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Offlinematrix7y7y
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could you help this first timer select the right agar plate
    #27309370 - 05/15/21 05:36 AM (2 years, 8 months ago)

Hi guys, could you help me select the right agar plate?

i'm trying to get it right this time



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Invisiblesandman420
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Re: could you help this first timer select the right agar plate [Re: matrix7y7y]
    #27309410 - 05/15/21 06:43 AM (2 years, 8 months ago)

I am sorry to inform you that these are all 100% mold.

Are the black globs spores?


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OfflineTheMycoLab
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Re: could you help this first timer select the right agar plate [Re: sandman420]
    #27309417 - 05/15/21 06:49 AM (2 years, 8 months ago)

none of that looks normal bud


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Offlinematrix7y7y
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Re: could you help this first timer select the right agar plate [Re: TheMycoLab]
    #27309854 - 05/15/21 01:24 PM (2 years, 8 months ago)

yes these are spores from GT and did it in a SAB. :confused:


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Invisiblesandman420
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Re: could you help this first timer select the right agar plate [Re: matrix7y7y]
    #27309867 - 05/15/21 01:33 PM (2 years, 8 months ago)

either the print is bad or you scraped the spores with a fork you found in the dumpster behind Long John Silvers.


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InvisibleAshtray161
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Re: could you help this first timer select the right agar plate [Re: sandman420]
    #27309935 - 05/15/21 02:50 PM (2 years, 8 months ago)

Quote:

sandman420 said:
either the print is bad or you scraped the spores with a fork you found in the dumpster behind Long John Silvers.



:whathesaid:
I say this with all good intent and no malleolus but perhaps its time to do some rereading mate? Get an idea of what healthy myc looks like on a plate cuz those (except maybe pic #2) should all stick out as pretty obviously fucked.


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Offlinematrix7y7y
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Re: could you help this first timer select the right agar plate [Re: Ashtray161]
    #27310782 - 05/16/21 06:58 AM (2 years, 8 months ago)

how about these? these showed slower growth.


is it so that contamination grows allot quicker?



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Invisiblemyc_ousin_vinny
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Re: could you help this first timer select the right agar plate [Re: matrix7y7y]
    #27310797 - 05/16/21 07:10 AM (2 years, 8 months ago)

I can't tell if the black spots are mold or spores but they look pretty rough too.


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Invisiblegive.more
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Re: could you help this first timer select the right agar plate [Re: matrix7y7y]
    #27310800 - 05/16/21 07:13 AM (2 years, 8 months ago)

I've noticed that many syringes have charcoal added for some reason. I had a problem with black spots more recently when germinating on agar.

I'd go ahead and do a transfer in a SAB while sanitizing between each, just in case. Germinating spores on agar have always looked sus to me. Then add to it that the caps for those cups can distort the image.

Pay attention to what each cup looks like and you'll start to notice more of what to look for.

And Ashtray is right, #2 in the OP is the only one that looks good. The second set of pics aren't clear enough to tell. You want white growth that's not discoloring with age.


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Edited by give.more (05/16/21 07:20 AM)


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OfflineRogerRabbitM
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Re: could you help this first timer select the right agar plate [Re: matrix7y7y]
    #27310848 - 05/16/21 08:00 AM (2 years, 8 months ago)

Spore syringes were developed for use with brf cakes.  They don't do well on agar because the contaminants are favored.  If you're going to use plates, it's best to use a spore print, and then swipe the spores onto the plate in a zig-zag formation so you can grab individual mycelium growths before the contamination has a chance to gain a foothold.

Try inoculating brf cakes and then isolate from mature fruits which develop.
RR


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Offlinematrix7y7y
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Re: could you help this first timer select the right agar plate [Re: give.more]
    #27310850 - 05/16/21 08:02 AM (2 years, 8 months ago)

it's spores scraped with a disinfected knife in a SAB, i have to admit its probably too heavily scraped onto the agar. it was dificult to controll the knife in my home built SAB. its probably also the reason for my other agar plates to be contaminated as i put soo much spores.


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Offlinematrix7y7y
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Re: could you help this first timer select the right agar plate [Re: matrix7y7y]
    #27310852 - 05/16/21 08:04 AM (2 years, 8 months ago)

i have a spore print


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Invisiblemyc_ousin_vinny
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Re: could you help this first timer select the right agar plate [Re: RogerRabbit]
    #27312115 - 05/17/21 04:55 AM (2 years, 8 months ago)

:takingnotes:
Quote:

RogerRabbit said:
Spore syringes were developed for use with brf cakes.  They don't do well on agar because the contaminants are favored.  If you're going to use plates, it's best to use a spore print, and then swipe the spores onto the plate in a zig-zag formation so you can grab individual mycelium growths before the contamination has a chance to gain a foothold.

Try inoculating brf cakes and then isolate from mature fruits which develop.
RR





:takingnotes:  What's up RR! :cheers:


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