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Kalypto
Psychonaut



Registered: 09/19/12
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Clarification on cultivating new strains
#27304156 - 05/11/21 10:38 AM (3 years, 8 months ago) |
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Ive been seeing a lot of unique strains going around lately and it has brought up some questions for me and something I want to clarify as well
1. When you isolate on agar then make a tub , that genetic strain will never change right ?
2. Do spores from an isolate have characteristics from the prior strain or are we back to a total crap shoot again ? I get the impression they retain some characteristics which is why people sell swabs of specific mushrooms
3. how do you cross/ combine strains? do you just put two in a petri dish and like hope they grow together in the middle ?
4. Does cloning do anything to a strain besides getting it back onto an agar dish ?
5. do you have any special tricks for finding good prolific strains ?
As always thanks for any and all help
Edited by Kalypto (05/11/21 10:38 AM)
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myc_ousin_vinny
Keeping_It_Real



Registered: 04/29/20
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Re: Clarification on cultivating new strains [Re: Kalypto]
#27304180 - 05/11/21 11:03 AM (3 years, 8 months ago) |
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Kalypto
Psychonaut



Registered: 09/19/12
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Re: Clarification on cultivating new strains [Re: myc_ousin_vinny]
#27304186 - 05/11/21 11:06 AM (3 years, 8 months ago) |
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Quote:
myc_ousin_vinny said: A strain crossing thread
Ill have to look up some monokaryons stuff
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Green_Hands
The Great Devourer



Registered: 05/21/05
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Re: Clarification on cultivating new strains [Re: Kalypto] 1
#27304190 - 05/11/21 11:10 AM (3 years, 8 months ago) |
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1. When you isolate on agar then make a tub , that genetic strain will never change right ?
Well, never is such a definitive word. Expression of genetics can change due to environment conditions but genes that you pick, that organism (lets call that strain John), will be same.
Now, everything ages and dies so your culture will also eventually do that but for the purposes of this hobby you just got John and if you get to know him you gonnna learn what he likes and what not. You got John set of genetics and it will always be John.
2. Do spores from an isolate have characteristics from the prior strain or are we back to a total crap shoot again ? I get the impression they retain some characteristics which is why people sell swabs of specific mushrooms
By printing you are limiting gene pool. So every consecutive print will narow your gene pool a bit. Children do aquire genes from parents. That process of narowing gene pool would be called domestication.
3. how do you cross/ combine strains? do you just put two in a petri dish and like hope they grow together in the middle ?
You take fresh spores from two varietes of same species and swipe them onto petri dish. You want them to germinate together so they can mate.
Than grow, grow, grow...
4. Does cloning do anything to a strain besides getting it back onto an agar dish ?
Cloning makes John.
5. do you have any special tricks for finding good prolific strains ?
Yes, grow them out and compare.
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Looping our reflections, our obsessions draw us in
Fix and fixation, no sentience beyond
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Kalypto
Psychonaut



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Re: Clarification on cultivating new strains [Re: Green_Hands]
#27304198 - 05/11/21 11:20 AM (3 years, 8 months ago) |
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Quote:
Green_Hands said:
2. Do spores from an isolate have characteristics from the prior strain or are we back to a total crap shoot again ? I get the impression they retain some characteristics which is why people sell swabs of specific mushrooms
By printing you are limiting gene pool. So every consecutive print will narow your gene pool a bit. Children do aquire genes from parents. That process of narowing gene pool would be called domestication.
cool thanks for the write up .
1. cloning doesnt domesticate correct?
2.So I got a syringe of spores if those spores had been domesticated at all is it possible that certain traits were permanently domesticated out of the strain?
3. is it possible to permanently domesticate a trait out of a strain .
4. would the domestication procedure look something like taking your best mushroom from a flush , using those spores to make agar , isolate the a new strain , then test to see if it has enhanced characteristics you were looking for ?
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Stipe-n Cap
The Pride of Cucamonga


Registered: 08/04/12
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Re: Clarification on cultivating new strains [Re: Kalypto] 1
#27304201 - 05/11/21 11:23 AM (3 years, 8 months ago) |
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Take a look at the RustyWhyte grow along thread as well. This stuff has been written down soo many times that its best to just go over the materials that have been layed out by very competent folks instead of trying to summarize everything in New threads.
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Kalypto
Psychonaut



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Re: Clarification on cultivating new strains [Re: Stipe-n Cap]
#27304207 - 05/11/21 11:26 AM (3 years, 8 months ago) |
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I totally will , I did some preliminary research on the subject but i guess i just wanst searching the right things
even having a couple people post names of authors or teks is a huge help
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Green_Hands
The Great Devourer



Registered: 05/21/05
Posts: 625
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Re: Clarification on cultivating new strains [Re: Kalypto]
#27304211 - 05/11/21 11:27 AM (3 years, 8 months ago) |
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1. cloning doesnt domesticate correct?
Correct.
2.So I got a syringe of spores if those spores had been domesticated at all is it possible that certain traits were permanently domesticated out of the strain?
If there is name on syringe than chances are that that variety has been grown for some time. Yes.
3. is it possible to permanently domesticate a trait out of a strain .
Like albinism, leucism, gnarly shape? Sure. You can select for those traits or select for lack of those traits.
4. would the domestication procedure look something like taking your best mushroom from a flush , using those spores to make agar , isolate the a new strain , then test to see if it has enhanced characteristics you were looking for ?
More or less, its a bit more complicated.
Roughly - you select traits, clone, grow, print, grow, select, clone, grow... so on and so on
It can take a lot of time.
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Looping our reflections, our obsessions draw us in
Fix and fixation, no sentience beyond
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Kalypto
Psychonaut



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Re: Clarification on cultivating new strains [Re: Green_Hands]
#27304216 - 05/11/21 11:32 AM (3 years, 8 months ago) |
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Cool cool , yeah as of right now im looking to get just a good producing strain going before i go into anything too crazy . luckily i love this hobby so time isnt a factor .
But knowing that making a custom strain can be as easy as adding two different types of spores to a plate .
I assume anything outside cubensis species wouldnt be able to integrate
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Green_Hands
The Great Devourer



Registered: 05/21/05
Posts: 625
Loc: Old World
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Re: Clarification on cultivating new strains [Re: Kalypto]
#27304225 - 05/11/21 11:42 AM (3 years, 8 months ago) |
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If you work with cubensis than only cubensis can mate with cubensis.
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Looping our reflections, our obsessions draw us in
Fix and fixation, no sentience beyond
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Kalypto
Psychonaut



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Last seen: 2 years, 11 months
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Re: Clarification on cultivating new strains [Re: Green_Hands] 1
#27304246 - 05/11/21 11:59 AM (3 years, 8 months ago) |
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Awesome thanks for the clarification im pretty sure that answers all the questions I had on the subject for at least haha
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