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Offlinemoshe1

Registered: 08/29/15
Posts: 378
Last seen: 2 years, 7 months
[AGAR/] Fuzzy/Cottony and Transparent Growth on Agar (mold?)
    #27311287 - 05/16/21 02:34 PM (3 years, 7 months ago)


Edit : it seems there are a lot of differing opinions so i'll update and clean up information as discussion continues and I test things out myself. I have updated with higher-res photos and just pinning them here as well.


are these indications of contam? about half my plates have completely flat semitransparent growth on agar, other half have fuzzy spots like these.  Other threads i've found that are close to the same question seem to be pointing to healthy but just want to make sure since its such a 50/50 split from all my Transfer 1s.







Edit 1 : updated pictures of germ plates and different transfers 05/17



heres a general overhead shot of some of my plates and transfers below them


a couple close ups of blue meanie germ plates. one of them has not been opened since germination


blue meanie fuzzy transfer 1 growth top and bottom views.


here is a blue meanie plate not showing much of the fuzzy- almost all thin and transparent. in-light and on table view to get a good idea



LGT germ plate and Transfer 1 growth


MSS PE6 germ plate and transfer 1 growth, both transparent and flat, and also with fuzz


a clone taken from a pin via biopsy- from very late generation myc



Edited by moshe1 (05/19/21 05:08 PM)

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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: agar flat vs fuzzy growth [Re: moshe1]
    #27311296 - 05/16/21 02:40 PM (3 years, 7 months ago)

Are them grain soak agar?
What was the mixture? How much nutrient to how much water to how much agar?
Is that plate with all the writting a germination plate?

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Offlinemoshe1

Registered: 08/29/15
Posts: 378
Last seen: 2 years, 7 months
Re: agar flat vs fuzzy growth [Re: Feasoghorm]
    #27311348 - 05/16/21 03:18 PM (3 years, 7 months ago)

Quote:

Feasoghorm said:
Are them grain soak agar?
What was the mixture? How much nutrient to how much water to how much agar?
Is that plate with all the writting a germination plate?





no not grain soak. Just LME and Agar about 7-8 grams per 10 grams agar. About the same as Bods agar recipe for ~20 plates. pretty much roughly going off that.

yes the scribbled one is germ plates, almost all germ plates look like that more or less. Theres a lot more these are just examples didnt wanna spam with pics


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Edited by moshe1 (05/16/21 06:08 PM)

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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: agar flat vs fuzzy growth [Re: moshe1]
    #27311361 - 05/16/21 03:26 PM (3 years, 7 months ago)

Not exactly sure what does that or why. However it most certainly looks fucked up and unhealthy.

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Offlinemoshe1

Registered: 08/29/15
Posts: 378
Last seen: 2 years, 7 months
Re: agar flat vs fuzzy growth [Re: Feasoghorm]
    #27311508 - 05/16/21 06:12 PM (3 years, 7 months ago)

ill continue transferring anyway- thanks for the reply

my inexperienced eye makes me assume that its tementose growth- with some support from threads like these https://www.shroomery.org/forums/showflat.php/Number/12213222


heres some more examples/ views






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Edited by moshe1 (05/16/21 06:24 PM)

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InvisibleAshtray161
SettledNomad
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Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
Re: agar flat vs fuzzy growth [Re: moshe1]
    #27311610 - 05/16/21 07:27 PM (3 years, 7 months ago)

Quote:

moshe1 said:
ill continue transferring anyway- thanks for the reply

my inexperienced eye makes me assume that its tementose growth- with some support from threads like these https://www.shroomery.org/forums/showflat.php/Number/12213222


heres some more examples/ views









Im getting identical growth patterns on mine too.
Im not sure what its all about... Maybe nutrient content in the agar?
At first I thought I must have a bunch of mold but Ive done a total of 5 control plates. 3/5 were left open the whole time I was working during a session and I rubbed my gloves across them at the end to see what was on em. 1 of them was opened for the session and not touched at the end, the last one was left in the SAB for a few hours during a day that i wasnt working in it.

All 5 grew tiny spots of bacteria (and on a couple of the plates the bacteria got super big and awesome looking) but only ONE plate grew mold and it was very obvious black mold that turned colors about 48hours after spotting growth and its pitch black when it turns so its definitely not that.
Mind you, my controls still could of missed something from a vector they didnt test but id think if ALL my plates had this weird whispy white mold then at least one of my controls would pick it up and Ive gotten nothing of the sort.


--------------------
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OfflineJulius orange

Registered: 11/09/18
Posts: 62
Last seen: 2 years, 4 months
Re: agar flat vs fuzzy growth [Re: Ashtray161]
    #27311623 - 05/16/21 07:35 PM (3 years, 7 months ago)

Try using distilled water for your agar. I moved and had weak growth on known shitty "don't drink from the tap" city water. In the past I've used tap just fine and plenty of others do, but hey it's worth a shot.

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Offlinemoshe1

Registered: 08/29/15
Posts: 378
Last seen: 2 years, 7 months
Re: agar flat vs fuzzy growth [Re: Julius orange]
    #27311989 - 05/17/21 12:39 AM (3 years, 7 months ago)

so what you're saying is the water i use to prep the agar could be causing growth to be a certain way? What would be the explanation it be exactly? I have some distilled sitting around i could try


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Edited by moshe1 (05/17/21 12:40 AM)

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Offlinehazyhorse
scoobin
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Registered: 03/19/19
Posts: 3,822
Last seen: 2 months, 5 days
Re: agar flat vs fuzzy growth [Re: moshe1]
    #27311996 - 05/17/21 12:46 AM (3 years, 7 months ago)

Quote:

moshe1 said:
my inexperienced eye makes me assume that its tementose growth- with some support from threads like these https://www.shroomery.org/forums/showflat.php/Number/12213222






the thread you linked to is on oyster mushroom mycelium, it looks a lot different than cubensis mycelium. itโ€™s a lot more whispy. tomentose cube myc definitely doesnโ€™t look like your plates, sorry /: theyโ€™re looking pretty suspect to me. they should at the very least be somewhat white but that growth just looks sus. unless the clear agar is fucking with the visibility that bad but... idk should be a LITTLE thicker than that even on clear agar


--------------------
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Offlinemoshe1

Registered: 08/29/15
Posts: 378
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Re: agar flat vs fuzzy growth [Re: hazyhorse]
    #27312019 - 05/17/21 01:35 AM (3 years, 7 months ago)

i didn't notice- regardless there are other threads.

https://www.shroomery.org/forums/showflat.php/Number/26986824#26986824

check this one out, also RR commenting saying there are no obvious contams/molds and the plates look sort of like mine.

all of the transfers that dont have the thick white puffy growths on them are pretty thin and translucent actually- across all 4 varieties i have on transfer one rn. not seeing any "thick" myc except on those germ plates but those look all over the place at this point- a lot of growth with those thick and fluffier growths around



heres some other germ plates with T1



also some initial germs




Edited by moshe1 (05/17/21 02:00 AM)

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InvisibleMilk-away
'Mmkay fortyseven
I'm a teapot User Gallery


Registered: 02/04/21
Posts: 249
Re: agar flat vs fuzzy growth [Re: moshe1]
    #27312031 - 05/17/21 02:00 AM (3 years, 7 months ago)

Try getting a more diverse nutrient base for your agar, maybe add 2g PDA to the mix and a ml of coffee.
I had the same problem, then made upped the nutes by 30%(by weight, adding Yeast and coffee and BRF to a classic PDA) and then my mycelium turned the thickest white and looked very good.
But it might just be me..


--------------------
...Let go...

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Offlinemoshe1

Registered: 08/29/15
Posts: 378
Last seen: 2 years, 7 months
Re: agar flat vs fuzzy growth [Re: Milk-away] * 1
    #27312034 - 05/17/21 02:11 AM (3 years, 7 months ago)

interesting- i was on the hunch that nutes were too high and i should cut some malt to see more aggressive growth. i could do both and get a some sort of answer

ill take some pics and try to get high-res linked here tomorrow these are all a couple days old


--------------------

Edited by moshe1 (05/17/21 02:13 AM)

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Offlinehazyhorse
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Registered: 03/19/19
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Re: agar flat vs fuzzy growth [Re: moshe1]
    #27312087 - 05/17/21 03:53 AM (3 years, 7 months ago)

huh. weird, youโ€™re probably fine then. ignore me lol


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===================================
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posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

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OfflineJulius orange

Registered: 11/09/18
Posts: 62
Last seen: 2 years, 4 months
Re: agar flat vs fuzzy growth [Re: moshe1]
    #27312167 - 05/17/21 06:03 AM (3 years, 7 months ago)

Quote:

moshe1 said:
so what you're saying is the water i use to prep the agar could be causing growth to be a certain way? What would be the explanation it be exactly? I have some distilled sitting around i could try




1,2,4-trichlorobenzene, chlorine. I don't know exactly. I don't know the specific effects of various water contaminants on mycelium. I can tell you that in my case switching to distilled made a huge difference.

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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 2,329
Re: agar flat vs fuzzy growth [Re: Julius orange]
    #27312207 - 05/17/21 06:51 AM (3 years, 7 months ago)

Ime, clear shiny halo is mold
Grow it out?

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Offlinemoshe1

Registered: 08/29/15
Posts: 378
Last seen: 2 years, 7 months
Re: agar flat vs fuzzy growth [Re: LadysKnight]
    #27312381 - 05/17/21 10:28 AM (3 years, 7 months ago)

honestly gonna grow it out and see what happens- trying to get some plates to pin so might as well

Quote:

RogerRabbit said:
Quote:

Clc420 said:







I've seen this before.  As said above, it could be your agar is too nutrient dense.  Make several transfers to new plates a with a bit less nutrients and see how it progresses.  I see no bacteria or obvious mold.
RR





gonna go off this and make next batch with less nutes- then try the latter and adding stuff into agar. i like simplest solutions.


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InvisibleAntsInYourPants

Registered: 01/27/21
Posts: 99
Re: agar flat vs fuzzy growth [Re: LadysKnight]
    #27312403 - 05/17/21 10:46 AM (3 years, 7 months ago)

Quote:

LadysKnight said:
Ime, clear shiny halo is mold
Grow it out?




My experience is the opposite. The clear halo is usually new growth that hasn't fully filled out whenever I see it on my plates. Growing it out is always the solution though. If you wanna make sure transfer some of that clear growth to a new plate and I bet you'll have mycelium. Great way to learn.

It could be nutrients or genetics. Agar plates can look wild on the first few transfers. You might wanna do a few more transfers before you start changing the nutrients up, sometimes they can just look like that. I never worried about how they look as long as they were clean personally. You can't tell how somethings going to fruit based off an Agar plate.

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Offlinemoshe1

Registered: 08/29/15
Posts: 378
Last seen: 2 years, 7 months
Re: agar flat vs fuzzy growth [Re: AntsInYourPants]
    #27312662 - 05/17/21 02:44 PM (3 years, 7 months ago)

i've got some better pictures to check out across all the strains i have going.  was uploading a different file format and these pics are much, much better.
Most of my plates are from a Blue Meanie print, the others are from a LGT print, and a couple from a Maz print. Got one MSS PE6 dish going as well-


heres a general overhead shot of some of my plates and transfers below them


a couple close ups of blue meanie germ plates. one of them has not been opened since germination


blue meanie fuzzy transfer 1 growth top and bottom views.


here is a blue meanie plate not showing much of the fuzzy- almost all thin and transparent. in-light and on table view to get a good idea



LGT germ plate and Transfer 1 growth


MSS PE6 germ plate and transfer 1 growth, both transparent and flat, and also with fuzz


a clone taken from a pin via biopsy- from very late generation myc



I also have a couple transfers from a Mazy germ plate which has the same thin, transparent layer of myc.

Edited by moshe1 (05/17/21 02:51 PM)

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