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Jinxmate
Permafried



Registered: 06/14/20
Posts: 57
Loc: Australia
Last seen: 3 years, 5 months
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Contam check for a LC noob
#27309526 - 05/15/21 08:31 AM (3 years, 7 months ago) |
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Hey people, first off I know you shouldn't ever use MSS with LC. I got a magnetic stirrer and made a LC honey jar (480ml water, 21g honey, PC for 20-25 mins @ 15psi taken out PC ASAP) to use it with because I wanted to see it spin lol. Only just getting all my agar equip now so I couldn't use an agar slice, I used 0.5ml from a APE-LC sticker from a reputable vendor, he said this culture is a good agar plate to LC. I have already nocced 12 jars and spawned a tub from this culture. Posting in hopes some people experienced with LC/agar can pitch in and tell me if its a waste to spawn this or not, Cheers.
  
   This is what worry me this grey bit on the stirrer is stays intact after stirring and doesn't grow, possible contam?

APE Jars from same culture. Growth is so strong its reaching up and growing on just glass, bit of mycporn.
    
    

Compared to GT started on 3/4, Started 8 days before APE!

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kanemush
Grumpy

Registered: 12/07/17
Posts: 1,564
Last seen: 17 days, 1 hour
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Re: Contam check for a LC noob [Re: Jinxmate]
#27309609 - 05/15/21 09:36 AM (3 years, 7 months ago) |
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Hate to tell you we really can't know by looking if a LC is contaminated or not. Now people can give an opinion but only agar tells us the truth. That being said some people say if its cloudy it's no good. So I don't have a for sure answer for you without testing it on agar.
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Jinxmate
Permafried



Registered: 06/14/20
Posts: 57
Loc: Australia
Last seen: 3 years, 5 months
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Re: Contam check for a LC noob [Re: kanemush]
#27309633 - 05/15/21 09:46 AM (3 years, 7 months ago) |
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I got some plates, I just need to figure out how to make them up properly. I have an idea but havent seen a proper recipe, I only have Light malt extract and agar agar powder. Have to do some on those once I make em up. Cheers mate
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kanemush
Grumpy

Registered: 12/07/17
Posts: 1,564
Last seen: 17 days, 1 hour
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Re: Contam check for a LC noob [Re: Jinxmate]
#27309767 - 05/15/21 12:00 PM (3 years, 7 months ago) |
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5g lme and 5g agar-agar mix with 250ml water done recipe easy pc and have fun
Edited by kanemush (05/15/21 12:00 PM)
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Jinxmate
Permafried



Registered: 06/14/20
Posts: 57
Loc: Australia
Last seen: 3 years, 5 months
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Re: Contam check for a LC noob [Re: kanemush]
#27309864 - 05/15/21 01:31 PM (3 years, 7 months ago) |
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Thanks alot mate ill get on that ASAP. 
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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Re: Contam check for a LC noob [Re: kanemush]
#27309939 - 05/15/21 02:52 PM (3 years, 7 months ago) |
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Quote:
kanemush said: Hate to tell you we really can't know by looking if a LC is contaminated or not. Now people can give an opinion but only agar tells us the truth. That being said some people say if its cloudy it's no good. So I don't have a for sure answer for you without testing it on agar.
Personally I have a cloudy LC thats awesome and grows clean on agar. I also had a clear LC that I thought was gonna be good but was nothing but mold and bacteria. You can never tell til u put that shit on agar lol
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