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OfflineLogicaL ChaosM
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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27096909 - 12/18/20 11:49 PM (3 years, 1 month ago)

Very cool man :awesome::thumbup:

I gotta try that next round :yesnod:


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: LogicaL Chaos]
    #27140119 - 01/10/21 04:11 PM (3 years, 1 month ago)

So I’ve got the PE/pfrs RADS (rub and double swab) in fruiting chambers.  16qt tub, 2 qts of rye spawn to CV.. The qts were inoculated with GLC made with the RADS SS.  It’s been 4 days



The last time I did this^^^ with pea+, I got an entire bin of cross fruits, so here’s to hoping :cheers:


Here’s the coir cakes I made with the RADS SS.. it was the first time attempting coir cakes and I made them too wet.. I ended up having to cut off some uncolonized areas at the bottoms of the cakes so hopefully I went far enough and they don’t tam out.. I’m hopeful though.  They’ve also been in the FC for 4 days



So hopefully in the next couple weeks I’ll know if I got my coveted red spore PE variety. :grin:

Today I put my pea+ f1/pfrs RADS brf cakes to a FC. I figured the best possible thing that could happen trying to cross the f1 pea+ with pfrs is, I could end up with a red spore pea+, a red spore AA+, and a red spore PE and the worst thing that could happen is I end up with an F2 pea+.. seems like a win-win to me, so I said fack it, let’s try lol



Those cakes colonized great and look awesome.. they reminded me of the first pea+ RADS SS cakes I made and got crosses at almost every inoc point, so I’m pretty excited about this grow.  The qts I made with the glc of that RADS SS we’re shook yesterday and they looked great pre-shake



When I made the glc grain jars for those^^^ qts, I made two 4oz rye jars... one I used to inoculate those qts and the other I spawned to CV in a little glad container and it’s probably a fewish days away from knots atm..


That’s why I like those little testers.. they give me a glimpse of what’s to be in a couple weeks

I Spawned these pea+ F2 qts to CV in a 16qt today too.. these were the qts I went spore print straight to grain that I used the in-jar printing method from a couple posts ago



Lots of fun and exciting possibilities coming up in the next couple weeks!  Really need the mush gods to shine on me :lol:

Faht


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27177326 - 01/30/21 02:30 AM (3 years, 17 days ago)

Why do you like the print on the bottom of the jar if your making swabs anyway.


Edited by All41 (01/30/21 02:34 AM)


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: All41] * 1
    #27177332 - 01/30/21 02:44 AM (3 years, 17 days ago)

So I can come back to the print and grab spores from that gen later and it’s cleaner than a swab.  Swabs are easy to make.. takes like two minutes.  The swabs are mainly just back up.  The printing in a jar is whole process that I use an oven bag that I pc and is kind of a PITA compared to swabbing but I trust it more to go straight to grain with.

Kind of forgot about this thread lol.. I have results from all those tubs..

the pea+ F2


The PE/pfrs cakes


Got a bunch of crosses on the cakes.  Purple spore dropping/heavy PE pheno fruits.  The purple dropping spores is a dead giveaway for a cross


This guy I printed (redspores) and I believe is a cross based on color/cap shape/stem bumps.  They’re already germinating on rye


Pe/pfrs 16qt bin.. I believe there’s lots of crosses going on in here.. no reason to think differently based on past experience with the cakes above and how it worked with pea+ and the pea+ f1/pfrs bin.. the PE is showing through on a lot of fruits.  I cloned a couple


Pea+ f1/pfrs produced normal looking fruits that were super fast (14 days spawn to harvest) and didn’t look like PE, AA+ or pfrs.  They produced purple spores but the cap shape isn’t like any of those 3... totally not what I was expecting lol. I took swabs of these but I’m not going to pursue these further for now.. this was just for fun to see what would happen if I crossed an f1 fruit with something


For reference, this is what pea+ looks like fruiting (we all know what PE and AA+ look like)


The next cross I’m going to be working on is PE/crs.  Making some qts tomorrow.  I will be moving forward with pea+ and the PE/pfrs.  Here’s 1 of the 2 pea+ F2 fruit prints I’m going forward with


Faht


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InvisibleQM33
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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27307989 - 05/14/21 07:46 AM (2 years, 8 months ago)

Sick deal man. I was thinking of doing something similar, especially  after reading josex double dip idea.
Thank you!


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: QM33]
    #27308085 - 05/14/21 09:09 AM (2 years, 8 months ago)

I’ve got to update this thread lol. I’ve done this successfully a bunch more times

Faht


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InvisibleQM33
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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27308088 - 05/14/21 09:11 AM (2 years, 8 months ago)

I'm going to be honest man, the OP is pretty intimidating for the tek, allot of info there

But thank you so much <3


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: QM33]
    #27308119 - 05/14/21 09:31 AM (2 years, 8 months ago)

You can get rid of all the intimidating info and keep it really simple.. take two swabs... sterilize two jars of water... dip the swabs in the first one a few times... go to the second jar... put the swabs in the water and rub them together where the spores are until they’re free of spores... suck up the water with the spores in it.  Done.

If you do it in front of a hood, that’s the best.  You can do it in a SAB or you can get crazy like me and do it in an oven bag.  The results are the same.  :thumbup:

Faht


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OfflinekingboomerDiscord
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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27371204 - 07/02/21 02:46 AM (2 years, 7 months ago)

How would one go about this with 2 caps?  Smaller the better im thinking?  Sorry if this is OT...


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: QM33]
    #27371378 - 07/02/21 08:15 AM (2 years, 7 months ago)

What do you mean exactly?  You can use any size cap to swab.. I prefer bigger ones since the gills tend to be spaced farther apart :thumbup:  or maybe you’re referring to something else?

Quote:

QM33 said:
I'm going to be honest man, the OP is pretty intimidating for the tek, allot of info there

But thank you so much <3




Oh shit.. didn’t realize we had conversed in this thread and here I am linking you to it :facepalm:  I’m getting old, man.. can’t even remember a month and a half ago :lol:  my B

Faht


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InvisibleQM33
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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27371391 - 07/02/21 08:28 AM (2 years, 7 months ago)

Ha lul. No worries bro, I'm sure you have alot of important shit to keep in your brain.


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: QM33]
    #27458443 - 09/06/21 04:05 PM (2 years, 5 months ago)

Making a PE/crs F2 swab into spore solution and thought I’d document the process a lil better.  These f1’s didn’t drop spores so I had to swab


Here’s just an update to how I’m getting this shit done.. the H2o2 “portal” is newer since I made this thread

The set up


What’s pc’ed in the oven bag:
-4oz rye jar (this was already pc’d once for 2 hours so I got not have to pc it inside the oven bag for 45 minutes to sterilize everything else).  I could just inoculate this jar outside the bag but I’d rather not inoculate through an iso wiped SHIP
-2 jars of water.. one is to rinse the swabs and the other has 17ml of water (just enough to submerge the swab tips) and that’s the jar I rub the spores off in and suck up via syringe
-empty syringe
-SS scissors
-A plate to hold everything

I tape up the bag opening all but about ~3” on one side and then roll and fold that opening and then zip tie it and pc for 45 minutes.  I also make 2 CV/brf cakes that I pc along with the bag

After pc’ing, I blow the bag up a little via the syringe barrel poly filter and fish tank bubbler (takes about 5 minutes.. the filter is very restrictive)  Then I use a modified glad container filled with h2o2 as a way to get the swabs into the bag without exposing it to room air… the swabs are vacuum sealed (swabs and inside of vac pouch is iso’ed prior to sealing.  I break the swab sticks about half way up so I can bend swab pack to get it through the bag hole easily and so they’re more manageable inside the bag.

I cut the zip tie and unroll and unfold while it’s submerged in the H2o2.. the bag opening is kind of stuck together from pc’ing which is nice so there’s not a lot of chance for air to get in until I physically pull it apart when it’s submerged.

Then I stick one end of the swab pack into the bag opening under the H2o2 and pull it into the inside of the bag… then I zip tie the bag opening back up while it’s still submerged


Then I blow the bag up more so there’s plenty of room to work in

I use the scissors (very carefully as to not cut the oven bag) to cut open the swab pack just below the break in the sticks and pull the back off.  While I didn’t let any air inside the bag, I still don’t want to touch the swab tips to anything except the jars of water, so I leave them inside the vac pack until I screw the lid off the first rinse water jar. 


I remove the swab from the vac pack and dip them into the first jar of water.. in this jar, I rub together all the parts of the swab tips that don’t have spores on it and also gently rub once or twice where there are spores… the idea with this first jar is to rinse off any possible contams that may have landed on the swabs when swabbing the gills.  Then I set the swabs in that jar while I take the lid off the second spore water jar.


Then I vigorously rub the swabs together where the spores are until they’ve been dislodged into the 17ml of water… suck up 1ml (2ml if I’m inoculating 2 grain jars) into the syringe and inoculate the grain jar… then suck up 10ml into the syringe.  Cut the bag open and remove the syringe and grain jar.

Here’s a comparison of how well rubbing the swabs together dislodges the spores into the water.. this is after I have cut the bag open.. before:

After:


Then I cut off the reusable pieces from the bag and discard the rest.. then inoculate the cv/brf cakes


Here’s how the pea+ f5 grain jars (I shook the grain jars yesterday so they should be done in the next couple days) and cakes are doing from the swab to SS I did last week.. notice how well the rubbing declumped the spores from the swab tips


I’m actually swabbing the prints ppl send me so I can make these because it works so well

Faht


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OfflineA.k.aM
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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster] * 1
    #27458478 - 09/06/21 04:44 PM (2 years, 5 months ago)

Just gonna add this to my threads for later

:thumbup:


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: A.k.a]
    #27511988 - 10/21/21 02:40 AM (2 years, 3 months ago)

Thought I’d add to this what my next plan of attack is because I think it’s a good one and gonna make things real easy.. pretty simple.. instead of going through the trouble of making a syringe after I rub the swabs together in the second jar of water, I’m gonna just stick the swab tips into the grain jar and twist to get the spores into the grain. 

It worked when I basically streaked the swabs across the top of the grains when I made the Gandalf jars.. I’ve been making pf cakes along with the glc grain jars as back up but they’ve been unnecessary so might as well just cut out the extra step of making a syringe and just dip the swab tips directly into the 4oz grain jar (after rubbing them together in the second jar of sterile rinse water).  The swab tips are basically dripping with spore water after the rub and should have no problem transferring plenty into the grain directly.. the Gandalf baggie I made with the grains I directly swiped were clean enough to go to 2 flushes and get clean clone material:


I’m finding that the small amount of grain colonizes so fast that even a bit of bacteria doesn’t stop a baggie grow to get clone material and then it’s home free from there. 

I’ll update with how swabbing directly to grain goes (since I’ve only done it once but I don’t see how cutting out the syringe part would affect the outcomes that I’m already getting).. I’m gonna do pea+ f6 and yeti from swab next

Faht


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster] * 1
    #27512182 - 10/21/21 08:08 AM (2 years, 3 months ago)

I tried this the other day but what I did was take the plunger out and stick the barrel through a piece of cardboard to hold it up.


Then I got a second syringe full of sterile water, put the swab just inside the propped up barrel, and squirted it with the water. Once it was wet I stuck the syringe into the swab and kinda pulled it loose and then shot the rest of the water out from inside the cotton.


Then I pressed the swab against the side of the barrel to get the rest of the water out and put the plunger back in.


This stuff works great, I was really surprised the amount of spores that came off.


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: A.k.a]
    #27512256 - 10/21/21 09:39 AM (2 years, 3 months ago)

:threadmonitor:


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: Guerrilla]
    #27522287 - 10/28/21 11:52 PM (2 years, 3 months ago)

So I made 2 pea+ f5 and 2 papu F2 glc jars where I dipped the wet swab directly into the grain jars on 10/24 and so far the pea+ has germed. 


My plan is to leave one of each to pin invitro and clone a pin to another grain jar.. like a plate pin and the other other with be GLC’d to jars as long as they look decent.. all will be spawned to cv in a baggie once “used”

Gotta good feeling about these new steps

:popcorn:

Faht


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27528315 - 11/03/21 02:28 AM (2 years, 3 months ago)

The swab “dip” jars are looking good a couple days after first shake.. shook them again right after pic.. should finish up pretty quick after they recover


Faht


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27546229 - 11/16/21 07:32 PM (2 years, 2 months ago)

So far the dip has worked great.. fir the pea+ f6, both 4oz jars fully colonized and one was used to knock up 2 qts (shook them yesterday) and the other, I’m waiting to see invitro pinning to clone and for the papu f3, I used one of the jars for glc before it was fully colonized and looked really clean so I just tossed the grains from that one because they wouldn’t have survived being spawned but the other one I’m also waiting on invitro pins for cloning


The pea+ f6 glc grains were spawned to a baggie and colonized great so if the qts don’t work out, it looks like I’ll still get swabs to move onto f7, np


Once I get pin clones from each of the other jars, they’ll be spawned to baggies as well.. the papu qts looked great when I shook them so I think they’ll be a tub of them coming up but gotta love backup plans lol

Faht


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Re: Making spore syringes from swabs (I’m using an oven bag**optional) (moved) [Re: fahtster]
    #27547144 - 11/17/21 02:49 PM (2 years, 2 months ago)

Inspiring man
I've been getting swabs on tiny pf cakes by tearing bits off and burying them, then casing and fruiting for tissue samples. I don't think I'll bother trying to isolate on agar before fruiting again. The process moves much faster it's easier and have 15 or 20 projects at a time isn't a daunting task. If it pins invitro cause you couldn't get to it mission accomplished.


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