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InvisibleRandyBobandy
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Help with cleaning up a few plates * 1
    #27309760 - 05/15/21 11:50 AM (3 years, 7 months ago)

Hey all! I put some tissue to agar a week ago and now I’m gonna clean them up (assuming I have mycelium here.) The 3 clones are pictured below. Apologies if the pics are not clear enough I attempted to get them as clear as possible.







Now notice the white dots mixed w the mycelium (specifically in the 3rd pic.) I’m assuming it’s some sort of contam but my agar had “air bubbles” in it and the mycelium grew right over where some of those bubbles were located. Looks suspicious to me but I figured I might add that.

Also recommendations on where I should transfer on each plate would be appreciated. Thanks!


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Edited by RandyBobandy (05/15/21 12:07 PM)

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OfflineTheMagicConch
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Re: Help with cleaning up a few plates [Re: RandyBobandy]
    #27309808 - 05/15/21 12:26 PM (3 years, 7 months ago)

The dots on the 3rd pic just look like myc getting fluffy in the air bubbles.

Plate#1- just a hair to the right of 6 o'clock
Plate#2- 7 o'clock
Plate#3- personally I wouldn't transfer anything from this, the other 2 plates have a couple other areas with stronger growth that you could do a second transfer from, but if you're still trying to get at least one transfer from all of the plates, go with 11 o'clock

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InvisibleRandyBobandy
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Registered: 01/29/19
Posts: 141
Loc: Mushroom Mountain
Re: Help with cleaning up a few plates [Re: TheMagicConch]
    #27310020 - 05/15/21 04:07 PM (3 years, 7 months ago)

Quote:

TheMagicConch said:
The dots on the 3rd pic just look like myc getting fluffy in the air bubbles.

Plate#1- just a hair to the right of 6 o'clock
Plate#2- 7 o'clock
Plate#3- personally I wouldn't transfer anything from this, the other 2 plates have a couple other areas with stronger growth that you could do a second transfer from, but if you're still trying to get at least one transfer from all of the plates, go with 11 o'clock




Appreciate the reply! Have any experience with panaeolus or Psilocybe Mexicana? I took some spores to agar and would like to know if I have some decent growth ready to be transferred. . First time with both species.







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OfflineTheMagicConch
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Re: Help with cleaning up a few plates [Re: RandyBobandy]
    #27310106 - 05/15/21 05:02 PM (3 years, 7 months ago)

I don't have any experience with those, but basically all you wanna do is transfer from the area thats growing the fastest. I would give those a couple more days so you can see the different sectors more definitively and choose the fastest/healthiest looking area

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