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Griff89

Registered: 01/03/21
Posts: 57
Last seen: 3 years, 4 months
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Need opinions on spore use...
#27315457 - 05/19/21 06:57 PM (3 years, 7 months ago) |
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Hello so here is my dilemma.
I have 3 syringes left with roughly 4ml left in each.
Recently completed a very successful BRF cake harvest but now left with roughly 12ml of spore but not sure if i wanna do cakes again.
What woud be your 2 cents on using and getting the best harvest for these?
Thanks in advance!
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SplendidSwimmer
Stranger

Registered: 05/19/21
Posts: 11
Last seen: 1 year, 5 months
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Re: Need opinions on spore use... [Re: Griff89]
#27315462 - 05/19/21 07:08 PM (3 years, 7 months ago) |
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If you're getting harvests, it's pretty easy to take spore prints. Not sure why you'd stress about what is left in your syringe
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woodandwicker
Stranger
Registered: 05/19/21
Posts: 3
Last seen: 3 years, 7 months
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Make a liquid culture (e.g. karo tek).
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Griff89

Registered: 01/03/21
Posts: 57
Last seen: 3 years, 4 months
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I unfortunately didnt take any prints as i was just excited to be filling my goal jar. So now everything is dehydrated and i have syringes left.
Liquid culture. Would this be basically thinning my spore solution i have and making more?
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Mycelium Juice
Here for the weed



Registered: 10/31/20
Posts: 513
Loc: planet earth
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Don't make a liquid culture with a spore syringe before cleaning it up an agar first. That's just asking for trouble. It will give any contamination present a wonderful environment to overtake your mycelium and render your LC useless.
Spores are inherently dirty due to the environment they come from. Best way to ensure a successful grow would be cleaning up your syringes on agar first.
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CocaineBuffet
Stranger



Registered: 08/29/19
Posts: 4,271
Last seen: 19 hours, 53 minutes
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Quote:
woodandwicker said: Make a liquid culture (e.g. karo tek).
Don't do this with spores. Take that shit back to reddit
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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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for real, no spores to LC put those spores to agar and use them as practice.
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micelio
Song of Silence


Registered: 04/22/14
Posts: 1,547
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Re: Need opinions on spore use... [Re: Griff89]
#27315515 - 05/19/21 08:07 PM (3 years, 7 months ago) |
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I'm with everyone.... AGAR
Edited by micelio (05/19/21 08:08 PM)
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 2 years, 7 months
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Re: Need opinions on spore use... [Re: micelio] 1
#27315539 - 05/19/21 08:27 PM (3 years, 7 months ago) |
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Agar is the answer.
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Milk-away
'Mmkay fortyseven



Registered: 02/04/21
Posts: 249
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Make BRF pucks/half-pint jars Squirt your spores into those Wait for the most beautiful rhizomorphic (stringy) mycelium to form Open the jars in an SAB and with sterile tools, scoop some of the mycelium into your Liquid Culture jar. Make liquid culture by mixing 400ml water with 16g honey/corn-syrup and pressure cooking the jar. Wait for the small BRF grain to colonize your LC jar (3-5 days) Open the LC jar in an SAB and using a sterile syringe (that you flame sterilized the needle of) suck some LC. Store the syringes in the fridge. You can use them to colonize 1quart pressure sterilized grain jars in 5-10 days
-------------------- ...Let go...
Edited by Milk-away (05/20/21 12:43 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Quote:
CocaineBuffet said:
Quote:
woodandwicker said: Make a liquid culture (e.g. karo tek).
Don't do this with spores. Take that shit back to reddit
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 1 year, 2 months
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Re: Need opinions on spore use... [Re: Griff89]
#27316332 - 05/20/21 01:01 PM (3 years, 7 months ago) |
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Quote:
Griff89 said: Hello so here is my dilemma.
I have 3 syringes left with roughly 4ml left in each.
Recently completed a very successful BRF cake harvest but now left with roughly 12ml of spore but not sure if i wanna do cakes again.
I get 25 drops per ml from syringes (some sites say 20 drops per ml but I have measured 25). I put 2 drops in each jar now, so your 12ml would be 150 inoculations for me.
Obviously if you are flaming needles you will be frying them, and needing to let them cool etc.
It needs good syringe control to put out just a drop or 2, I have given tips on this before.
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