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OfflineFryingmantis
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Is this cobweb mold?
    #27304804 - 05/11/21 07:35 PM (3 years, 8 months ago)

So sad to see this if it is. I was excited about the growth in the last picture, then I noticed the black line...

Is it okay to have condensation in the jars? the ones that I'm suspicious of having contam's seem to have condensation.

Also, would it make any sense that a B+ MS jar is growing much quicker than a golden teacher MS jar? Could it be because its sitting in a spot that's at about 80 degrees as opposed to the GT sitting at 75? Or is quicker growth likely due to contam?

Any advice? =/
I have a ton of jars inoculating, I hope most of them are okay at least

I think if I got contams, its because I used micropore tape for air filter, and part of the adhesive bit came off leaving a slight opening on a couple of the jars for a couple hours until sealing it back up after Pressure cook..

Somebody suggested to put a layer of the tape upside down, then another layer right side up to make the filter and not let any grains stick to it, but following that advice sort of screwed me up I think.


Edited by Fryingmantis (05/11/21 07:48 PM)

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InvisibleLadysKnight
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Re: Is this cobweb mold? [Re: Fryingmantis]
    #27304822 - 05/11/21 07:55 PM (3 years, 8 months ago)

None look good, the the one in the middle easiest to see, gray mold in the middle of jar.

As someone mentioned earlier, use agar.


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InvisibleFeasoghorm

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Re: Is this cobweb mold? [Re: Fryingmantis]
    #27304828 - 05/11/21 08:00 PM (3 years, 8 months ago)

The 3rd pic doesn't look too bad but the other two look fucked. Got two different things growing in the jar in the 2nd pic. That's odd growth in the first one. And they all way too wet.

Get your grain much drier b4 jarring (i dry with a fan on high for two hrs) and pc for 2hrs. Make sure you got your pc procedure down pat.

After.that we need to go over what you doing when you inoculate.

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OfflineFryingmantis
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Re: Is this cobweb mold? [Re: Feasoghorm]
    #27304864 - 05/11/21 08:24 PM (3 years, 8 months ago)

Quote:

Feasoghorm said:
The 3rd pic doesn't look too bad but the other two look fucked. Got two different things growing in the jar in the 2nd pic. That's odd growth in the first one. And they all way too wet.

Get your grain much drier b4 jarring (i dry with a fan on high for two hrs) and pc for 2hrs. Make sure you got your pc procedure down pat.

After.that we need to go over what you doing when you inoculate.




Is it okay to leave the oats to dry overnight on a mesh net on top of a towel, then load up and pressure cook the next morning?

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InvisibleFeasoghorm

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Re: Is this cobweb mold? [Re: Fryingmantis]
    #27304889 - 05/11/21 08:41 PM (3 years, 8 months ago)

Sure mang, give that a try.
Grain pisses out moisture during a PC cycle then reabsorbs in the subsequent days. If you get it to where it all reabsorbs, then your within range.
Ive had batches so dry they pissed out NO moisture at all and mycelium still grew fine on it.

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Invisiblepablokabute
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Re: Is this cobweb mold? [Re: Fryingmantis]
    #27304910 - 05/11/21 08:52 PM (3 years, 8 months ago)

Quote:

Fryingmantis said:
Quote:

Feasoghorm said:
The 3rd pic doesn't look too bad but the other two look fucked. Got two different things growing in the jar in the 2nd pic. That's odd growth in the first one. And they all way too wet.

Get your grain much drier b4 jarring (i dry with a fan on high for two hrs) and pc for 2hrs. Make sure you got your pc procedure down pat.

After.that we need to go over what you doing when you inoculate.




Is it okay to leave the oats to dry overnight on a mesh net on top of a towel, then load up and pressure cook the next morning?





Yes. Thats what I do sometimes when I pass out in the middle of work. Heh


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OfflineFryingmantis
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Re: Is this cobweb mold? [Re: pablokabute]
    #27304959 - 05/11/21 09:51 PM (3 years, 8 months ago)

Thanks for the feedback guys.

As for my grain jars,

what would you guys do if you wanted to get things moving as quickly and sustainably as possible?

I think I may have fucked up 3 entire MS syringes.

I have 8 jars that seem to be healthy that I hope to spawn to bulk soon. Maybe next week.

And I have 1 full syringe + one with 1 ML.
I'm thinking the best move forward is to G2G the healthy jars before spawning to bulk, and in the meantime use the MS I have on agar, then transfer the agar to grain from there. One question I have.. With agar,
one petri dish can technically transfer into multiple jars right?

and the same principle with G2G? Does that mean that I could theoretically inoculate 20 more jars with one fully colonized one?

Thanks again for all the feedback. Sorry for all the mess of questions, I'm just trying to orchestrate the best way forward after finding out I wasted almost all my MS

Edited by Fryingmantis (05/11/21 09:52 PM)

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InvisibleFeasoghorm

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Re: Is this cobweb mold? [Re: Fryingmantis]
    #27304968 - 05/11/21 09:58 PM (3 years, 8 months ago)

You cud technically inoculate several jars with samples cut from a single plate. Gonna take quite a while to colonize.
A single jar of clean spawn can technically inoculate as many jars as there are colonized grains in the master jar. More colonized grains means more inoculation points equals quicker colonization, in theory.

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OfflineFryingmantis
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Re: Is this cobweb mold? [Re: Feasoghorm]
    #27304980 - 05/11/21 10:14 PM (3 years, 8 months ago)

Okay right on. If the MS I have is from a trusted/ sponsored vendor, odds are that its clean right? So I'm thinking I'll use 8 ml on 8 more jars with more sterile procedure this time,
and then ill use the remaining 3 ML for Agar.

1 ML of MS solution per agar dish is fine right?

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InvisibleFeasoghorm

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Re: Is this cobweb mold? [Re: Fryingmantis]
    #27304996 - 05/11/21 10:42 PM (3 years, 8 months ago)

I wudnt assume that any syringe is free of contaminants until ive tested it on agar. Because of this i advise against MS to grain, but do as you wish.

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OfflineFryingmantis
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Re: Is this cobweb mold? [Re: Feasoghorm]
    #27305004 - 05/11/21 10:55 PM (3 years, 8 months ago)

Quote:

Feasoghorm said:
I wudnt assume that any syringe is free of contaminants until ive tested it on agar. Because of this i advise against MS to grain, but do as you wish.




Alright fair enough. I'll just do G2G, and save all the MS for agar
thanks

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OfflinePBJ710
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Re: Is this cobweb mold? [Re: Fryingmantis]
    #27305036 - 05/11/21 11:46 PM (3 years, 8 months ago)

Quote:

Fryingmantis said:
what would you guys do if you wanted to get things moving as quickly and sustainably as possible?




Sustainable = clean cultures.  Theres really no way around it.  Get started on agar and clean up your cultures.

Quote:

Fryingmantis said:
Okay right on. If the MS I have is from a trusted/ sponsored vendor, odds are that its clean right? So I'm thinking I'll use 8 ml on 8 more jars with more sterile procedure this time,
and then ill use the remaining 3 ML for Agar.

1 ML of MS solution per agar dish is fine right?




1 drop of MSS on a loop that is streaked across the plate is ideal.  1ml is WAY too much and will increase the likelyhood of growing contams on the plate.  Never assume anything is clean.

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OfflineFryingmantis
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Re: Is this cobweb mold? [Re: PBJ710]
    #27305049 - 05/12/21 12:08 AM (3 years, 8 months ago)

Quote:

PBJ710 said:
Quote:

Fryingmantis said:
what would you guys do if you wanted to get things moving as quickly and sustainably as possible?




Sustainable = clean cultures.  Theres really no way around it.  Get started on agar and clean up your cultures.

Quote:

Fryingmantis said:
Okay right on. If the MS I have is from a trusted/ sponsored vendor, odds are that its clean right? So I'm thinking I'll use 8 ml on 8 more jars with more sterile procedure this time,
and then ill use the remaining 3 ML for Agar.

1 ML of MS solution per agar dish is fine right?




1 drop of MSS on a loop that is streaked across the plate is ideal.  1ml is WAY too much and will increase the likelyhood of growing contams on the plate.  Never assume anything is clean.




Okay thanks for the tip.
So as far as streaking the MSS...
Do you drop the solution on the agar, then use the loop to streak the
puddle throughout the plate?

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OfflineDERRAYLD
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Re: Is this cobweb mold? [Re: Fryingmantis]
    #27305053 - 05/12/21 12:19 AM (3 years, 8 months ago)

No, drop the solution onto the loop and streak the agar

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Invisiblepablokabute
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Re: Is this cobweb mold? [Re: Fryingmantis]
    #27305055 - 05/12/21 12:21 AM (3 years, 8 months ago)

Quote:

Fryingmantis said:
Thanks for the feedback guys.

As for my grain jars,

what would you guys do if you wanted to get things moving as quickly and sustainably as possible?

I think I may have fucked up 3 entire MS syringes.

I have 8 jars that seem to be healthy that I hope to spawn to bulk soon. Maybe next week.

And I have 1 full syringe + one with 1 ML.
I'm thinking the best move forward is to G2G the healthy jars before spawning to bulk, and in the meantime use the MS I have on agar, then transfer the agar to grain from there. One question I have.. With agar,
one petri dish can technically transfer into multiple jars right?

and the same principle with G2G? Does that mean that I could theoretically inoculate 20 more jars with one fully colonized one?

Thanks again for all the feedback. Sorry for all the mess of questions, I'm just trying to orchestrate the best way forward after finding out I wasted almost all my MS





You can extend your research by dropping a single colonized grain to agar, think of it as your insurance, if everything goes bad you can have something to work with, something to clean up while youre waiting for everything else.


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

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OfflineFryingmantis
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Re: Is this cobweb mold? [Re: pablokabute]
    #27305067 - 05/12/21 12:56 AM (3 years, 8 months ago)

Quote:

pablokabute said:
Quote:

Fryingmantis said:
Thanks for the feedback guys.

As for my grain jars,

what would you guys do if you wanted to get things moving as quickly and sustainably as possible?

I think I may have fucked up 3 entire MS syringes.

I have 8 jars that seem to be healthy that I hope to spawn to bulk soon. Maybe next week.

And I have 1 full syringe + one with 1 ML.
I'm thinking the best move forward is to G2G the healthy jars before spawning to bulk, and in the meantime use the MS I have on agar, then transfer the agar to grain from there. One question I have.. With agar,
one petri dish can technically transfer into multiple jars right?

and the same principle with G2G? Does that mean that I could theoretically inoculate 20 more jars with one fully colonized one?

Thanks again for all the feedback. Sorry for all the mess of questions, I'm just trying to orchestrate the best way forward after finding out I wasted almost all my MS





You can extend your research by dropping a single colonized grain to agar, think of it as your insurance, if everything goes bad you can have something to work with, something to clean up while youre waiting for everything else.




Oh awesome, thanks for that! I thought about that but I forgot to ask. I'll definitely do that as well :thumbup:

This made it a rough start, but my original jars seem to be doing alright, and I know with patience and perseverance there will be plenty of work to be done soon. Is it normal for MS to grain to take about a full month before full colonization? Its been around 15 days and my jars look about 1/4 to 2/4's there. From my understanding, Agar to Grain and G2G will be around 2-3 times as quick to fully colonize right?

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OfflineFryingmantis
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Re: Is this cobweb mold? [Re: DERRAYLD]
    #27305069 - 05/12/21 01:01 AM (3 years, 8 months ago)

Quote:

DERRAYLD said:
No, drop the solution onto the loop and streak the agar




Oh ok. I wasn't sure if it would, but I guess that's the point of the loop, it holds the drop in place..

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Invisiblepablokabute
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Re: Is this cobweb mold? [Re: Fryingmantis]
    #27305113 - 05/12/21 02:34 AM (3 years, 8 months ago)

You can definitely shake to speed things up. also, contaminants tend to show their true form once shaken. Like, the jar would be totally ruined, or will not recover from the shake, or grow other colors...

You can also start counting from germination day and not the inoculation day.

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OfflineDERRAYLD
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Re: Is this cobweb mold? [Re: Fryingmantis]
    #27305131 - 05/12/21 03:03 AM (3 years, 8 months ago)

Quote:

Fryingmantis said:
Quote:

DERRAYLD said:
No, drop the solution onto the loop and streak the agar




Oh ok. I wasn't sure if it would, but I guess that's the point of the loop, it holds the drop in place..




Not necessarily,  you want to minimize the amount of spores you streak to the plate, even a drop is a serious amount of spores.

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OfflineFryingmantis
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Re: Is this cobweb mold? [Re: DERRAYLD]
    #27306393 - 05/12/21 10:06 PM (3 years, 8 months ago)

so I take the needle out of the syringe before dropping it onto the loop right?

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OfflineFryingmantis
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Re: Is this cobweb mold? [Re: pablokabute]
    #27306400 - 05/12/21 10:11 PM (3 years, 8 months ago)

Quote:

pablokabute said:
You can definitely shake to speed things up. also, contaminants tend to show their true form once shaken. Like, the jar would be totally ruined, or will not recover from the shake, or grow other colors...

You can also start counting from germination day and not the inoculation day.







how long does it take for a jar to normally recover from a shake?

Edited by Fryingmantis (05/12/21 10:11 PM)

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Invisiblepablokabute
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Re: Is this cobweb mold? [Re: Fryingmantis]
    #27306530 - 05/13/21 02:41 AM (3 years, 8 months ago)

Its not uncommon to see recovery after 1 whole day, which is also a good sign of healthy, uncontaminated colony..

Shake a jar with 50-70% colonization and see it finish to 100% in 2-3 days.


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

Edited by pablokabute (05/13/21 02:41 AM)

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OfflineFryingmantis
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Re: Is this cobweb mold? [Re: pablokabute]
    #27306864 - 05/13/21 09:56 AM (3 years, 8 months ago)

Quote:

pablokabute said:
Its not uncommon to see recovery after 1 whole day, which is also a good sign of healthy, uncontaminated colony..

Shake a jar with 50-70% colonization and see it finish to 100% in 2-3 days.




Do these look okay? They had very healthy looking, connected, white growth, and now its been about 2 days after shaking. The temperature dropped from like 80 Degrees to 68 for a day though, so I think that may have slowed growth. I have the temperature back to normal now...

These are part of the group of jars that I was hoping/thinking looked okay after all.


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OfflineDERRAYLD
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Re: Is this cobweb mold? [Re: Fryingmantis]
    #27306865 - 05/13/21 09:58 AM (3 years, 8 months ago)

3rd jar on top, can you give a closer view?

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OfflineFryingmantis
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Re: Is this cobweb mold? [Re: DERRAYLD]
    #27306867 - 05/13/21 09:58 AM (3 years, 8 months ago)

Quote:

DERRAYLD said:
3rd jar on top, can you give a closer view?




Yeah hold on...

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OfflineFryingmantis
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Re: Is this cobweb mold? [Re: DERRAYLD]
    #27306879 - 05/13/21 10:10 AM (3 years, 8 months ago)

Quote:

DERRAYLD said:
3rd jar on top, can you give a closer view?



Quote:

DERRAYLD said:
3rd jar on top, can you give a closer view?




I don't know if this is the right jar/spot you were asking about,
but here's a closer look at them...

Kinda worried about them now =/


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OfflineDERRAYLD
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Re: Is this cobweb mold? [Re: Fryingmantis] * 1
    #27306887 - 05/13/21 10:15 AM (3 years, 8 months ago)

The white looking moisture on the exposed glass of the jar worried me, it had a cobweb appearance from a distance.
The jars do look wet but you did mention you had a temperature swing which can cause a lot of condensation.
They seem to be recovering from the shake but I'd watch them closely in the next few days, the shake generally exposes contamination faster in my experience.

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OfflineFryingmantis
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Re: Is this cobweb mold? [Re: DERRAYLD]
    #27306900 - 05/13/21 10:24 AM (3 years, 8 months ago)

Quote:

DERRAYLD said:
The white looking moisture on the exposed glass of the jar worried me, it had a cobweb appearance from a distance.
The jars do look wet but you did mention you had a temperature swing which can cause a lot of condensation.
They seem to be recovering from the shake but I'd watch them closely in the next few days, the shake generally exposes contamination faster in my experience.




Alright, thanks again for the feedback. I feel a bit better. I think that abrupt temperature change definitely did nothing but bad things, but now that it's dialed in I'm hoping it'll recover alright. So the condensation can't possibly introduce mold if the jar was properly PC'ed and sealed, ya?

Also, I was thinking of buying temperature-controlled heating pads to put underneath the jars during incubation... I feel like that would work better than the temperature-controlled heater I have in a cabinet... Any thoughts?

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OfflineDERRAYLD
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Re: Is this cobweb mold? [Re: Fryingmantis] * 3
    #27306913 - 05/13/21 10:31 AM (3 years, 8 months ago)

Stay away from heating pads, if af it's cold then rather make yourself a double tub with an aquarium heater in the bottom heating some water to warm the top tub.

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