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OfflineMissWhitney
Female
Registered: 09/12/23
Posts: 10
Loc: Southern Oregon
Last seen: 1 month, 1 day
Re: Post Your Gourmet Cultivation Picture of the Day [Re: Mushroom love]
    #28486024 - 09/28/23 05:49 PM (3 months, 28 days ago)

Don’t think I labeled it incorrectly, but there’s always a chance lol. I’ll find out eventually I hope, put it to grain jar last night.


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Offline0ptiquest
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Registered: 07/05/20
Posts: 509
Last seen: 7 days, 6 hours
Re: Post Your Gourmet Cultivation Picture of the Day [Re: MissWhitney]
    #28486038 - 09/28/23 06:05 PM (3 months, 28 days ago)

Yeah that pins look like oysters.


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OfflineOm Namo Shivaya


Registered: 05/03/15
Posts: 749
Last seen: 8 days, 10 hours
Re: Post Your Gourmet Cultivation Picture of the Day [Re: 0ptiquest]
    #28486410 - 09/29/23 04:36 AM (3 months, 28 days ago)

Yup, definitely not Lion's mane pins on that plate.


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OfflineMissWhitney
Female
Registered: 09/12/23
Posts: 10
Loc: Southern Oregon
Last seen: 1 month, 1 day
Re: Post Your Gourmet Cultivation Picture of the Day [Re: Om Namo Shivaya]
    #28486478 - 09/29/23 07:41 AM (3 months, 28 days ago)

Thanks for all the input, I’m new, these were my first gourmet clones. So I can learn, what makes you think this is oyster? I see similar pinning when googling, Google image search. Thanks in advance just trying to gain some knowledge!


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Offline0ptiquest
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Registered: 07/05/20
Posts: 509
Last seen: 7 days, 6 hours
Re: Post Your Gourmet Cultivation Picture of the Day [Re: MissWhitney]
    #28486517 - 09/29/23 08:27 AM (3 months, 28 days ago)

Similar but your pins have little heads, so probably oyters.


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Offline0ptiquest
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Registered: 07/05/20
Posts: 509
Last seen: 7 days, 6 hours
Re: Post Your Gourmet Cultivation Picture of the Day [Re: 0ptiquest]
    #28490178 - 10/02/23 05:57 PM (3 months, 24 days ago)

good practice to resterilize this supplemented blocks or nah?


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InvisibleMushroom love
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Registered: 04/06/12
Posts: 99
Loc: Europe
Re: Post Your Gourmet Cultivation Picture of the Day [Re: 0ptiquest] * 4
    #28492171 - 10/04/23 03:56 PM (3 months, 23 days ago)

During my last attempts at growing Cordyceps, I learned that single ascospore isolate (liquid culture from 02/2021) can be kept much longer alone without senescence than crossed. The substrate was completely colonized by a single ascospore culture then I inoculated with a clone (degenerated) then placed in fruiting conditions. I'm happy with the result, I took a few clones on agar and I'm going to try to isolate more single ascospore.

Here is the degenerate clone alone:


Here, full colonization with different old single ascospore (2021) then add the degenerate clone or ms from this clone:




The best cross exceeds 100% BE. 27g of rice per container:



Today's total harvest:


I was hesitant to order a new liquid culture, I did well to try to use these old single ascospore isolates from the fridge.


--------------------
All my work is done without SAB or LFH, just a Bunsen burner


Edited by Mushroom love (10/04/23 04:11 PM)


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Offlinedeadmandave
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Registered: 02/16/10
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Re: Post Your Gourmet Cultivation Picture of the Day [Re: Mushroom love]
    #28492370 - 10/04/23 07:07 PM (3 months, 22 days ago)

Really cool. Where did the single ascospore isolate come from?


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InvisibleMushroom love
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Registered: 04/06/12
Posts: 99
Loc: Europe
Re: Post Your Gourmet Cultivation Picture of the Day [Re: deadmandave]
    #28492755 - 10/05/23 03:55 AM (3 months, 22 days ago)

Quote:

deadmandave said:
Really cool. Where did the single ascospore isolate come from?



I purchased a fruiting culture from William Padilla Brown in 2020 (and two more from Jeff Manganaro in 2021). From this culture I isolated around forty single ascospores which I tested and I kept the 8 which gave me the best results. Of these 8, 4 are still vigorous (2 years and 7 months old). I am really surprised because cultures able of fruiting (2 single ascospore compatible mixed together) degenerate very quickly in general (in 3 to 6 months). I isolated the spores on water agar with a Cordyceps fixed on the lid with petroleum jelly. I don't have a microscope to confirm spore isolation so I tested each spore on a small substrate (in test tubes):
- if it produces fruiting, it is not an isolated spore but several.
- if it doesn't fruit then it is either several incompatible spores or successful isolation.
Then I tested different isolates that did not fruit alone but fruited together. This gave me testers that I called "+" and "-".
Now, when I isolate a spore, I try to make it fruit alone, with a "+" tester and with a "-" tester.
Another thing that lets you know that the isolation was successful is the particular appearance of each culture on agar.


--------------------
All my work is done without SAB or LFH, just a Bunsen burner


Edited by Mushroom love (10/05/23 04:10 AM)


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InvisibleQenwi
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Registered: 04/15/23
Posts: 10
Re: Post Your Gourmet Cultivation Picture of the Day [Re: Mushroom love]
    #28494428 - 10/06/23 01:12 PM (3 months, 21 days ago)

These cordyceps look nice. Is it set and forget tek? The way you grow the i mean


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InvisibleMushroom love
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Registered: 04/06/12
Posts: 99
Loc: Europe
Re: Post Your Gourmet Cultivation Picture of the Day [Re: Qenwi]
    #28494610 - 10/06/23 04:23 PM (3 months, 21 days ago)

Quote:

Qenwi said:
These cordyceps look nice. Is it set and forget tek? The way you grow the i mean



This was a test but yes it's brown rice + a nutrient solution, all sterilized for 45 minutes then inoculated with a liquid culture. Cultivation is done without opening the container until harvest (approximately 55-60 days after inoculation)


--------------------
All my work is done without SAB or LFH, just a Bunsen burner


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OfflineOm Namo Shivaya


Registered: 05/03/15
Posts: 749
Last seen: 8 days, 10 hours
Re: Post Your Gourmet Cultivation Picture of the Day [Re: Mushroom love] * 3
    #28495616 - 10/07/23 04:09 PM (3 months, 20 days ago)

Little update from my small grow. These g. Sessile are starting to form pores and maybe are conking out? Is that unusual for reishi to do?


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InvisibleMushroom love
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Registered: 04/06/12
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Loc: Europe
Re: Post Your Gourmet Cultivation Picture of the Day [Re: Om Namo Shivaya] * 1
    #28496370 - 10/08/23 10:25 AM (3 months, 19 days ago)

I don't see any pores but I could be wrong. Can you take a zoom photo on it? My Reishi also made some kind of balls at the beginning:

Your other grows are pretty, I love your Piopinno


--------------------
All my work is done without SAB or LFH, just a Bunsen burner


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OfflineOm Namo Shivaya


Registered: 05/03/15
Posts: 749
Last seen: 8 days, 10 hours
Re: Post Your Gourmet Cultivation Picture of the Day [Re: Mushroom love]
    #28497275 - 10/09/23 05:26 AM (3 months, 18 days ago)



It’s only the sessile putting pores out. These are pores right?


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Offlinegt40
I will proof smthng
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Registered: 09/17/12
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Re: Post Your Gourmet Cultivation Picture of the Day [Re: Mushroom love]
    #28498969 - 10/10/23 03:16 PM (3 months, 17 days ago)

Quote:

Mushroom love said:
The substrate was completely colonized by a single ascospore culture then I inoculated with a clone (degenerated) then placed in fruiting conditions.



do you mean that you mate single ascospore with a clone on agar then fruiy it or what?


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InvisibleMushroom love
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Registered: 04/06/12
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Loc: Europe
Re: Post Your Gourmet Cultivation Picture of the Day [Re: gt40]
    #28499649 - 10/11/23 05:21 AM (3 months, 16 days ago)

Quote:

Om Namo Shivaya said:


It’s only the sessile putting pores out. These are pores right?



In this photo it looks more like pores. My reishi have also formed some but they have not produced spores yet
Quote:

gt40 said:
Quote:

Mushroom love said:
The substrate was completely colonized by a single ascospore culture then I inoculated with a clone (degenerated) then placed in fruiting conditions.



do you mean that you mate single ascospore with a clone on agar then fruiy it or what?




I inoculated a brown rice substrate with a single ascospore isolate and about 15 days later I inoculated the completely colonized substrate with the liquid culture of the clone. I then waited 5 days before putting it into fruiting condition. The substrate became much more orange after adding the clone.


--------------------
All my work is done without SAB or LFH, just a Bunsen burner


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OfflineMissWhitney
Female
Registered: 09/12/23
Posts: 10
Loc: Southern Oregon
Last seen: 1 month, 1 day
Re: Post Your Gourmet Cultivation Picture of the Day [Re: gt40] * 3
    #28500101 - 10/11/23 12:58 PM (3 months, 16 days ago)

You guys were right, my labels were wrong! So I believe it was a king oyster, and here is my actual lionsmane 😅


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Offlinegt40
I will proof smthng
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Registered: 09/17/12
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Re: Post Your Gourmet Cultivation Picture of the Day [Re: Mushroom love]
    #28501062 - 10/12/23 12:08 AM (3 months, 15 days ago)

Quote:

Mushroom love said:
I inoculated a brown rice substrate with a single ascospore isolate and about 15 days later I inoculated the completely colonized substrate with the liquid culture of the clone. I then waited 5 days before putting it into fruiting condition. The substrate became much more orange after adding the clone.



thanks and wow! how you decided to do it?


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InvisibleMushroom love
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Registered: 04/06/12
Posts: 99
Loc: Europe
Re: Post Your Gourmet Cultivation Picture of the Day [Re: gt40]
    #28501154 - 10/12/23 03:33 AM (3 months, 15 days ago)

Quote:

gt40 said:
Quote:

Mushroom love said:
I inoculated a brown rice substrate with a single ascospore isolate and about 15 days later I inoculated the completely colonized substrate with the liquid culture of the clone. I then waited 5 days before putting it into fruiting condition. The substrate became much more orange after adding the clone.



thanks and wow! how you decided to do it?



The idea for this test came to me while reading this  thread. And these 2 other splendid thread: here and here.


--------------------
All my work is done without SAB or LFH, just a Bunsen burner


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InvisibleMushroom love
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Registered: 04/06/12
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Re: Post Your Gourmet Cultivation Picture of the Day [Re: Mushroom love] * 2
    #28503105 - 10/13/23 10:28 AM (3 months, 14 days ago)

Today's harvest :



Mono dubtubed 32L



A bag in SGFC


--------------------
All my work is done without SAB or LFH, just a Bunsen burner


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