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InvisibleAshtray161
SettledNomad
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Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
When Knocking Up BRF
    #27314486 - 05/19/21 02:40 AM (3 years, 7 months ago)

I was just thinking, when shooting spore solution into BRF jars, wont the drop coming out right as ur about to stick the needle in each hole (after spritzing out a tiny bit to cool down the needle after flaming) be a vector for contamination even in a SAB?
Would it be better to stick a hot needle in the jar or would that just cause too much burnt substrate to stick onto the needle?


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"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
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InvisibleGoatrider
Rhythm Guitarist
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Registered: 04/08/20
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Re: When Knocking Up BRF [Re: Ashtray161]
    #27314490 - 05/19/21 02:53 AM (3 years, 7 months ago)

After flaming just wait a few seconds,
and get the needle in. It cools as you push liquid.
Angle the syringe to let the needle hit the glass.

                :cookiemonster:


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Invisiblestubb
Dahg Rastubfari
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Registered: 03/23/19
Posts: 1,447
Loc: Memory Flag
Re: When Knocking Up BRF [Re: Ashtray161]
    #27314502 - 05/19/21 03:01 AM (3 years, 7 months ago)

Quote:

Ashtray161 said:
wont the drop coming out right as ur about to stick the needle in each hole (after spritzing out a tiny bit to cool down the needle after flaming) be a vector for contamination even in a SAB?




No, that drop just traveled through a red hot needle.  Even the spores in that drop are dead.

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InvisibleAshtray161
SettledNomad
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Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
Re: When Knocking Up BRF [Re: stubb]
    #27314505 - 05/19/21 03:02 AM (3 years, 7 months ago)

Quote:

stubb said:
Quote:

Ashtray161 said:
wont the drop coming out right as ur about to stick the needle in each hole (after spritzing out a tiny bit to cool down the needle after flaming) be a vector for contamination even in a SAB?




No, that drop just traveled through a red hot needle.  Even the spores in that drop are dead.




ah, yea i guess thats a good point. But then again with the syringes ive noticed they just kinda keep dripping after they start


--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086:elmo:
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
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Offlinejimineycricket
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Registered: 09/26/19
Posts: 77
Last seen: 6 months, 27 days
Re: When Knocking Up BRF [Re: Ashtray161]
    #27314507 - 05/19/21 03:04 AM (3 years, 7 months ago)

Quote:

Ashtray161 said:
I was just thinking, when shooting spore solution into BRF jars, wont the drop coming out right as ur about to stick the needle in each hole (after spritzing out a tiny bit to cool down the needle after flaming) be a vector for contamination even in a SAB?
Would it be better to stick a hot needle in the jar or would that just cause too much burnt substrate to stick onto the needle?




I think you might be over thinking it.I believe Flaming the needle isn't to sterilize the spore solution coming out. You just have to hope the spore solution was made in as clean a process as possible. Flaming the needle kills any contams from the air that have landed on the needle and that may land on the needle for a little while after while its still hot. Dont worry too much about burned substrate on the needle . I have a tissue soaked in isopropyl alcohol on a plate ready to wipe off any substrate on needle after each jar before I flame sterilize again.

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Invisiblestubb
Dahg Rastubfari
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Registered: 03/23/19
Posts: 1,447
Loc: Memory Flag
Re: When Knocking Up BRF [Re: Ashtray161]
    #27314513 - 05/19/21 03:08 AM (3 years, 7 months ago)

Quote:

Ashtray161 said:
But then again with the syringes ive noticed they just kinda keep dripping after they start




After you figure out how to kinda finesse them, they stop doing that.  Unfortunately I can't really articulate what that means, it's a muscle memory thing.  What I can say is that you have far more control if you hold your syringe like this:


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InvisibleGoatrider
Rhythm Guitarist
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Registered: 04/08/20
Posts: 4,801
Loc: Germany Flag
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Re: When Knocking Up BRF [Re: stubb]
    #27314528 - 05/19/21 03:26 AM (3 years, 7 months ago)

:whathesaid:

Let your index finger act as a brake to the plunger.

              :cookiemonster:


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OfflineDERRAYLD
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Registered: 05/13/02
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Re: When Knocking Up BRF [Re: Goatrider]
    #27314534 - 05/19/21 03:34 AM (3 years, 7 months ago)

I squirt a tiny release qty and flame sterilize after

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InvisibleMilk-away
'Mmkay fortyseven
I'm a teapot User Gallery


Registered: 02/04/21
Posts: 249
Re: When Knocking Up BRF [Re: DERRAYLD]
    #27314631 - 05/19/21 06:02 AM (3 years, 7 months ago)

I love squirting spores
:spideysplooge:

But I flame my needle between every stab of the BRF.. I don't want a wet needle going and wetting my dry verm layer on top of the cake :wink:


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