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OfflineLieutenant Pan
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Registered: 09/11/20
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Re: Grain jars never colonizing? [Re: horsebatterystaple]
    #27304559 - 05/11/21 04:26 PM (3 years, 8 months ago)

Until you watch the grain prep video you aren't going to understand what I tolds ya.


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InvisibleMycelium Juice
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Registered: 10/31/20
Posts: 513
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Re: Grain jars never colonizing? [Re: Lieutenant Pan]
    #27304597 - 05/11/21 04:46 PM (3 years, 8 months ago)

Quote:

horsebatterystaple said:

Grain prep:
Boil oats for 45 minutes
Dry oats on baking sheets with fan until dry




Quote:

Lieutenant Pan said:

  My guess is the oats were too dry.




Pan may be on to something...

Are a few grains burst by the time you're done boiling the oats? 

If not wait until you see a few burst grains so you know they're at max hydration.  It won't hurt anything.


Maybe you're being a little overzealous with your drying too?

I dry my oats by just throwing them in the sink for 60-90 minutes (stirring a few times to let steam dissipate).  They're always a little damp still- would prbly pass the paper towel test, but maybe not- and they always work great.  I used to pan dry like you, or use multiple strainers, but that's just a waste of time imo.

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Invisiblebakedbeings
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Re: Grain jars never colonizing? [Re: horsebatterystaple]
    #27304662 - 05/11/21 05:40 PM (3 years, 8 months ago)

Quote:

horsebatterystaple said:
It is disconcerting that a majority of jars do not have the proper conditions for what should be a successful colonization. Sure I could grain 2 grain clone in some freshly sterilized, new grain jars, but this failure rate and mode is not common, so I am hoping to uncover the root cause in my process.




true, i was leaning more toward there being a problem with your syringe, in which case g2g would bypass the problem. have you tried a g2g and been equally unsuccessful? because that would be great evidence for dry oats.

my favorite thing about oats is that you can cook the shit out of them and they still shake up easy because of their indestructable husks. when i used them i would boil them until the insides were dark and gelatinous, several separating from the husks on their own, and when i packed the jars they still looked a bit damp. it worked fine. if youre worried about too much moisture in the jar maybe put a lil verm at the bottom? but dont overdry the oats.


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Invisiblebakedbeings
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Re: Grain jars never colonizing? [Re: bakedbeings]
    #27304664 - 05/11/21 05:43 PM (3 years, 8 months ago)

you are using whole oats, right? with husks?


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Invisiblehorsebatterystaple
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Registered: 05/10/21
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Re: Grain jars never colonizing? [Re: bakedbeings]
    #27304862 - 05/11/21 08:22 PM (3 years, 8 months ago)

Yeah, whole oats. I have been killing the heat after seeing the first bursted husks on my wooden spoon, I'm worried about too many burst husks not being a good environment for the myc.

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Invisiblehorsebatterystaple
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Re: Grain jars never colonizing? [Re: Mycelium Juice]
    #27304863 - 05/11/21 08:23 PM (3 years, 8 months ago)

Quote:

Mycelium Juice said:
I dry my oats by just throwing them in the sink for 60-90 minutes (stirring a few times to let steam dissipate).  They're always a little damp still- would prbly pass the paper towel test, but maybe not- and they always work great.  I used to pan dry like you, or use multiple strainers, but that's just a waste of time imo.




This excess moisture worries me, doesn't mold and contam love the damp?

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InvisibleMycelium Juice
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Re: Grain jars never colonizing? [Re: horsebatterystaple]
    #27304946 - 05/11/21 09:36 PM (3 years, 8 months ago)

Yes, but it's not excessive by any means.  They still come out dry, and any minimal moisture left gets re-absorbed by the oats in a day or so anyway.  We're not talking straight from pot to jars here. 

If you watch a few commercial grower videos (i.e. myers mushrooms or mossy creek) those fuckers load the bags with the oats more or less still wet. I'm not going to that extreme, but they have successful businesses operating that way. 
I just don't worry too much about it anymore, and was thinking maybe it would help as you seem to be doing everything else right.


last thought/suggestion...What micron are those tyvek lids? 

You're going to want around .2 microns for gas exchange with grain spawn. If you're using .5 that can dry the grains out too quickly too.

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Invisiblehorsebatterystaple
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Registered: 05/10/21
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Re: Grain jars never colonizing? [Re: Mycelium Juice]
    #27305596 - 05/12/21 10:56 AM (3 years, 8 months ago)

Thank you for the detailed response with examples from commercial gourmet growers. I'm going to relax a bit regarding both burst husks and dry time and see how that goes for my next inoculation day.

Quote:

Mycelium Juice said:
last thought/suggestion...What micron are those tyvek lids? 





Currently running two of the 20mm adhesive dots with a .25-.3mm micron (maybe only 15mm of the filter is actually exposed to a hole in the lid, the rest is obstructed by the solid lid itself).

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