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OfflineDbehan
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Registered: 05/16/21
Posts: 1
Loc: Colorado Flag
Last seen: 3 years, 2 months
Where to inoculate
    #27311500 - 05/16/21 06:07 PM (3 years, 7 months ago)

Hi. I’m new to the site and growing. I’ve done a few monotubs so far. My first one was perfect and yielded quite a bit. The last couple I’ve done have gotten mold and even more recently my spawn has been getting green mold.

I’ve been using rye seed for my spawn and usually sterilize my own. I recently bought some from out-grow. I cleaned my bathroom, sterilized the spawn bags, needles and my gloves. Then I injected a bag and immediately put duct tape over the white injection tape. I did this on each bag, re-sterilizing my needle and each bag. So far every bag I inoculated has grown mold with mycelium. So I am kind of lost. I am going to make a plastic “clean room” with a zipper on another part of my house. But it’s weird that my first grow went so well and now I can’t even grown clean mycelium 🤣.

Any help would be appreciated. Thanks!


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InvisibleSmartattack
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Registered: 12/21/18
Posts: 3,788
Loc: A thought
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Re: Where to inoculate [Re: Dbehan]
    #27311511 - 05/16/21 06:15 PM (3 years, 7 months ago)

Its not your room, its the fact you are working with syringes to grain. (manage to get to say this once a day) look up agar teks and leave that garb process behind you.


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OfflineJulius orange

Registered: 11/09/18
Posts: 62
Last seen: 2 years, 4 months
Re: Where to inoculate [Re: Smartattack]
    #27311519 - 05/16/21 06:19 PM (3 years, 7 months ago)

:whathesaid:
Also open air in a bathroom certainly isn't where you should inoculate. Use the handy search function, check out what's called a still air box or SAB.

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InvisibleAshtray161
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Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
Re: Where to inoculate [Re: Julius orange]
    #27311603 - 05/16/21 07:21 PM (3 years, 7 months ago)

agar is where its at my man, if u can make ur own jars u can make agar

READ THIS: https://www.shroomery.org/forums/showflat.php/Number/21922023


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Edited by Ashtray161 (05/16/21 07:22 PM)

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OfflineJosborne
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Registered: 11/18/20
Posts: 184
Last seen: 3 years, 4 months
Re: Where to inoculate [Re: Ashtray161]
    #27311870 - 05/16/21 10:04 PM (3 years, 7 months ago)

When you say syringe I'm assuming you mean MS? MS should really only be used for agar dishes as some of the posters above said and pf tek/brf jars. You should expect MS syringes to have contaminants since you can't produce them in a 100% sterile way.

Do MS to agar and then transfer. Or you can use the MS for brf cakes and clone the fruits (which you would also do on agar).

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InvisibleGoatrider
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Registered: 04/08/20
Posts: 4,801
Loc: Germany Flag
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Re: Where to inoculate [Re: Josborne]
    #27311968 - 05/17/21 12:01 AM (3 years, 7 months ago)

Quote:

Josborne said:
you can't produce them in a 100% sterile way




Here`s a sponsor that produces very high quality syringes.
It`s some effort you usually don`t see in my opinion.
Nevertheless you should never shoot direct to grains as mentioned.



Imagine i`d have gone straight to grains with this :wink:
That`s why we use agar. A few transfers and done.

                :cookiemonster:


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InvisibleMycelium Juice
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Registered: 10/31/20
Posts: 513
Loc: planet earth
Re: Where to inoculate [Re: Dbehan]
    #27312024 - 05/17/21 01:45 AM (3 years, 7 months ago)

Quote:

Dbehan said:

I’ve been using rye seed for my spawn and usually sterilize my own. I recently bought some from out-grow...So far every bag I inoculated has grown mold with mycelium. So I am kind of lost....

Any help would be appreciated. Thanks!




I can help!
Don't buy pre-made grain spawn.  Ever.:firecum:
Proof is in the pudding- or in your case bag of mold and the wasted time, money and spores. 

The recipe for success is simple if people would just stick to it.  Agar->grains->fruit those fuckers. 

No pre-made shortcuts that cost 20x more than doing it yourself and usually have abysmal results.
No just sticking spore syringes in things and praying.

Make your own grain spawn and agar plates. 
If you fail, learn from your failures and try until you get it right.

We're here to help, but there's nothing to learn about pre-made anything here except don't buy it.
Everyone else got you on the agar tip already

Good luck! :mushroom2:

Edited by Mycelium Juice (05/17/21 01:59 AM)

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InvisibleGoatrider
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Male


Registered: 04/08/20
Posts: 4,801
Loc: Germany Flag
Trusted Cultivator
Re: Where to inoculate [Re: Mycelium Juice]
    #27312030 - 05/17/21 01:55 AM (3 years, 7 months ago)

:whathesaid:

I`d advise against bags for the start, it`s a bit advanced.
They`re best done G2G or inoculated with liquid inoculant (li)
or liquid culture (lc).
In your case it`s not just the premade stuff you have to trust in,
but also in the spores you shoot in.
Great chances on failing mate.
I always recommend this flowchart here to get an idea on further processing:



                      :cookiemonster:


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