Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
OfflineJosborne
Stranger
Registered: 11/18/20
Posts: 184
Last seen: 3 years, 4 months
Why agar transfers getting weaker? * 1
    #27308433 - 05/14/21 01:46 PM (3 years, 8 months ago)




I've been doing multiple transfers from the same culture and the last batch all came out looking like the bottom out (good growth in some places, weak looking growth in others) even though the master plate looked like the top one (uniform dense growth).

Many of the plates in the new batch look even worse than the bottom one pictured with all/most of the growth is very weak/whispy looking

Is this something to be worried about?

Edited by Josborne (05/14/21 01:47 PM)

Extras: Filter Print Post Top
Invisibleseldom seen
April Fool
Male User Gallery


Registered: 11/03/07
Posts: 1,103
Re: Why agar transfers getting weaker? [Re: Josborne]
    #27308442 - 05/14/21 01:51 PM (3 years, 8 months ago)

How many transfers?  Maybe switch up the media?


--------------------

Extras: Filter Print Post Top
OfflineJosborne
Stranger
Registered: 11/18/20
Posts: 184
Last seen: 3 years, 4 months
Re: Why agar transfers getting weaker? [Re: seldom seen]
    #27308490 - 05/14/21 02:20 PM (3 years, 8 months ago)

Quote:

seldom seen said:
How many transfers?  Maybe switch up the media?





This is maybe the fifth "batch?" What I've been doing is I transferred from the best looking master plate into 10-15 or so receiving plates. Then the same for the best looking plate of the second batch. And so on. So effectively I've done 70 or so plates from one original master plate/culture.

Do you mean changing agar to water ratios or using something besides agar? What do you use?

Edited by Josborne (05/14/21 02:22 PM)

Extras: Filter Print Post Top
OfflineJosborne
Stranger
Registered: 11/18/20
Posts: 184
Last seen: 3 years, 4 months
Re: Why agar transfers getting weaker? [Re: Josborne]
    #27308500 - 05/14/21 02:27 PM (3 years, 8 months ago)

Also at first I thought this might be senescence, but after a little reading it seems like the consensus on this forum is that senescence takes a long long time to happen and is not understood very well anyway.

Is that green light to transfer, clone, g2g, fruit, etc as much as I want? Or will I eventually have to go back to spores? (speaking within a year timeframe)

Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,387
Trusted Cultivator
Re: Why agar transfers getting weaker? [Re: Josborne] * 1
    #27308517 - 05/14/21 02:37 PM (3 years, 8 months ago)

How big of a batch of agar do you make at one time? (ml)

A possible explanation could be that less genetics per transfer equals less dense growth.

Or maybe you messed up your agar recipe last round and that made a lot of weak plates possibly?


I mean both of those plates look just fine to me. But you said you had a whole batch of whispy ones so...


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Extras: Filter Print Post Top
Offlinesmalltalk_canceled
Babnik
 User Gallery

Registered: 07/13/20
Posts: 3,174
Last seen: 9 hours, 40 minutes
Re: Why agar transfers getting weaker? [Re: sandman420]
    #27308536 - 05/14/21 02:45 PM (3 years, 8 months ago)

Why is the bottom plate not good? Weak growth what


--------------------
Willpower is the one true virtue


Extras: Filter Print Post Top
OfflineJosborne
Stranger
Registered: 11/18/20
Posts: 184
Last seen: 3 years, 4 months
Re: Why agar transfers getting weaker? [Re: sandman420]
    #27308540 - 05/14/21 02:46 PM (3 years, 8 months ago)

Quote:

sandman420 said:
How big of a batch of agar do you make at one time? (ml)

A possible explanation could be that less genetics per transfer equals less dense growth.

Or maybe you messed up your agar recipe last round and that made a lot of weak plates possibly?


I mean both of those plates look just fine to me. But you said you had a whole batch of whispy ones so...




I usually do 250ml. (250ml water/12g malt extract agar)

What kind of mess up would contribute to weaker plates? Is extra agar powder worse than less?

Extras: Filter Print Post Top
OfflineJosborne
Stranger
Registered: 11/18/20
Posts: 184
Last seen: 3 years, 4 months
Re: Why agar transfers getting weaker? [Re: smalltalk_canceled]
    #27308543 - 05/14/21 02:48 PM (3 years, 8 months ago)

Quote:

smalltalk_canceled said:
Why is the bottom plate not good? Weak growth what




It's not as uniform/dense looking as my previous plates. The 9-12 o clock area looks a lot weaker than the rest of the plate. But I might be totally over thinking it haha. I was just surprised at the difference because my plates thus far have been pretty consistent.

Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,387
Trusted Cultivator
Re: Why agar transfers getting weaker? [Re: Josborne]
    #27308556 - 05/14/21 02:58 PM (3 years, 8 months ago)

When you do an extra small batch of agar that means that any mistakes or errors in the recipe are multiplied.

250 agar recipe: 5g agar 5g malt. 6 grams is 20% overshot. 7 grams is 40% overshot. 3 grams is 40% undershot. You get what I'm saying? How accurate is your scale even? I really recommend to make bigger batches, at least 500ml.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Edited by sandman420 (05/14/21 02:59 PM)

Extras: Filter Print Post Top
OfflineJosborne
Stranger
Registered: 11/18/20
Posts: 184
Last seen: 3 years, 4 months
Re: Why agar transfers getting weaker? [Re: sandman420]
    #27308570 - 05/14/21 03:11 PM (3 years, 8 months ago)

Quote:

sandman420 said:
When you do an extra small batch of agar that means that any mistakes or errors in the recipe are multiplied.

250 agar recipe: 5g agar 5g malt. 6 grams is 20% overshot. 7 grams is 40% overshot. 3 grams is 40% undershot. You get what I'm saying? How accurate is your scale even? I really recommend to make bigger batches, at least 500ml.




Oh wow. I never even thought of that. Thanks for pointing that out! :takingnotes:

And it's a pretty cheap scale so I'm assuming not crazy accurate. I will take your advice.

I originally was doing 500ML but found I always had a lot of extra left over unless I did at least 30 plates at one time—which it sounds like I might have to start doing!

I just hate wrapping plates. If I don't transfer during that session then it means I'll have to parafilm twice (once after solidified, then again next time I open it for a transfer). However I saw that some people just wrap the petri dish bag over a stack of plates until they decide to use them so I might do that...

Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,387
Trusted Cultivator
Re: Why agar transfers getting weaker? [Re: Josborne]
    #27308589 - 05/14/21 03:24 PM (3 years, 7 months ago)

I put them in stacks in large 2 gallon ziplocs straight in the hood, but I don't have sleeves because glass petris.

When I used sleeves I would take a few out so I could tuck the bottom all snug because I'm a pervert. Put those in another ziplock or suomething.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Extras: Filter Print Post Top
OfflineShakedown Street
Friend
Male

Registered: 09/05/20
Posts: 404
Loc: Well, well, well, you can neve...
Last seen: 1 day, 7 hours
Re: Why agar transfers getting weaker? [Re: sandman420]
    #27309081 - 05/14/21 09:40 PM (3 years, 7 months ago)

During winter past, I got good at making agar.    So I decided to have a speed race.    I made an inventory of all the possible spore prints, petri dishes of agar, and jars of LC I had that might possibly grow mycelium.    Then I made that number of agar petri dishes and  inoculated each and every one, marking each petri dish carefully to denote its source.

My only goal was to determine which agar made it to the edge of the petri dish first, #1,and which came in second place, #2.

Thereafter, I only transferred winners #1 and #2  to grain.    My reasoning was that I only wanted the mycelium that most quickly colonized agar because that mycelium would also most likely colonize grain the quickest, and avoid contamination.

My speed race was just a farmer's version of Darwin:    save the best, trash the rest.

Remember all life depends on water, and microbes need it too.  The faster the mycelium sucks it all up, the less likely some stray spore or bacterium can grow.    That was my reasoning.

Another positive thing that came from this speed race was that I easily trashed a whole shitload of losers.

After doing this I learned G2G transfers, a technique that allows you to turn one fully-colonized grain jar into ten fully colonized grain jars in 10 to 14 days.    Thanks to PhillyGoldenTeacher.

Because of this, I have many, many fully colonized grain jars.  Only one or two came out duds--that is, only a single clump  mycelium growing in them.    Straight to the compost heap they went.

I recall your darkness when it crackled like a thundercloud.

Edited by Shakedown Street (05/14/21 09:47 PM)

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* agar transfers buggers 4,656 12 11/03/07 10:44 AM
by orchidfanatic
* agar transfer substitution? omniscientlore 1,516 3 12/19/22 07:14 AM
by Screwup
* How to make agar 2 agar transfer? esp 6,944 7 01/08/04 11:08 AM
by YidakiMan
* agar to agar transfer ohmatic 16,335 11 01/12/21 09:00 PM
by bodhisatta
* agar transfers *DELETED* thisone 1,269 3 11/20/03 08:58 PM
by Mycomancer
* agar to agar / g2g degeneration effects ohmatic 2,744 11 01/17/05 03:46 PM
by MushroomFriend
* agar with malted barley syrup Hongosmeester 7,456 17 10/27/19 10:42 PM
by ABCMORPHS
* Very Nice New Recipe for Substrate bluhoney 2,586 8 05/26/05 10:27 PM
by bluhoney

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
317 topic views. 34 members, 120 guests and 21 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.007 seconds on 12 queries.