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Najxwiinymuuxy
Teonanacani

Registered: 03/30/21
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New Panaeolus Cambodginiensis grow, starting from agar 1
#27305058 - 05/12/21 12:32 AM (3 years, 8 months ago) |
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Hello dear fellows in the Mushroom. After the first and unfortunately unsuccesful trial with Panaeolus Goliath, I'm starting a new one, trying to apply the knowledge I got from the experience itself and the advice from dear kind fellow mushroomers.
This is just the beginning, the photos of the mycelium transfers (from previous agar plate) to agar plates; they seem to be growing really nice. The surgical procedure was started at this saturday evening, so they just have 3-4 days, and are growing at a high velocity. As you can see they seem very healthy, the only thing that worries me is excess of moisture.. I don't know if it is because I used this wrapping kitchen plastic and micropore instead of parafilm for sealing the jars, cause I ran out of parafilm.
They are growing in an agar rich in malt and very fine horse manure, to which I also added 5 ml of peroxide to prevent bacteria and spores from developing (I know, I know this kind of techniques are very criticized by many, but I was really worried about bacteria that had just eaten my poor previous growings), and the mycelium has been growing really fast and healthy, all of the dishes are clean and uncontaminated and mycelium looks strong and agressive. They are incubating at 26° C (I raised a little the temperature, so they grow faster, but not so much that they get dry or overdevelop, and will try to keep this temperature with the jars, then lower it a bit after casing, and then lower it more for fruiting... What do you think?).
So here are the first pictures, and will be updating often so fellows in mushroom can see the process and developing. I'm very happy to share with you, as noob as I am, but I know many great growers here can appreciate and all your sugestions and comments, observations are really welcome! (and needed, I do want to improve for real, and I know I'm in the right place).
Soon I want to inoculate 16 450 ml jars using 4 agar dishes, pouring a quarter of the dish per jar, also try to elaborate a liquid culture with sterile water with malt and karo and some agar mycelium wedges.
Thanks a lot to everyone for taking the time, and I'm very excited to share this whole process.
¡Blessings!





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tiptrippy
The Mechanic



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Re: New Panaeolus Cambodginiensis grow, starting from agar [Re: Najxwiinymuuxy]
#27305061 - 05/12/21 12:39 AM (3 years, 8 months ago) |
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give.more
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Re: New Panaeolus Cambodginiensis grow, starting from agar [Re: tiptrippy]
#27305075 - 05/12/21 01:19 AM (3 years, 8 months ago) |
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I just harvested from my first pan cambo flush, I'm excited to follow along.
-------------------- Hi, I'm Dav. It was David but I lost my ID.
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Regis
Haben wir noch pepps?

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Re: New Panaeolus Cambodginiensis grow, starting from agar [Re: give.more]
#27305106 - 05/12/21 02:12 AM (3 years, 8 months ago) |
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Good luck mate
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Najxwiinymuuxy
Teonanacani

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Re: New Panaeolus Cambodginiensis grow, starting from agar [Re: Regis]
#27306054 - 05/12/21 05:03 PM (3 years, 8 months ago) |
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Thanks mate!
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Najxwiinymuuxy
Teonanacani

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Re: New Panaeolus Cambodginiensis grow, starting from agar [Re: give.more]
#27306057 - 05/12/21 05:05 PM (3 years, 8 months ago) |
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Great! It is great to be sharing with you fellows! Would be interesting to see your harvest too! I'll be updating often!
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Najxwiinymuuxy
Teonanacani

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Re: New Panaeolus Cambodginiensis grow, starting from agar [Re: tiptrippy]
#27306060 - 05/12/21 05:07 PM (3 years, 8 months ago) |
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Hey Trippy! Nice to see ya here! Will be awesome to have your observations! I'll be updating!
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Najxwiinymuuxy
Teonanacani

Registered: 03/30/21
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Re: New Panaeolus Cambodginiensis grow, starting from agar [Re: Najxwiinymuuxy]
#27306155 - 05/12/21 06:26 PM (3 years, 8 months ago) |
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12/05/2021 Here are the photos of my Petri dishes of today, in just one day they almost doubled their size! I think tomorrow will be a good day to start the spawning on grain jars, and also try an agar wedge based Liquid Culture.
Although I'm still wondering if this little friends are Panaeolus Cambodginiensis Goliath or Thai Panaeolus/Copelandia Cyanescens Suphanburi Goliath... I'm still not sure, as we yet have to wait for the mushies to fruit. The thing is that the bros who gave me the LC told me these were "Hawaiian Copelandia Goliaths".. hmh.. For what I know there is not such thing as Hawaiian Copelandia Goliaths, so they must be either cambodginiensis goliaths or thai cyanescens goliaths (or hawaiian panaeolus cyanescens)... but who knows, maybe you have heard about those "Hawaiian Goliaths". I don't think I've been scammed as the mycelium looks totally panaeolus and these people have good batches of mushrooms, so there must have been a confusion on the naming, they sent me this liquid culture and a spore print of Mexican Huaxtecan Cops/Pans Cyanescens var. Zapotalillo, Veracruz. So lets wait and see!!
I have a question, do you think 26 °C / 78.8 °F would be a good temperature for growing the spawn? I know some of you don't put artificial heating at all, but I want them to colonize a little faster, although don't want to burn them or make them overgrown, or succumb to bacteria, as this already happened to me when incubating at 28-28.5 °C / 82.5-83.3 °F. Of course there won't be a heating pad transmiting heat directly to the jars, they will be separated from the heat source, which is controled by a thermostat and heat sources are set in a low intensity level.






So lets see my friends! Will update soon, when they start colonizing grain. Blessings!
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tiptrippy
The Mechanic



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Re: New Panaeolus Cambodginiensis grow, starting from agar [Re: Najxwiinymuuxy]
#27306326 - 05/12/21 08:37 PM (3 years, 8 months ago) |
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Looking good Naj! If you choose to use these plates to inoculate grain I would personally probably only use the plate from the first picture. You can of course try any of them you wish but don't be afraid to toss a jar out if it starts to turn bacterial.
78F is a perfect temperature for your spawn to colonize the grain. No need to supplement the heat source, especially with a fast grown species like Panaeolus.
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Najxwiinymuuxy
Teonanacani

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Re: New Panaeolus Cambodginiensis grow, starting from agar [Re: tiptrippy]
#27306402 - 05/12/21 10:19 PM (3 years, 8 months ago) |
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Thank you Trippy! I'm excited with this new grow, and hope I can achieve good results now, perhaps not as good as your pans hehe as those are incredible, but at least some good mushies to start cloning.
I want to ask you, what it is what makes you think that only the plate from the first picture should be used to inoculate grain? I mean what it is that tells you that, from your expertise, like what features, or what should I look for in a panaeolus agar culture to know it is the best option? Maybe I should reproduce more plates with that one to obtain similar growings then, but what it is that distinguishes it?
Did you saw some signs of bacterial contamination already present in some of these plates? I know some look like with blueish elements, but that is just the marker I used in the plate, that was very thin and inefficient.
And sure, I will toss immediately every jar I see has the slightest sign of contamination this time!
I thought about inoculating each of the 450 ml jars with a quarter of these full mycelium circles, each quarter cut into four or three parts also, to have more contact points in the grain jars, but I don't know if that might be excesive and just a tiny piece would be sufficient?
About temperature, I don't think we will reach the 78F naturally here, at least not yet, what do you think? Still would be better for grain to colonize in natural temperature? Now we have here 71.6 F in the highest end, and 53.6 F in the lowest.
I hope everything goes well.
Thanks a lot bro!
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tiptrippy
The Mechanic



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Re: New Panaeolus Cambodginiensis grow, starting from agar [Re: Najxwiinymuuxy]
#27306419 - 05/12/21 10:52 PM (3 years, 8 months ago) |
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#1 agar plate looks the most uniform and healthy. #2 has a weird spot at 4:30-5:00 that doesn't look uniform. It might just be the picture or the plastic wrap but it's hard to tell from here. #3 has that whole section on the right side of the plate which makes it look like some other organism is growing there #4 has sections on both the left and right sides that also look like #3 #5 looks like it might be a 2nd picture of plate #3 with different lighting #6 looks like it would be a good 2nd choice. It looks uniform and clean
Regarding temperatures. Usually if you feel comfortable in the room then so will your mycelium. If you want to use a higher temp then it's best to use a small space heater to heat the ambient air rather than a heating mat or some type of incubation tub as they have a tendency to dry the grains out.
And yes it would be ok to use a quarter of the dish per jar and yes it's ok to cut that quarter into smaller pieces for more inoculation points. Just be mindful of your sterile technique
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Najxwiinymuuxy
Teonanacani

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Re: New Panaeolus Cambodginiensis grow, starting from agar [Re: tiptrippy]
#27307537 - 05/13/21 09:32 PM (3 years, 8 months ago) |
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Great! thanks a lot for the feedback and helping me learn, Trippy, I know I may ask what seems very foolish or naive questions, but there's a lot of informaction on the internet that contradicts one another and it is great to have this advices from you, an expert grower of Panaeolus himself, you have been very kind.
Great detailed analysis of those plates! Thank you! I won't use for spawning into grain the ones that you signaled as suspicious, I can see why now. Although the ones with different growing, that you said seemed like 2 different organisms in the plate are actually like that because of the timing on pouring agar and that there was a decline in the surface where I had the SAB, so the surface of agar didn't get even; so I think it doens't have contamination, but anyway, I wont use parts from the smaller or decline parts, that have a weaker growing.
Thanks again for the great tips and observation. I'll begin PCing my grain in a few moments for 2 huors... I'll be posting more soon.
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Najxwiinymuuxy
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New Panaeolus Cambodginiensis grow (update, colonizing jars) [Re: Najxwiinymuuxy]
#27315176 - 05/19/21 02:05 PM (3 years, 7 months ago) |
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Hello dear fellow mushroomers.
I'm updaating with (what I hope to be) good news.
Today, after 5-6 days of inoculation, incubating at a temp range of 24.07 °C / 75.326 °F to 25.8 °C / 78.44 °F (I'm trying to reduce as much temperature so there won't be overgrown or contamination, I hope this is enough, as some of these myceliums look veryvdense and cottony-fluffy already).
They look quite well and healthy, mycelium is cottony and dense but seems uncontaminated in every jar (16 in total, I'll show you some in the pics, some more I won't upload the photos as are in similar situations).
And well, today I've just give them a shake, and from white (many jars were already all white, and somne still with not very dense growing, more "webby" and light like), all grains are grain-colored again. I'm worried I screwed it up though.. As mycelium was already coverin all the visible grain, thick and dense, what seems healthy, in most jars, and in some jars "like trying to get the hell outta the jar", to quote a fellow from a video.
I hope I didn't ruined it (it colonized super fast, because I put a sliced quarter of big Petri dish mycelium in agar per 500 ml. jar), maybe they had already colonized enough, but as I have this idea one does need to shake jars at least once, even if everything is colonized, but 24-48 hours prior to mixing with bulk... Please help with you expertise and knowledge, your opinions, etc., my friends.
I'm also starting to worry about moisture in jars, as some seem (not totally, but) a little dry.
Gh God I hope I didnt screw it! I'll show you the photos before shaking, friends, so you can see by yourselves (I didn't post after shaking, because it pretty much looks like normal grain).
A 
B 
C 
D 
E 
F 
G 
H 
I 
J 
K 
L 
M 
N 
Ñ 
O 
Blessings.
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Najxwiinymuuxy
Teonanacani

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Re: New Panaeolus Cambodginiensis grow (update, colonizing jars) [Re: Najxwiinymuuxy]
#27315177 - 05/19/21 02:06 PM (3 years, 7 months ago) |
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Should I wait until all the visible grain is colonized by mycelium again?
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tiptrippy
The Mechanic



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Re: New Panaeolus Cambodginiensis grow (update, colonizing jars) [Re: Najxwiinymuuxy]
#27315410 - 05/19/21 06:11 PM (3 years, 7 months ago) |
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Those are all looking pretty good to me.
A, B, I, and M look like they are not 100% colonized yet.
Yes you want to wait for them to recover back to 100% before you spawn them to make sure they are still healthy with no hidden contams.
What is your plan for spawning and fruiting?
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Najxwiinymuuxy
Teonanacani

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Re: New Panaeolus Cambodginiensis grow (update, colonizing jars) [Re: tiptrippy]
#27315747 - 05/20/21 12:41 AM (3 years, 7 months ago) |
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Hi Tiptrippy! Nice to see ya here! Always the good vibes and wise advice.
You're right, those ones aren't fully colonized, anyway, I hope everything goes smooth and all or most jars achieve a full clean grow, as I'm a little wary of overgrow or overleap (which should be the most correct term?).
I will check them again in 2-3 days hoping they're colonizing or have colonized again. Some rye grain seem dry, but they kinda look like those floating grains I was trying to separate by water and collander.
My plan for spawning, as soon as jars are ready, is to pasteurize some straw-manure-vermiculite mix (like twice the volume of grain) and mix it with the colonized grain. Then incubate them in tub Inside tub, at the same temperature, maybe without added water or perlite yet, with a lid, wait some days for grain to colonize again and spawn it, then lower temperature, wait till the mycelium emerges from casing and put them to fruit in a chamber tub in tub, with perlite, water, air pumps with some bubble stones, at a lower temperature, and two fans, one at the upper part, to get air out, one at the other side, lower part to draw fresh air, and some holes, all holes and fans with polypropylene filters, seting everything with thermostat and timer for fanning. Well, that's my plan ideally.. I'm very idealist maybe, but attentive to learn and keep on learning from the experimented fellow growers and practice too! ... hah I hope I can get some mushies and have even little but some finished product at least
Also I wonder if I could use the bulk prepared like 2 weeks ago, just pasteurize it again, it doesnt smells or looks bad, has been in fresh place with a lid, the straw was pasteurized before heat pasteurization with hydrated lyme, and the manure is pretty vine-clean one, would it be ok, or I better prepare more new bulk?
If you have any suggestions, everything would be very welcome and thanked!
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tiptrippy
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Re: New Panaeolus Cambodginiensis grow (update, colonizing jars) [Re: Najxwiinymuuxy]
#27315776 - 05/20/21 01:27 AM (3 years, 7 months ago) |
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You are concerned about overlay. As long as you don't case the trays too early you shouldn't have to worry about overlay. After you spawn the jars to bulk you want to wait 24-36 hours after you see 100% colonization before you add the casing layer.
Your fruiting setup sounds like it should work ok.
I'm honestly not really sure if it is ok to use the bulk you have already pasteurized if it has been sitting for 2 weeks. I always use my substrate within 1 week. It might work ok. To be safe you can use just one of the spawn jars with the old sub and use the rest of the spawn with fresh sub.
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Najxwiinymuuxy
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New update on Panaeolus Cambodginiensis grow (fructification process) [Re: tiptrippy]
#27332829 - 06/02/21 03:43 PM (3 years, 7 months ago) |
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Hello dear fellow mushroomers, Trippy, hope you've been all good.
I took some days to update, but since the last post there has happened quite some things. By May 22th I spawned my 22 jars (minus 2 that I suspected there was probably contamination) over 2 large trays, in a 60% straw 30% manure and the rest vermiculite and also hydrated lime, in which I also prepasteurized the straw a day prior (then, the day I spawned, I pasteurized by heat)... So far, everything seemed nice, all the sub was quickly colonized (at least what I can see). After a couple of days I then cased with a mix of 80% peat moss, 10 vermiculite and 8-10 hydrated lime, all pasteurized. In some days the mycelium had colonized most of the casing (which according to what I've read, is not quite allright for mycelium to eat the casing.. correct me if I'm wrong or there is an exception for this species).
This is the spawn before and after casing:
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2 
3 
4 
5 
Then, after a few days, most of the casing seemed to be colonized by mycelium, so I added another thin casing layer, mostly peat moss treated with a little hydrated lyme, but this time with more vermiculite, hoping this would help to keep moisture and that it won't be eaten by mycelium and it would fructify soon... After a few days this is the result again:
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7 
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I have the trays inside a tub with three holes on each long side, covered with polypropylene filters, one fan that draws fresh air inside, at a low height and another fan at the upper part (at the sides of the tub) that draws air out, both fans are activated by a timer for 5 minutes 6 times a day. The tubs where the trays are, are also filled with some water and there is an air pump connected to 2 hoses that go to each side, connected to air stones that provide also fresh air and bubbles all day. The tub-tray that is in the lower part seems very humid all of the time (I need and hygrometer, as the ones I had don't work anymore), but the tub and tray that are at more height dries more frecuently and I need to sprinkle 2-3 times a day the inside of the big tub.
The thing is that I just see the mycelium which looks strong and healthy (and all the room smells nice, like forest-freshy-mushy), colonize again and again but I don't see signs of fructification, even after there have passed almost 5 days since the last casing... I wonder now if I'm doing something wrong, if there is not enough moisture (which I don't know how to increase as I spray water and the trays themselves are in water) or appart from the automated fans they may need manual air fanning?? Also the temperature is on its own, now stabilized from 24.5 to 25.6 at most, which I believe are good temps for fruiting...
So I don't really know if I just need to wait or what should I do. Until now everything seemed to have been succesful, but it seems mycelium cannot fructify, I don't see any signs of pinning, just thick cottony mycelium... or maybe I should just wait more?
Well, as always there has been a pleasure to share, and I really hope I have success and can make it nice till the end of harvest and share it with you. I'm attentive to your advices as always.
Blessings.
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Najxwiinymuuxy
Teonanacani

Registered: 03/30/21
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My Fruiting Chambers for Panaeolus Cyanescens/Cambodginiensis [Re: Najxwiinymuuxy]
#27332963 - 06/02/21 05:47 PM (3 years, 7 months ago) |
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By the way, I share some photos of my fruiting chambers, that, as I described earlier, consist on a tub in tub or tray in tub, with some water and two hoses on each side of each one, conected to a water pump and two small diffuser stones. Each one has a pc fan on each side, one at lower height, to draw fresh air inside and the other at upper height, to draw air outside (they have polypropylene filters, with the ones on the lower fans fully taped and closed and the upper ones that draw air outside with a little tear on the filters, as if I completely close the air mostly bumps).
They have also temperature controlers connected to water heaters, but I'm not using those, since is getting very hot here and I think mushrooms are at optimal fruiting temperature... 76-77 F / 24.5-25 C.
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What do you think?
I have cased 2 times, and have passed 5 days since the last casing, which have been partly eaten by mycelium and I don't see pinnings still.. I wonder if I should just wait more or I'm doing something wrong?.. There are more details provided at the previous post.
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macsone41
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Re: New Panaeolus Cambodginiensis grow, starting from agar [Re: Najxwiinymuuxy]
#28388588 - 07/07/23 04:39 PM (1 year, 6 months ago) |
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Looks great
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