar
    #27315473 - 05/19/21 07:18 PM (3 years, 7 months ago)

This is my first attempt at agar. I decided to use the premade agar plates from Amazon but I also bought all the stuff to make my own agar dishes for the next time.

I inoculated the dishes last Saturday evening (4 days ago) using spore syringes and already have what looks to be great mycelium growing. The tiny black specs are the spores.

My last attempt at growing, I tried to inoculate sterile grain bags straight the spore syringe and was unsuccessful so this is my first time getting mycelium to actually grow.

Being that, I don’t see anything that looks like mold or infection. The mycelium is very white and fluffy. I didn’t have any parafilm so I used plastic wrap (which I doubt is doing anything at all to maintain sterility).

A lot of room for improvement in my process but I’m happy with the progress and learning a lot along the way.

Strains are entheogenic explosion and Air Force one. The EE is doing much better than the AF1 but both are growing nicely.

I plan to do my grain spawn in the next 10-14 days or so.

Any tips or feedback greatly appreciated.



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OfflineTheMagicConch
MC
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Registered: 09/11/20
Posts: 841
Loc: Over here
Last seen: 1 year, 2 months
Re: First Attempt at Agar [Re: clintster442]
    #27315486 - 05/19/21 07:30 PM (3 years, 7 months ago)

A suggestion for wrapping the dishes would be to take glad cling wrap (if you don't want to buy parafilm) and you can cut a section the height of the dish off of the roll and use that to wrap your petris.

Also look into a pyrex media bottle for mixing your own agar. All you gotta do is add the dry ingredients, add the water, mix it up at much as you can. Loosen the lid so it doesn't explode in your microwave and just heat it up a minute at a time, take it out and swirl, repeat until it's all mixed up. Cover the top in foil (lid still loosened to allow for air exchange) Then just throw it in your PC (following the directions for your PC model as far as how much water to use etc) for 20-30 minutes.

Those media bottles pour really nice and you get to skip the boiling water and mixing in a pot on the stove etc.

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InvisibleAshtray161
SettledNomad
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Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
Re: First Attempt at Agar [Re: TheMagicConch]
    #27315499 - 05/19/21 07:47 PM (3 years, 7 months ago)

yeah i just made my first set of plates and its way easier than it seems at first


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