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Timestop413
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Does trich come through dirty grain work or can it come through a casing layer? Looking for help
#27304301 - 05/11/21 12:59 PM (3 years, 8 months ago) |
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Hi,
I'm trying to get my cultivation back on track after dealing with a good bit of trich this round. I stored a master culture of penis envy in my refrigerator for a few months and used it to start up some new agar dishes. Admittedly I have noticed on those new agar dishes, little tiny bits of trichoderma spawning as a dish was nearly fully grown out, so a plate looked nearly perfect but then at the last day or so before using it..little bit of trich popped up around the center, it took me a bit to catch this, so I think when I went to do agar to grain, some trich got into some Master jars, it still managed to colonize but i then spread those to some other jars and alot contaminated. Some of the jars seemed to colonize fine, however..now that I'm fruiting those jars in a mono tub, seeing more trich come up, **specifically after I add the casing layer once the substrate is 100% colonized. Using jiffy mix pc'd for 60 minutes as a casing.
My question is, do you think the trichoderma is coming In from the agar/grains/substrate work or is it more than likely coming in from the air/environment and taking hold once I pop the tub open to add the casing layer? I remember seeing someone say "99% of a tubs success comes down to the grain work, convince me I'm wrong" (it was that meme with the guy at the table in the park drinking coffee with the sign in front of him that makes a statement and says "convince me I'm wrong or tell me otherwise" sorta deal.
I know there is a fair bit of trichoderma in my current environment/house for what its worth but I do have a flow hood and have been able to do some clean culture work from that master culture since I've noticed the trich popping up on those transfers, took a few isolations away from it but I have some clean cultures currently. I also have a few tubs (small portion of the room) that I've cased and gotten two flushes out and they aren't showing any trich signs, so it makes me wonder/think that it could be more of a grain work issue but I really dont know.
My thinking/hoping is that...if there is maybe small amounts of trich in the grains/substrate, once that casing layer goes on top..it has uncolonized material that it can take a hold of before the mycelium gets it. My other thought, less ideal because I dont know how to resolve it, is that my environment is just dirty and there's to much trichoderma in the air and thats why things are contaminating.
Anyone have any experience or input they can share? I appreciate it!
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bodhisatta 
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Registered: 04/30/13
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Loc: Milky way
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Re: Does trich come through dirty grain work or can it come through a casing layer? Looking for help [Re: Timestop413]
#27304305 - 05/11/21 01:06 PM (3 years, 8 months ago) |
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You literally said its on your agar. And then asked where it's from. Its from your sterile technique 
If its on your agar its in all your work until you get clean agar
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Land Trout
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Re: Does trich come through dirty grain work or can it come through a casing layer? Looking for help [Re: bodhisatta]
#27304403 - 05/11/21 02:25 PM (3 years, 8 months ago) |
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Trichoderma is probably present in every inch of soil on earth, and also in every airmass in the lower atmosphere. So it doesn’t “come from” your grain or agar, it got to your agar, grain or whatever because of a lack sterile of technique. Trich also dies at a pretty low temp, likely all trich spores die as soon as your pc starts to vent. It gets into your jar or plate once you open it. And if your spawn is good your cubensis will outcompete whatever trich gets into your tub when you spawn it, maybe not if it’s a large spore load, but I’m sure trich spores end up in all our tubs when we spawn them. It only takes hold if conditions are better for trich than they are cubes.
Edited by Land Trout (05/11/21 02:27 PM)
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jomanda1990
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Re: Does trich come through dirty grain work or can it come through a casing layer? Looking for help [Re: Land Trout]
#27304552 - 05/11/21 04:21 PM (3 years, 8 months ago) |
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Though the problem probably comes from dirty spawn, I think you shouldn't sterilize the casing layer either, only pasteurize it.
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Timestop413
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Re: Does trich come through dirty grain work or can it come through a casing layer? Looking for help [Re: bodhisatta]
#27309120 - 05/14/21 10:44 PM (3 years, 7 months ago) |
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Quote:
bodhisatta said: You literally said its on your agar. And then asked where it's from. Its from your sterile technique 
If its on your agar its in all your work until you get clean agar
right I did thats true, I appreciate it. I think I was more trying to figure out if I had totally clean grain spawn, but a dirty environment, would it contam or be fine, But I know I need to clean up the agar work regardless.
I'm surprised to see the trich continue to pop up several transfers away from the initial clone dish,...its not popping up on the outer portion of the agar dish, it's faintly becoming visible right in the center of the dish underneath a nice plate of mycelium once the dish is basically fully grown out. I have some clean spores that I started due to this and I've been transferring for a bit now, they aren't showing contam.
I've been taking transfers from the outer perimeter of dishes, I didnt expect the trich to make it into the new dishes selecting from the furthest out portion of growth, it must just be hidden right there because if its starting right in the center, makes me think the selected piece has it on there.
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Ashtray161
SettledNomad



Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Re: Does trich come through dirty grain work or can it come through a casing layer? Looking for help [Re: Timestop413]
#27309168 - 05/15/21 12:02 AM (3 years, 7 months ago) |
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Quote:
Timestop413 said:
Quote:
bodhisatta said: You literally said its on your agar. And then asked where it's from. Its from your sterile technique 
If its on your agar its in all your work until you get clean agar
right I did thats true, I appreciate it. I think I was more trying to figure out if I had totally clean grain spawn, but a dirty environment, would it contam or be fine, But I know I need to clean up the agar work regardless.
I'm surprised to see the trich continue to pop up several transfers away from the initial clone dish,...its not popping up on the outer portion of the agar dish, it's faintly becoming visible right in the center of the dish underneath a nice plate of mycelium once the dish is basically fully grown out. I have some clean spores that I started due to this and I've been transferring for a bit now, they aren't showing contam.
I've been taking transfers from the outer perimeter of dishes, I didnt expect the trich to make it into the new dishes selecting from the furthest out portion of growth, it must just be hidden right there because if its starting right in the center, makes me think the selected piece has it on there.
Trich and mushroom mycel look very similar before the trich sporulates and turns green. Once you see green theres more white trich in ur plates ur not realizing is trich and likely moving it over every transfer. That or once youve noticed the trich its already sporeulated obviously and ur moving mushroom myc covered in trich spores
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Land Trout
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Re: Does trich come through dirty grain work or can it come through a casing layer? Looking for help [Re: Ashtray161]
#27309596 - 05/15/21 09:22 AM (3 years, 7 months ago) |
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If you see green on your plate you can assume you have spore all over your plate. You want to transfer before you see green, but sounds like you might have an easier time starting over, or at least I would, but I’m lazy and don’t have time to work that hard. I’ve heard of a few tricks to beat contamns, but the best method is transfer before it matures and spores.
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Timestop413
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Re: Does trich come through dirty grain work or can it come through a casing layer? Looking for help [Re: Land Trout]
#27309823 - 05/15/21 12:37 PM (3 years, 7 months ago) |
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Quote:
Land Trout said: If you see green on your plate you can assume you have spore all over your plate. You want to transfer before you see green, but sounds like you might have an easier time starting over, or at least I would, but I’m lazy and don’t have time to work that hard. I’ve heard of a few tricks to beat contamns, but the best method is transfer before it matures and spores.
Ok that's helpful, I know what you're talking about in regards to it being white before it goes green, have been paying more attention to it and that makes sense. I'll see what I can do.
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